The population of Sclerotinia sclerotiorum affecting common bean in Brazil is structured by mycelial compatibility groups

White mold is a highly destructive disease caused by Sclerotinia sclerotiorum, a polyphagous fungus that infects several hosts including common bean grown that is widely cultivated in Brazil. The pathogenic populations are known to exhibit genetic variability but studies rarely used single ascospore-derived strains. In this study, 12 monoascospore strains originated from a same isolate (UFLA 54), but from different apothecia, were compared among them and with the mother strain with regards: days to first sclerotium formation, mycelial growth rate mycelial compatibility groups, microsatellite markers and mating type locus (MAT1-1 and MAT1-2). Their pathogenicity was assessed on three common bean lines. Cluster analysis of growth rates data separated UFLA 54 from all monoascospore strains, which shared same alleles in the microsatellite locus. Inversion of MAT1-1 region was found for both UFLA 54 and half of monoascospore strains. Aggressiveness of the monoascospore strains was not consistent across common bean lines. Our study reveals significant variability of S. sclerotiorum at the single isolate level. Keywords Phaseolus vulgaris. White mold. Aggressiveness. Morphological traits White mold is a fungal disease caused by Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic and homothallic fungus capable of infecting several hosts and causing severe epidemics in important field crops including soybean, common bean and sunflower. White mold epidemics are reported worldwide, including Brazil, with yield losses estimated at 19% to 70% depending on the geographic region and year (

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The Population of Sclerotinia sclerotiorum in Brazil Is Structured by Mycelial Compatibility Groups

Silva

Ferro

Lehner

et al. 2021

Plant Disease

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The genetic structure of the population of Sclerotinia sclerotiorum was analyzed using 238 individuals collected from different hosts. Individuals were characterized for microsatellite genotypes and mycelial compatibility groups (MCGs). A total of 22 MCGs and 64 multilocus lineages (MLLs) were identified. There was a close relationship between the MCGs and MLLs, but there was no association between MLLs and hosts or regions. At least 39 MCGs are present in Brazil and 68.5% of the isolates were assigned to either MCG 1 or 2. Eight new MCGs were found. Seven genetic groups were identified and associated with MCGs. Most genetic variation (70.0%) was due to differences among MCGs. High values of estimates of linkage disequilibrium among loci were more frequent in the total population (all MCGs). In contrast, there was evidence of random mating in subpopulations defined by MCGs 1 and 2. Additionally, there was evidence of outcrossing in the population of S. sclerotiorum in Brazil. The population was structured by MCGs, lineages originated from asexual reproduction or selfing prevail and are widely distributed in space, are persistent in time and affect many hosts, but there is evidence of some degree of outcrossing which may lead to a more genetically variable population in the future.

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The population of Sclerotinia sclerotiorum affecting common bean in Brazil is structured by mycelial compatibility groups

Cited by 4 publications

References 45 publications

Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina

Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina

Phenotypic and genotypic characterization of single isolate-derived monoascospore strains of Sclerotinia sclerotiorum from common bean

The Population of Sclerotinia sclerotiorum in Brazil Is Structured by Mycelial Compatibility Groups

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