The pore size of mesoporous silica nanoparticles regulates their antigen delivery efficiency

Abstract

Subunit vaccines generally proceed through a 4-step in vivo cascade—the DUMP cascade—to generate potent cell-mediated immune responses: (1) drainage to lymph nodes; (2) uptake by dendritic cells (DCs); (3) maturation of DCs; and (4) Presentation of peptide-MHC I complexes to CD8+ T cells. How the physical properties of vaccine carriers such as mesoporous silica nanoparticles (MSNs) influence this cascade is unclear. We fabricated 80-nm MSNs with different pore sizes (7.8 nm, 10.3 nm, and 12.9 nm) and loaded them with ovalbumin antigen. Results demonstrated these MSNs with different pore sizes were equally effective in the first three steps of the DUMP cascade, but those with larger pores showed higher cross-presentation efficiency (step 4). Consistently, large-pore MSNs loaded with B16F10 tumor antigens yielded the strongest antitumor effects. These results demonstrate the promise of our lymph node-targeting large-pore MSNs as vaccine-delivery vehicles for immune activation and cancer vaccination.

Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering

“…MSNs have good biocompatibility, high drug-loading capacity and good drug release performance. Their mesoporous structure has a good protective effect against peptide drugs [ 16 ].…”

Short-Term Oral Administration of Mesoporous Silica Nanoparticles Potentially Induced Colon Inflammation in Rats Through Alteration of Gut Microbiota

“…The parameters like surface charge, 23 pore size, 23 , 24 pore channel structure, 15 particle shape, 10 , 25 particle size, 26 and even exposure routes 9 all matters when investigating the toxicity of MSNs. Herein, we prepared three MSNs with same surface charge (negatively charged) and similar pore size (~3 nm), with further administrated with the same exposure route (i.g.).…”

Progress in Mesoporous Silica Nanoparticles as Drug Delivery Agents for Cancer Treatment

“…Therefore, researchers have dedicated their work to expand the pore size. One study reported that the pore size could be adjusted by changing the concentration of TEOS in cyclohexane, and they managed to obtain MSNs with pore sizes ranging from 7.8 nm to 12.9 nm [ 49 ]. Liu and coworkers synthesized dendrimers like MSNs, where the MSNs exhibited center-radial pores with the gradual increase of pore size from the interior to the surface of MSNs.…”