The Possession of Three Novel Coli Surface Antigens by Enterotoxigenic Escherichia coli Strains Positive for the Putative Colonization Factor PCF8775

Abstract

A possible colonization factor, E8775, has previously been described for enterotoxigenic Escherichia coli strains of serogroups O25, O115 and O167. Re-examination of these strains by immunodiffusion has revealed that the antigenic nature of this factor, renamed putative colonization factor (PCF) 8775, is more complex than was first thought. All the strains of serogroup O25 tested possessed two antigenic components, termed CS4 and CS6, and gave mannose-resistant haemagglutination (MRHA) of human and bovine erythrocytes. Spontaneous variants possessing CS6 only did not give MRHA. Strains of serogroups O115 and O167 had the antigenic components CS5 and CS6, and gave MRHA of human, bovine and guinea-pig erythrocytes. Using immune electron microscopy, the components CS4 and CS5 were identified as fimbriae. No fimbriae were associated with CS6.

Toxins and colonization factor antigens of enterotoxigenic Escherichia coli among residents of Jakarta, Indonesia.

“…9 CFA/IV comprises antigens CS4, CS5, and CS6. 10 A number of other putative colonization factors (PCF) have been described and characterized. These include those referred to as CFA/III, CS7, CS17, PCFO159, PCFO166, PCFO148, PCFO9, and PCF 8786.…”

Prevalence of Colonization Factor Antigens (CFAs) and Adherence to HeLa Cells in Enterotoxigenic Escherichia coli Isolated from Feces of Children in São Paulo

“…Most CFAs can agglutinate different species of erythrocytes in the presence of D-mannose (19), but some, such as CFA/III, CS6, PCF020 and PCF0159, are non-hemagglutinins and can be identified only by immunologic assays (18,36,39,40).…”

“…CFA/II consists of the CS1, CS2, and CS3 subcomponents (4,32), and CFA/IV consists of the CS4, CS5, and CS6 subcomponents (39).…”

Construction of Synthetic Immunogens in View of Developing Orally-Active Anti-Enterotoxigenic E. coli Vaccines

“…These disorders, which are caused either by a thermolabile enterotoxin (LT) or by a small heat stable toxin (ST) are known to considerably impede military mobilization and deployment (2-5). The major virulence factors of ETEC have been shown to be colonization factor antigens (CFA : CFAI, CFAII and PCF 8775) (6)(7)(8)(9)(10)(11)(12). These CFA which are responsible for the attachment of bacteria to the mucosa of the small intestine and for its colonization are shared by several serogroups of E.coli.…”

“…These CFA which are responsible for the attachment of bacteria to the mucosa of the small intestine and for its colonization are shared by several serogroups of E.coli. These organisms have been shown to be good candidates for potential use in vaccines (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”