The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria

Pagès, Jean‐Marie; James, Chloë E.; Winterhalter, Mathias

doi:10.1038/nrmicro1994

Cited by 753 publications

(783 citation statements)

References 94 publications

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“…Both efflux pumps and porins contribute to microbial resistance to toxic compounds, such as antibiotics (Davin-Regli et al 2008;Delcour 2009;Pages et al 2008). In the study by Khudyakov et al, some of the efflux pumps and porins were up-regulated and down-regulated, respectively, in E. lignolyticus cells exposed to…”

Section: Elucidating Mechanisms Of Il Tolerancementioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Elucidating Mechanisms Of Il Tolerancementioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…The lack of new drugs combined with the rapid spread of resistant organisms poses a tremendous challenge for medical research and practice in the 21 st century. Antibiotic resistance develops in a number of ways: the lipid membrane can act as a barrier preventing the accumulation of the drug in the cell, the drugs might be enzymatically decomposed, the drug targets can be modified; in many cases multiple mechanisms are active simultaneously 3,4 . A better understanding of drug transport across lipid membranes is highly desirable and could facilitate the development of the next generation of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

et al. 2014

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With the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes is gaining widespread importance. We present a novel, label-free microfluidic assay that quantifies the permeability coefficient of a broad spectrum fluoroquinolone antibiotic, norfloxacin, across lipid membranes using the UV autofluorescence of the drug. We use giant lipid vesicles as highly controlled model systems to study the diffusion through lipid membranes. Our technique directly determines the permeability coefficient without requiring the measurement of the partition coefficient of the antibiotic.

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“…First steps in this direction are already under active development [113]. It is also conceivable that these experiments would help investigations about antibiotics transport into bacteria [114,115].…”

Section: Future Perspectivesmentioning

confidence: 99%

Controlling molecular transport through nanopores

Keyser

2011

J. R. Soc. Interface.

172

191

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Nanopores are emerging as powerful tools for the detection and identification of macromolecules in aqueous solution. In this review, we discuss the recent development of active and passive controls over molecular transport through nanopores with emphasis on biosensing applications. We give an overview of the solutions developed to enhance the sensitivity and specificity of the resistive-pulse technique based on biological and solid-state nanopores.

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The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria

Cited by 753 publications

References 94 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

Controlling molecular transport through nanopores

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