The population growth, development and metabolic enzymes of the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae) under the sublethal dose of triflumezopyrim

Chen, Lin; Wang, Xuegui; Zhang, Yunzheng; Yang, Rui; Zhang, Shuirong; Xu, Xin; Zhu, Mengjin; Gong, Changwei; Hasnain, Ali; Shen, Litao; Jiang, Chengyao

doi:10.1016/j.chemosphere.2020.125865

Cited by 27 publications

(20 citation statements)

References 37 publications

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“…Prior studies have also demonstrated that Vg upregulation promotes Vg protein synthesis in the fat bodies of N. lugens adult females, and stimulates fecundity of N. lugens adult females 16,72 . However, recent studies also indicated TFM treatment increased Vg , VgR , and EcR expression level and contents in F 4 female adults in Sogatella furcifera , compared with the F 0 generation, promoting growth and reproduction in S. furcifera 73 . When seeds were pretreated with TFM, N. lugens females had lower JH III titers (Figure 6C), which resulted in reduced Vg levels (Figure 7E), body weight (Figure 6E), and lower fecundity (Figure 9A) compared with females feeding on untreated controls.…”

Section: Discussionmentioning

confidence: 97%

Seed dressing with triflumezopyrim controls brown planthopper populations by inhibiting feeding behavior, fecundity and enhancing rice plant resistance

Zhang²,

Chen

et al. 2021

Pest Management Science

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BACKGROUND Triflumezopyrim (TFM), a novel mesoionic insecticide, has high efficiency at a low dosage, and is mainly used to control hopper species. A previous study demonstrated that seed dressing with TFM effectively controlled rice planthopper populations in mechanically transplanted rice fields; however, mode of action for control was unclear. RESULTS The study shows that seed dressing with TFM resulted in elevated levels of oxalic acid, flavonoids, phenolic substances, callose and other compounds associated with Nilaparvata lugens resistance in rice plants, and low TFM residue content in rice plant stem and grain. Host choice behavioral experiments showed that N. lugens females prefer feeding on untreated rice plants. Electrical penetration graph (EPG) data showed that seed dressing with TFM at medium and high doses significantly prolonged the non‐probing period and inhibited phloem ingestion in N. lugens females. These changes led to a significant decrease in female secretion of honeydew, expression of genes encoding vitellogenin and juvenile hormone acid methyltransferase, body weight and longevity, and significantly influenced several physiological parameters resulting in impaired oocyte growth, fecundity and population. Field survey data showed that seed dressing with TFM was efficacious and relatively durable in protecting rice plants from infestation by planthoppers. CONCLUSION This study revealed that seed dressing with TFM enhances rice plant resistance to N. lugens by limiting phloem ingestion and increasing the N. lugens non‐probing period; this leads to reduced fecundity of females and lowers N. lugens numbers in the field. © 2021 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 97%

Seed dressing with triflumezopyrim controls brown planthopper populations by inhibiting feeding behavior, fecundity and enhancing rice plant resistance

Zhang²,

Chen

et al. 2021

Pest Management Science

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“…The indoor resistant population was screened by the rice seedling impregnation method, with little modification [ 16 , 31 ]. Third instar nymphs were used in the study.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA for RT-qPCR of the Lab-HN strain and XY17 fifth- (G5) and sixth (G6)-generation populations were extracted by the traditional method by Chen et al [ 31 ]. The CDS sequences of ten insecticide resistant-related P450 genes ( CYP6CS3 , CYP6AX3, CYP408A3 , CYP4CE3 , CYP417A4 , CYP4DD1 , CYP18A1 , CYP408A3 , CYP6ER4, and CYP418A2 ) were obtained from the research of Yang et al [ 27 ], which referred to the transcriptome of S. furcifera by Wang et al [ 32 ]; one reference gene ( RPL9 ) [ 33 ] from the Lab-HN strain and the XY17 population each were amplified through RT-qPCR, with eleven pairs of corresponding primers ( Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

Cloning and Functional Verification of CYP408A3 and CYP6CS3 Related to Chlorpyrifos Resistance in the Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

Ruan

Liu

Gong

et al. 2021

Biology

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The white-back planthopper (WBPH), Sogatella furcifera, mainly harms rice and occurs in most rice regions in China and Asia. With the use of chemical pesticides, S. furcifera has developed varying degrees of resistance to a variety of pesticides. In our study, a chlorpyrifos-resistant population (44.25-fold) was built through six generations of screening with a sublethal dose of chlorpyrifos (LD50) from a field population. The expression levels of ten selected resistance-related P450 genes were analyzed by RT-qPCR and found that CYP408A3 and CYP6CS3 were significantly more expressed in the third instar nymphs of the XY17-G5 and XY17-G6 populations, about 25-fold more than the Sus-Lab strain, respectively (p < 0.01). To elucidate their molecular function in the development of resistance towards chlorpyrifos, we cloned two P450 full lengths and predicted their tertiary protein structures. CYP408A3 and CYP6CS3 were also downregulated after injecting dsCYP408A3, dsCYP6CS3, or their mixture compared to the control group. Moreover, the mortality rates of the dsCYP6CS3 (91.7%) and the mixture injection treatment (93.3%) treated by the LC50 concentration of chlorpyrifos were significantly higher than the blank control group (51.7%) and dsCYP408A3 injection treatment (69.3%) at 72 h (p < 0.01). Meanwhile, the P450 enzyme activities in the dsRNA treatments were lower than that in the control (XY17-G6) (p < 0.01). Therefore, the P450 gene CYP6CS3 may be one of the main genes in the development of chlorpyrifos resistance in S. furcifera.

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“…The ovaries of the Bis-UNSEL and Bis-SEL strains that had pupated on days 5, 6, 7 or 8 or emerged at 0, 12, 24, 36, 48, 60 or 72 h were collected. Extractions of Vg and VgR were improved by the method of Zhen et al 37 According to the instructions of the ELISA kit (Product Code mlbio104703 for insect vitellogenin, mlbio104704 for insect vitellogenin receptor; Shanghai Enzyme Biotechnology Co. Ltd, Shanghai, China), the protein contents of Vg and VgR were determined by the method described by Chen et al 24 Absorbance was measured at 450 nm with a microplate reader (Model 680 Microplate Reader, Bio-Rad), and the concentrations of Vg (or VgR) were determined by comparing the OD of the samples to the standard curve.…”

Section: Determination Of Contents Of Vg Vgr and Chitinmentioning

confidence: 99%

“…21,22 High-concentration imidacloprid treatment significantly reduced the parasitic rate of Anagrus nilaparvatae, 23 and a sublethal dose of triflumezopyrim stimulated the reproduction of Sogatella furcifera. 24 After triazophos treatment, the oviposition and egg hatching rate of Nilaparvata lugens were significantly increased. 25,26 Validamycin treatment also stimulated the reproduction of N. lugens.…”

Section: Introductionmentioning

confidence: 96%

The fitness advantages of bistrifluron resistance related to chitin synthase A in Spodoptera litura (Fab.) (Noctuidae: Lepidoptera)

Gong

Wang

Huang

et al. 2021

Pest Management Science

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BACKGROUND: Spodoptera litura is one of the major agricultural pests in China, and it has developed serious resistance to many traditional chemical insecticides. In the present study, the bistrifluron-resistant (Bis-SEL) strain accompanied by a higher oviposition, 113.8-fold RR compared to the bistrifluron-susceptible (Bis-UNSEL) strain, was obtained by bidirectional screening. A comparison of their gonad coefficiency and genes related to oviposition or resistance was used to elucidate the resurgence mechanism.RESULTS: The ovarian index, oviposition, and potential egg production in the Bis-SEL strain of female adults were significantly higher than those in the Bis-UNSEL strain, and the length of ovariole in the Bis-SEL strain was also significantly elongated. The protein contents of vitellogenin (Vg) and vitellogenin receptor (VgR) in the Bis-UNSEL strain were lower than those in the Bis-SEL strain, consistent with their gene expressions levels, and there was a significantly positive linear correlation between Vg and VgR protein contents, further confirming that resistant strains have high reproductive fitness. Moreover, the chitin synthase A in the Bis-SEL strain was clearly up-regulated, and a mutation (H866Y) near the QRRRW in the catalytic domain caused a rise in the hydrogen bond between UDP-GlcNAc and chitin synthase, and its chitin content was higher than that in the Bis-UNSEL strain. Nevertheless, the sensitivity of the Bis-SEL strain to bistrifluron was significantly recovered when it was knocked down though RNA interference. CONCLUSION:The fitness advantages of bistrifluron resistance may be related to the up-regulation and mution of chitin synthase A.

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The population growth, development and metabolic enzymes of the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae) under the sublethal dose of triflumezopyrim

Cited by 27 publications

References 37 publications

Seed dressing with triflumezopyrim controls brown planthopper populations by inhibiting feeding behavior, fecundity and enhancing rice plant resistance

Seed dressing with triflumezopyrim controls brown planthopper populations by inhibiting feeding behavior, fecundity and enhancing rice plant resistance

Cloning and Functional Verification of CYP408A3 and CYP6CS3 Related to Chlorpyrifos Resistance in the Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

The fitness advantages of bistrifluron resistance related to chitin synthase A in Spodoptera litura (Fab.) (Noctuidae: Lepidoptera)

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