The positive impact of platelet-derived growth factor on the repair of full-thickness defects of articular cartilage

Sarban, Sezgin; Tabur, Hasan; Baba, Zeliha Füsun; Isikan, UE

doi:10.5606/ehc.2019.64018

Cited by 4 publications

(6 citation statements)

References 18 publications

(29 reference statements)

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“…The values were significantly higher than the values found after the stimulation by transforming growth factor β (TGFβ), insulin growth factor-1 (IGF-I), bFGF and HA [ 75 ]. We have already found PDGF as the most abundant growth factor in platelets and observed its positive effect on cell adhesion, migration, proliferation, and osteochondral or bone regeneration [ 97 , 98 , 99 ]. Platelets were added into the scaffold as a source of growth factors that have been found to support bone or osteochondral regeneration [ 15 , 100 , 101 , 102 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

et al. 2020

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Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol–biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.

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Section: Discussionmentioning

confidence: 99%

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

et al. 2020

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“…induce migration and proliferation of endothelial cells, as well as vascular formation [ 26 – 28 ]. Tissue restoration and reparative regeneration are promoted due to broad-spectrum action of PRP growth factors [ 5 , 13 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…As an autologous product, platelet-rich plasma is advantageous for therapeutic use as it poses no risk of side effects and no risk of transmitting infectious diseases [ 5 , 6 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Cryo-Processing on Platelet-Rich Autoplasma Preparations

et al. 2020

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Platelet-derived growth factor (PDGF) plays an important role in angiogenesis, affects activation of migration and proliferation of mesenchymal stem cells, fibroblasts, smooth muscle cells, osteoblasts; activation of migration of monocytes, macrophages, and neutrophils. The aim of the investigation was to study the effect of cryo-processing on the qualitative properties of platelet-rich autoplasma (PRP) at different time intervals. Materials and Methods. Autologous plasma preparations were obtained from the blood of 31 donors. The biological material was prepared by double centrifugation according to the protocol for obtaining P-PRP and L-PRP. Platelet count and the concentration of growth factors (PDGF-AA and PDGF-BB) were studied in fresh PRP preparations. In frozen PRP samples, the concentration of PDGF-AA and PDGF-BB was determined 2 weeks after cryo-processing and 2 months after cryo-processing at –35 °С. P-PRP and L-PRP samples activated with 10% CaCl 2 solution and those non-activated were studied. Results. L-PRP preparations are significantly superior to P-PRP preparations: the concentration of platelets is 1.7 times higher in them. The level of PDGF-AA in non-activated L-PRP is 1.8 times higher than in non-activated P-PRP (p<0.05). The level of PDGF-AA is 1.5 times higher in activated L-PRP than in activated P-PRP (p<0.05). The level of PDGF-BB is 2.9 times higher in non-activated L-PRP than in non-activated P-PRP and 1.8 times higher in activated L-PRP than in activated P-PRP (p<0.05). The concentration of PDGF-BB in non-activated P-PRP sharply increases in the 2 nd week after freezing and remains at the same level after 2 months (p<0.05). The concentration of PDGF-BB in activated plasma does not change (p>0.05). Conclusion. Cryo-processing of non-activated autologous L-PRP allows preserving and subsequently enhancing the properties of plasma concentrate, which makes it possible to apply it in clinical practice.

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“…Meanwhile, rhPDGF-BB could be used to alleviate the level of inflammation in chondrocytes and decrease the apoptosis rate of chondrocytes [ 405 ]. It has also been demonstrated that collagen sponge impregnated with PDGF was able to repair full-thickness osteochondral defect of New Zealand while rabbits [ 406 ]. Very recently, Luo et al [ 407 ] found that continuous release of PDGF from SF-HAP-based MZS could significantly increase the adhesion, proliferation, and chondrogenic differentiation of SJ-MSCs and largely improve the repair process of rabbit critical-size osteochondral defect.…”

Section: Cell Sources and Growth Factors In Mzssmentioning

confidence: 99%