Abstract:Achieving high quality propagative material is difficult today due to the limited number of pesticides recommended for use. Simultaneously, EU regulations on Integrated Pest Management (IPM) in forest nurseries came into a force, requiring a search for alternative plant protection methods that are safe for humans, animals and the environment. In this paper, we present the possibilities of using bio-fungicides against diseases in forest nurseries, their mechanisms of action, as well as the direction of their de… Show more
“…One of such methods is the creation of optimal conditions for the development of plants and the protection of beneficial organisms that occur naturally in the ecosystem, or even by introducing natural antagonists and pathogens in the form of biopreparations [5]. Biological control is defined as the use of biopreparations, such as plant protection products containing a factor or factors of biological origin (BCA-biological control agents) to reduce the occurrence of pathogenic organisms [6]. Biotic microbial agents are living organisms that antagonize pathogens or induce plant defense mechanisms [7].…”
Fungi are important components of every ecosystem. In nature, they play a significant role as decomposers, decomposing organic matter into simple compounds available for plants. They are a curious group of organisms that attracts more and more interest not only among mycologists and phytopathologists, but also geneticists, biochemists and physiologists. Metabolic products of arboreal fungi show multidirectional action, including in the field of biological protection against harmful phytopathogens. Particularly invasive and difficult to control are soil fungi, phytobacteria and phytoviruses, which are widely spread in the rhizosphere. Moreover, the variety of pathogens is constantly increasing, and new forms are rapidly emerging, often more resistant to the biological control agents used so far.
“…One of such methods is the creation of optimal conditions for the development of plants and the protection of beneficial organisms that occur naturally in the ecosystem, or even by introducing natural antagonists and pathogens in the form of biopreparations [5]. Biological control is defined as the use of biopreparations, such as plant protection products containing a factor or factors of biological origin (BCA-biological control agents) to reduce the occurrence of pathogenic organisms [6]. Biotic microbial agents are living organisms that antagonize pathogens or induce plant defense mechanisms [7].…”
Fungi are important components of every ecosystem. In nature, they play a significant role as decomposers, decomposing organic matter into simple compounds available for plants. They are a curious group of organisms that attracts more and more interest not only among mycologists and phytopathologists, but also geneticists, biochemists and physiologists. Metabolic products of arboreal fungi show multidirectional action, including in the field of biological protection against harmful phytopathogens. Particularly invasive and difficult to control are soil fungi, phytobacteria and phytoviruses, which are widely spread in the rhizosphere. Moreover, the variety of pathogens is constantly increasing, and new forms are rapidly emerging, often more resistant to the biological control agents used so far.
“…In order to implement this method, the interaction mechanisms in the environment and also the selection of antagonistic microbial inoculants should be studied [7]. Biological control is complicated when compared to chemical methods, as it requires exact procedures for application of the Biological Control Agents (BCA's) to specific fungal pathogen and different species of plant and also understanding of both target organisms and factors involved in biological control [8]. Few studies have been dedicated to monitor the feasibility of biological control and to evaluate the activity of bacterial and fungal BCA's which could control Pythium spp.…”
Damping-off is one of the severe diseases caused by soil-borne pathogens notably Pythium sp. the causative agent of this infection in raising tree saplings in forest nurseries. Biological control is an eco-friendly approach in disease management compared to chemical fungicides which in turn affects the soil environment. Biocontrol of Pythium sp. has been emphasized in vegetable nurseries than forest nurseries. The present research work is focused on identification of effective antagonistic organism from forest nursery soils against Pythium aphanidermatum. Bacteria were isolated from various forest soils collected from Boluvampati, Sirumugai and Mettupalayam forest nurseries in Coimbatore district and soil samples were screened for antifungal activity against Pythium aphanidermatum by dual culture technique. Among 245 bacterial isolates, one isolate KUMB1.1 exhibited clear zone of inhibition of 1cm and it was identified by 16S rDNA sequencing as Streptomyces sp. Solvent extraction was performed to isolate an active compound using ethyl acetate, dichloromethane, n-butanol, hexane and chloroform in the ratio 1:1. The antifungal activity of compound was performed by well plate method against Pythium sp. and nbutanol extract exhibited zone of inhibition. The antifungal activity of Streptomyces sp. was tested in a model plant Solanum lycopersicum (Tomato) seeds raised in Pythium aphanidermatum infested soils in seed trays under in vitro conditions. Preemergence and post-emergence disease incidences were observed, and the results exhibited promising efficacy of Streptomyces sp. against the fungal pathogen Pythium aphanidermatum. Seedbed study was carried out in Gmelina arborea seeds, where the seeds are treated with Streptomyces culture broth. In which seed treatment shows 43% increase in germination compared with control.
“…A few in vivo experiments have been performed with environmental conditions simulating forest nurseries (reviewed in Okorski et al 2014;Mateos et al 2017). In addition, several ECM tested on Fusarium damping-off were poisonous to humans, for instance Paxillus involutus (Batsch ex Fr.)…”
Two isolates of the edible ectomycorrhizal fungi (ECM), Lactarius deliciosus and Rhizopogon roseolus, were tested against Fusarium oxysporum and F. verticillioides, causal agents of damping-off on seeds and seedlings of Scots pine (Pinus sylvestris) and stone pine (Pinus pinea). The effects of ECM on Fusarium spp. in the rhizosphere of Scots and stone pines were evaluated by: (1) co-inoculating ECM and Fusarium when seeding (seed test) and (2) co-inoculating eight-week-old seedlings (seedling test). The seed tests showed significant reduction of Scots pine seed germination when treated with F. verticillioides, but this effect was absent when co-inoculated with R. roseolus. Higher germination rates were observed in stone pine when F. oxysporum was co-inoculated with L. deliciosus than the pathogen inoculation alone. In the seedling test, Scots and stone pines were not apparently affected by Fusarium spp. No obvious changes in plant growth-related variables were observed in either assay. Root colonization of Scots and stone pine seedlings by R. roseolus was 15.5% and 12% for the seed assay, as well as 21.6% and 11% for the seedling assay. Lactarius deliciosus mycorrhizal roots were found only in Scots pine seedlings (10%). Rhizopogon roseolus and L. deliciosus are two promising fungi for pine seedling protection against Fusarium damping-off at nurseries.Keywords: biological control, ectomycorrhizal fungi, nursery diseases, Pinus sylvestris, Pinus pinea.
RÉSUMÉOn a testé deux isolats des champignons ectomycorhiziens comestibles (ECM) Lactarius deliciosus et Rhizopogon roseolus pour lutter contre Fusarium oxysporum et F. verticillioides, qui provoquent la fonte des graines et des semis chez le pin sylvestre (Pinus sylvestris) et le pin parasol (Pinus pinea). L' expérience consistait à évaluer l' effet de l'ECM dans la rhizosphère des pins sylvestre et parasol en (1) inoculant à la fois l'ECM et Fusarium au moment de l' ensemencement (essai sur les semences) et (2) en les inoculant aussi à des semis âgés de huit semaines (essai sur des semis). Les essais sur les semences ont montré une réduction significative de la germination chez les semences de pin sylvestre traitées avec F. verticillioides, mais cet effet disparaissait lorsqu' on inoculait aussi R. roseolus aux semences. On a aussi obtenu un taux de germination plus élevé chez le pin parasol en inoculant à la fois F. Oxysporum et L. deliciosus qu' en n'inoculant que le pathogène. Dans l' essai sur les semis, le pin sylvestre et le pin parasol n' ont pas semblé affectés par Fusarium spp. On n'a pas observé non plus d' effet sur les paramètres de croissance de la plante dans les deux essais. Dans l' essai sur les semences, le pourcentage de colonisation des racines par R. Roseolus a atteint 15,5 % chez le pin sylvestre et 12 % chez le parasol ainsi que 21,6 % et 11 % respectivement dans l' essai sur les semis. Seules les racines du pin sylvestre ont été colonisées par Lactarius deliciosus (10 %). Rhizopogon roseolus et L. deliciosus sont deux champignons pr...
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