The Population Genetic Structure of Tetranychus kanzawai Kishida (Acari: Tetranychidae) in Northern Hokkaido, Japan, as Estimated Using Microsatellite Polymorphism

慎哉, 西村; 典秀, 日本; 晃雄, 高藤

doi:10.2300/acari.16.11

Cited by 5 publications

(6 citation statements)

References 34 publications

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“…These results suggest that gene flow among populations is limited. An analysis by Nishimura et al (2007) using microsatellite markers also found that the genetic diversity among populations was relatively high in this area. The restricted gene flow will accelerate genetic diversification among the populations, as was shown in the present study.…”

Section: Discussionmentioning

confidence: 68%

DNA Sequence Variation in the Tetranychus kanzawai Complex in Northern Hokkaido, Japan

Hinomoto¹,

Nishimura²,

Takafuji³

2007

J. Acarol. Soc. Jpn.

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DNA sequences of the internal transcribed spacer 1 (ITS1) of the nuclear ribosomal RNA gene and of mitochondrial cytochrome oxidase subunit I (COI) were analyzed for 13 Tetranychus kanzawai populations collected in northern Hokkaido, Japan. Five and 13 DNA haplotypes were found for ITS1 and COI, respectively, and the COI haplotypes were classified into 5 amino acid haplotypes. Monophyly of all haplotypes found in this study was strongly supported by bootstrap analysis, confirming that all analyzed individuals belonged to the T. kanzawai complex. The geographic distributions of these haplotypes overlapped, showing that populations had differentiated genetically from each other. However, some hybrid individuals were detected, indicating that genetic differentiation was not complete. Large genetic variation in the DNA sequences in the study area and the discovery of ancestral haplotypes strongly suggest that the complex originated in Northeast Asia.

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Section: Discussionmentioning

confidence: 68%

DNA Sequence Variation in the Tetranychus kanzawai Complex in Northern Hokkaido, Japan

Hinomoto¹,

Nishimura²,

Takafuji³

2007

J. Acarol. Soc. Jpn.

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“…We examined the diversity of markers isolated in this study (TtMS) and of the four markers previously isolated from T. kanzawai (TkMS; Nishimura et al, 2003Nishimura et al, , 2007 in both the Kyoto and the Bac Ninh populations. We used 24 females of the Kyoto population and 27 of the Bac Ninh population for the genotyping PCR.…”

Section: Characteristics Of the Microsatellite Markersmentioning

confidence: 99%

Development and Evaluation of Microsatellite Markers in Tetranychus truncatus Ehara (Acari: Tetranychidae)

Hinomoto¹,

Higaki²,

Osakabe³

et al. 2009

J. Acarol. Soc. Jpn.

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“…One primer of each microsatellite locus was modified with one of three fluorescent dyes and used in multiplex PCR amplification as described by Nishimura et al (2007). Sequence data analysis DNA sequences were aligned by using Clustal W software (Thompson et al, 1994), and phylogenetic analysis was performed by the neighbor-joining method with PAUP* software (version 4.0b10; Swofford, 2000).…”

Section: Study Sites and Mitesmentioning

confidence: 99%

“…In contrast, Nishimura et al (2007) analyzed T. kanzawai populations collected from the same host plant species in northern Hokkaido, in northern Japan, at the regional scale and revealed that geographic barriers were not responsible for the high levels of genetic diversification in the mite populations. To confirm the effect of host plant on the genetic divergence of T. kanzawai populations, additional genetic investigations should be done in other study areas and on different scales.…”

Section: Introductionmentioning

confidence: 97%

“…Microsatellite markers also have been isolated from, and developed for, several spider mite species (Bailly et al, 2004;Navajas et al, 1998b;Nishimura et al, 2003;Osakabe et al, 2000;Uesugi and Osakabe, 2007;Weeks et al, 2001). Their high levels of polymorphism have revealed marked intraspecific variations within and among populations, and currently the microsatellite markers have facilitated several studies of population genetics of spider mites (Bailly et al, 2004;Carbonnelle et al, 2007;Groot et al, 2005;Navajas et al, 2002;Nishimura et al, 2005Nishimura et al, , 2007Osakabe et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The Genetic Variations among Populations of Tetranychus kanzawai Complex (Acari: Tetranychidae) Indicated by Using Mitochondrial, Ribosomal, and Microsatellite DNA Markers

慎哉¹,

典秀²,

晃雄³

2007

J. Acarol. Soc. Jpn.

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We used two types of molecular markers to analyze the genetic structure of seven populations of Tetranychus kanzawai complex collected from the Tohoku area of northern Honshu, the main island of Japan. For phylogenetic analysis, we used DNA sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene and the internal transcribed spacer 1 (ITS1) region of the ribosomal RNA gene; for detailed analysis of population structure, we used microsatellite markers. We found that all population pairs were differentiated significantly; this differentiation was enhanced significantly by host plant effect but not by geographic distance among the populations. DNA sequencing of ITS1 and COI regions and microsatellite analysis both showed that the population occurring on Pueraria lobata was deviated genetically from the other populations. Therefore, our analysis suggested that population subdivision in T. kanzawai complex developed because of host plant effects rather than geographic barriers.

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The Population Genetic Structure of Tetranychus kanzawai Kishida (Acari: Tetranychidae) in Northern Hokkaido, Japan, as Estimated Using Microsatellite Polymorphism

Cited by 5 publications

References 34 publications

DNA Sequence Variation in the Tetranychus kanzawai Complex in Northern Hokkaido, Japan

DNA Sequence Variation in the Tetranychus kanzawai Complex in Northern Hokkaido, Japan

Development and Evaluation of Microsatellite Markers in Tetranychus truncatus Ehara (Acari: Tetranychidae)

The Genetic Variations among Populations of Tetranychus kanzawai Complex (Acari: Tetranychidae) Indicated by Using Mitochondrial, Ribosomal, and Microsatellite DNA Markers

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