The positional distributions of fatty acids in the triacylglycerols and phosphatidylcholines of the intestinal and popliteal lymph and plasma of sheep

Christie, William W.; Clegg, Roger A.; Calvert, D. T.; Noble, R. C.

doi:10.1007/bf02534739

Cited by 11 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, methods based on reversed‐phase high‐performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry (RP‐HPLC/APCI‐MS),22–29 and on RP‐HPLC coupled with electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS),30–33 have been developed for positional analysis of TAGs. These methods are less laborious than traditional methods34–46 that require fractionation of TAGs, fraction collection, enzymatic digestion of each fraction, and the separation and detection of the diglycerides (DAGs) and free fatty acid hydrolysis products in each of the digested fractions. RP‐HPLC provides superb fractionation of complex TAG mixtures, and on‐line mass spectrometric analysis yields information about fatty acid composition and position.…”

mentioning

confidence: 99%

Examining the collision‐induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. I. The OXO/YOY series

Evans

2005

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

A series of positionally pure triglycerides (TAGs) of the form OXO and YOY, where O is the oleate moiety and X and Y are large arrays of different fatty acid moieties, was synthesized and analyzed by reversed-phase high-performance liquid chromatography/tandem mass spectrometry. The intensities of the collision-induced decomposition (CID) products of ammoniated TAGs (ammonium ion adducts) were examined as a function of chain length, degree of unsaturation, double-bond position, and cis/trans configuration of X and Y. The major CID products, the diglyceride fragment ions and the MH+ ion, were plotted as functions of chain length for the saturated and mono-unsaturated series of X and Y. Different trends for each of these series were observed. Trends in the abundances of these fragment ions were also characterized as a function of degree of unsaturation in the TAGs. In general, the fractional abundances of the MH+ ions vary linearly with degree of unsaturation. However, the presence of double bonds positioned close to the carbonyl carbon of the fatty acid chain promotes the formation of the diglyceride fragment ion corresponding to loss of that fatty acid. Mechanisms of the formation and decomposition of ammoniated TAGs are proposed that fit the trends observed in the data. Extensions of this work are described, and a vision of a derived library of CID spectra is discussed as a platform for comprehensive analysis of complex TAG mixtures.

show abstract

mentioning

confidence: 99%

Examining the collision‐induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. I. The OXO/YOY series

Evans

2005

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…Recently, reversed‐phase high‐performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (RP‐HPLC/APCI‐MS)1–8 and reversed‐phase high performance liquid chromatography/electrospray tandem mass spectrometry (RP‐HPLC/ESI‐MS/MS)9–12 methods have been developed for positional analysis of triglycerides (TAGs). These methods are less laborious than traditional methods,13–25 which require fractionation of TAGs, fraction collection, enzymatic digestion of each fraction, and the separation and analysis of the diglycerides (DAGs) and free fatty acid hydrolysis products in each of the digested fractions. RP‐HPLC provides superb fractionation of complex TAG mixtures, and on‐line MS analysis yields information about fatty acid composition and position.…”

mentioning

confidence: 99%

Examining the collision‐induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. II. The PXP/YPY series

Collins

Evans

2005

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

A series of positionally pure triglycerides (TAGs) of the form PXP and YPY, where P is the palmitate moiety and X and Y are large arrays of different fatty acid moieties, is synthesized and analyzed by reversed-phase high-performance liquid chromatography/tandem mass spectrometry. The intensities of the collision-induced decomposition (CID) products of ammoniated TAGs were examined as a function of chain length, degree of unsaturation, double-bond position, and cis/trans configuration of X and Y. The major CID products, the diglyceride (DAG) fragment ions and the MH(+) ions, are plotted as functions of chain length for the saturated and monounsaturated series of X and Y. Different trends for each of these series are observed. Trends in the intensities of these fragment ions are also characterized as a function of degree of unsaturation in the TAGs. In general, the fractional intensities of MH(+) increase with increasing degree of unsaturation in the TAGs. MH(+) is absent in the CID spectra of triglycerides containing three saturated fatty acid moieties, suggesting that the presence of double bonds fosters the formation of MH(+). Double bonds positioned close to the carbonyl carbon along the fatty acid chain promote the formation of the DAG fragment ion corresponding to the loss of the fatty acid. The collection of PXP/YPY data produced in this work is used to test the mechanisms of the formation and decomposition of ammoniated TAGs that were previously presented. The YPY data are used to predict the intensities of the fragment ions in the CID spectra of YPX-type TAGs. The limitations of the mathematical approach used in these predictions are discussed in context of a broader plan to develop a software platform for comprehensive analysis of complex TAG mixtures.

show abstract

“…Fatty acid methyl esters (FAME) analysis was carried out using the samples prepared from the TLC purified sn-2 MAG from TAG, and the sn-1 FFA and sn-2 lyso-MGDG/DGDG from galactolipids by GC analysis. It should be noted that the fatty acid composition of the sn-1/3 FFA released from TAG was calculated as previously described in Christie et al (1984). The digestion of galactolipids using the same methods as the TAG was previously reported by Yongmanitchai and Ward (1993).…”

Section: Lipid Classes Characterizationmentioning

confidence: 99%

A Synergistic Genetic Engineering Strategy Induced Triacylglycerol Accumulation in Potato (Solanum tuberosum) Leaf

Akbar

Shrestha

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Potato is the 4th largest staple food in the world currently. As a high biomass crop, potato harbors excellent potential to produce energy-rich compounds such as triacylglycerol as a valuable co-product. We have previously reported that transgenic potato tubers overexpressing WRINKLED1, DIACYLGLYCEROL ACYLTRANSFERASE 1, and OLEOSIN genes produced considerable levels of triacylglycerol. In this study, the same genetic engineering strategy was employed on potato leaves. The overexpression of Arabidopsis thaliana WRINKED1 under the transcriptional control of a senescence-inducible promoter together with Arabidopsis thaliana DIACYLGLYCEROL ACYLTRANSFERASE 1 and Sesamum indicum OLEOSIN driven by the Cauliflower Mosaic Virus 35S promoter and small subunit of Rubisco promoter respectively, resulted in an approximately 30-fold enhancement of triacylglycerols in the senescent transgenic potato leaves compared to the wild type. The increase of triacylglycerol in the transgenic potato leaves was accompanied by perturbations of carbohydrate accumulation, apparent in a reduction in starch content and increased total soluble sugars, as well as changes of polar membrane lipids at different developmental stages. Microscopic and biochemical analysis further indicated that triacylglycerols and lipid droplets could not be produced in chloroplasts, despite the increase and enlargement of plastoglobuli at the senescent stage. Possibly enhanced accumulation of fatty acid phytyl esters in the plastoglobuli were reflected in transgenic potato leaves relative to wild type. It is likely that the plastoglobuli may have hijacked some of the carbon as the result of WRINKED1 expression, which could be a potential factor restricting the effective accumulation of triacylglycerols in potato leaves. Increased lipid production was also observed in potato tubers, which may have affected the tuberization to a certain extent. The expression of transgenes in potato leaf not only altered the carbon partitioning in the photosynthetic source tissue, but also the underground sink organs which highly relies on the leaves in development and energy deposition.

show abstract

The positional distributions of fatty acids in the triacylglycerols and phosphatidylcholines of the intestinal and popliteal lymph and plasma of sheep

Cited by 11 publications

References 15 publications

Examining the collision‐induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. I. The OXO/YOY series

Examining the collision‐induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. I. The OXO/YOY series

Examining the collision‐induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. II. The PXP/YPY series

A Synergistic Genetic Engineering Strategy Induced Triacylglycerol Accumulation in Potato (Solanum tuberosum) Leaf

Contact Info

Product

Resources

About