The polysaccharide fraction aip1 fromartemisia iwayomogi suppresses apoptotic death of the mouse spleen cells in culture

Abstract

A polysaccharide fraction, AIP1, purified from Artemisia iwayomogi was shown to have immunomodulating and anti-tumor activities in mice. In order to determine how the AIP1 fraction exhibits the immunomodulating activity, the effect of the fraction on the apoptosis of mouse spleen cells was investigated. Treatment of the mouse spleen cells with the AIP1 fraction resulted in the suppression of apoptotic death and an extension of cell survival in culture, indicating that the fraction might modulate the death of spleen cells. Treatment of the mice with the AIP1 fraction in vivo also resulted in less apoptosis of the spleen cells, which indicates the physiological relevance of the anti-apoptosis effect of the fraction in vitro. A mouse gene array was used to determine the profile of the gene expression change showing a pattern of up- and down-regulated genes by the AIP1 treatment. This study provides preliminary information regarding the immunomodulatory mechanism of the AIP1 fraction.

Polysaccharide biological response modifiers

“…The immunomodulatory mechanism of polysaccharide BRMs are not only restricted to mitogenic activation of immune cells but anti-apoptosis. Hwang et al demonstrated polysaccharide purified from Artemisia iwayomogi has the capability to suppress the apoptosis of mouse splenocytes both in vitro and in vivo [175]. Other than cell activation, polysaccharide BRMs trigger complement pathways of immune system.…”

Chemical Composition and Antibacterial Activity Against Oral Bacteria by the Essential Oil of Artemisia iwayomogi

“…The polysaccharide fraction AIP1 from A. iwayomogi suppresses apoptotic death of the mouse spleen cells in culture (11).…”

A Carbohydrate Fraction, AIP1, fromArtemisia IwayomogiReduces the Action Potential Duration by Activation of Rapidly Activating Delayed Rectifier K+Channels in Rabbit Ventricular Myocytes

“…Treatment of mouse thymocytes with AIP1 resulted in the suppression of cell death and improved cell survival, by suppression of the Fas/FasLdependent apoptotic pathway [12,13,19]. Furthermore, treatment of mouse spleen cells with the AIP1 fraction suppressed apoptosis via the modulation of apoptosis-related genes [11]. AIP1 has been shown to inhibit mast cell-derived immediate-type allergic reactions by lowering intracellular Ca…”

“…For example, the AIP1 fraction increased antibody production and suppressed transplanted tumor cell growth [10]. The AIP1 fraction may also be involved in the survival of immune cells, either by suppressing apoptosis or by stimulating cell proliferation [11][12][13]. To date, however, the influence of AIP1 on cardiac function has not been elucidated.…”