The positive impact of cytological specimens for EGFR mutation testing in non‐small cell lung cancer: a single South East Asian laboratory’s analysis of 670 cases

Pang, Brendan; Matthias, D.; Ong, Chee Wee; Dhewar, A. N.; Gupta, Seema; Lim, Gune-Il; Nga, Min‐En; Seet, Ju‐Ee; Qasim, Asghar; Chin, Tan-Min; Soo, Ross A.; Soong, Richie; Salto-Tellez, Manuel

doi:10.1111/j.1365-2303.2012.01000.x

Cited by 47 publications

(76 citation statements)

References 21 publications

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“…Although, we did not compare pyrosequencing with other methods, pyrosequencing method is sensitive in detecting both EGFR and KRAS mutations. Similar to previous reports, EGFR mutations were prevalent in nonsmokers (65.43%, P < 0.001), whereas KRAS mutations were common in smokers (67.85%, P < 0.001), and the two mutations are mutually exclusive in our study (6)(7)(8)25,29).…”

Section: Discussionsupporting

confidence: 91%

“…The utility of cytology samples in detecting mutations in lung cancer have recently been increased and relatively widely investigated (8,(30)(31)(32). Cytology samples are especially useful in The present study showed that cytology specimens are comparable, or even better than non-cytology specimens, in detecting both EGFR and KRAS mutations.…”

Section: Discussionsupporting

confidence: 50%

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446 Detection of EGFR and KRAS Mutation by Pyrosequencing Analysis in Cytologic Samples of Non-Small Cell Lung Cancer

Kim¹,

Lee²,

Oh³

et al. 2012

European Journal of Cancer

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 50%

446 Detection of EGFR and KRAS Mutation by Pyrosequencing Analysis in Cytologic Samples of Non-Small Cell Lung Cancer

Kim¹,

Lee²,

Oh³

et al. 2012

European Journal of Cancer

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“…The sensitivity of molecular application on cytological material may vary depending on the methodology used, which raises the need to properly validate the molecular procedure in every laboratory [33,34]. Fine-needle aspiration biopsy sampling represents an effective alternative with high DNA and RNA quality even in the close future with the perspective of obtaining neoplastic cells for genome sequencing using next-generation sequencing and outstanding results in the lung cancer field, too [35].…”

Section: Discussionmentioning

confidence: 99%

Detection of Common and Less Frequent <b><i>EGFR</i></b> Mutations in Cytological Samples of Lung Cancer

Rossi

Gerhard²,

Cirnes³

et al. 2014

Acta Cytologica

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Objective: Lung cancer represents the leading cause of cancer death. EGFR mutations, detectedin 10-40% of lung adenocarcinomas, are an essential key to therapeutic management. EGFR-activated mutations comprise mainly deletions in exon 19 and point mutations in exon 21. Although histology is the traditional method of detection,we investigated the role of cytology in EGFR mutations. Study Design: A total of 774 lung cancers were studied for EGFR mutations (676 histological and 98 cytological samples), including 424 adenocarcinomas, 326 non-small cell lung carcinomas not otherwise specified, and 24 squamous cell carcinomas. Results: We had a total of 164 (21.2%) cases of mutations. Common mutations were short in-frame deletions in exon 19 (53.7%) and single-nucleotide substitutions in exon 21 (34.1%); less frequent mutations included single-nucleotide substitutions in exon 18 (3.7%) and in-frame insertions/deletions in exon 20 (8.5%). Histologically, EGFR mutations in exons 19 and 21 occurred in 19.4% and in exons 18 and 20 in 2.2%, while the rates cytologically were 13.3% for exons 19 and 21 and 5.1% for exons 18 and 20. Conclusions: The sensitivity for the detection of EGFR mutations in cytological samples overlaps histology, so the use of cytological material constitutes an adequate approach for treatment selection in patients with locally advanced or metastatic lung cancer.

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“…First, the nature of retrospective study will induce the collection bias. Second, the cytological samples varies, thus the tumor cell numbers, tumor percentages or DNA concentrations differs, which might affect the sensitivity of EGFR mutation detection (Pang et al, 2012;Khode et al, 2013;Jing et al, 2013). Third, not all of paired samples were collected simultaneously at a same location, the phenomenon of heterogeneity could has an impact on the detection result of EGFR mutation.…”

Section: Concordance Rate Of Egfr Mutations Between 101 Lbc Samples Amentioning

confidence: 99%

High Feasibility of Liquid-Based Cytological Samples for Detection of EGFR Mutations in Chinese Patients with NSCLC

Wu¹,

Hou²,

Ren³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Activating mutations of epidermal growth factor receptor (EGFR) could predict response to tyrosine kinase inhibitor (TKI) treatment in patients with non-small cell lung cancer (NSCLC). However, the detection of EGFR mutation is frequently challenging in clinical practice for the lack of tumor tissue. The aim of this study was to investigate the feasibility of performing EGFR mutation testing on various types of liquid-based cytology (LBC) samples. Materials and Methods: A total of 434 liquid-based cytology samples were collected from March 2010 and November 2013. Among them, 101 with diagnosis of lung adenocarcinoma had paired surgically resected specimens. The ADx Amplification Refractory Mutation System (ADx-ARMS) was used to determine EGFR mutation status both in LBC and resected samples. Results: All liquid-based cytology samples were adequate for EGFR mutation analysis. The mutation rate was 50.5% in the 434 NSCLC patients with LBC samples and the incidence rates of EGFR mutation were consistent among different specimens. We also detected EGFR positives in 52.5% (53/101) patients with paired histologic specimens. The concordance rate of EGFR mutation between LBC samples and paired histologic specimens was 92.1%. Conclusions: Our results suggest that liquid-based cytology samples are highly reliable for EGFR mutation testing in patients with NSCLC.

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The positive impact of cytological specimens for EGFR mutation testing in non‐small cell lung cancer: a single South East Asian laboratory’s analysis of 670 cases

Cited by 47 publications

References 21 publications

446 Detection of EGFR and KRAS Mutation by Pyrosequencing Analysis in Cytologic Samples of Non-Small Cell Lung Cancer

446 Detection of EGFR and KRAS Mutation by Pyrosequencing Analysis in Cytologic Samples of Non-Small Cell Lung Cancer

Detection of Common and Less Frequent <b><i>EGFR</i></b> Mutations in Cytological Samples of Lung Cancer

High Feasibility of Liquid-Based Cytological Samples for Detection of EGFR Mutations in Chinese Patients with NSCLC

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