The Polypeptides of Hepatitis A Virus

Coulepis, A G; Locarnini, Stephen; Ferris, A. A.; Lehmann, Noreen I.; Gust, Ian D.

doi:10.1159/000148964

Cited by 43 publications

(44 citation statements)

References 8 publications

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“…wt. revealed in previous studies (Coulepis et al, 1978;Feinstone et al, 1979;Tratschin et al, i981) very likely represent aggregates of one or more structural polypeptides rather than contamination of virus samples with unrelated proteins.…”

Section: Discussionmentioning

confidence: 76%

The Physicochemical Properties of Infectious Hepatitis A Virions

Siegl

Frösner

Gauss‐Müller

et al. 1981

Journal of General Virology

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SUMMARYThe propagation of hepatitis A virus (HAV) in the cell line PLC/PRF/5 made possible the radiolabelling in vivo of mature, infectious hepatitis A virions and the determination of their physicochemical properties. In contrast to poliovirus type 2 (160S, 1.340 g/ml), HAV had a sedimentation coefficient of 156 + 2S and a buoyant density of 1.332 g/ml in CsCI. The genome of HAV consisted of linear single-stranded RNA which sedimented at 32.5S under non-denaturing conditions.

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Section: Discussionmentioning

confidence: 76%

The Physicochemical Properties of Infectious Hepatitis A Virions

Siegl

Frösner

Gauss‐Müller

et al. 1981

Journal of General Virology

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“…Coulcpis and coworkers [25] purified HAV from fecal specimens obtained from patients with naturally acquired hepatitis A by a pro cess of differential centrifugation, chloroform extraction, agarose gel filtration using Sepharose CL-2B. and isopycnic ultracentrifugation in CsCl density gradients.…”

Section: Fraction Numbermentioning

confidence: 99%

“…Pooled fractions were exam ined by IEM, and HAV was detected only in fractions where the SPRIA counts and the 260/280 nm ratios were high. (Reproduced from Conlepiset al [25] with permission.) system.…”

Section: Fraction Numbermentioning

confidence: 99%

Biophysical and Biochemical Characterization of Hepatitis A Virus

et al. 1982

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Biophysical and biochemical analysis of hepatitis A virus has shown it to be a 27- to 32-nm icosahedral particle with 32 capsomers. The mature virion has a buoyant density of 1.33–1.34 g/cm³, a sedimentation coefficient of 156–160S, and is composed of four polypeptides with molecular weights of 30,000–33,000 (VPl), 24,000–27,000 (VP2), 21,000–23,000 (VP3), and 7,000–14,000 (VP4). The genome of hepatitis A virus consists of a single piece of single-stranded RNA which sediments at 32–35S and has a buoyant density of 1.64 g/cm³. The molecular weight of RNA is 2.25 × 10⁶ when measured under nondenaturing conditions and 2.8 × 10⁶ when measured under fully denaturing conditions. The genome contains a 40–80 nucleotide sequence of polyadenylic acid and is capable of infecting cell cultures. These findings, together with the observation that the virion is stable at pH 3.0 and resistant to ether and a temperature of 60° for 1 h, indicate that hepatitis A virus should now be classified as an Entero-virus within the family Picornaviridae.

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“…Hepatitis A virus (HAV), a picornavirus, is probably an enterovirus in terms of its biophysical and biochemical characteristics (Siegl & Fr6sner, 1978a, b;Coulepis et al, 1978), but its biological behaviour, in some respects, is quite different. During the last 3 years, HAV has been propagated in several cell systems (including a human hepatoma cell line) without causing any apparent cytopathic effect and the incubation period is extraordinarily long for a picornavirus.…”

Section: Introductionmentioning

confidence: 99%

Persistent Infection of Human Fibroblasts by Hepatitis a Virus

Vallbracht¹,

Hofmann²,

Wurster

et al. 1984

Journal of General Virology

101

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SUMMARYInfection of human embryo fibroblasts with hepatitis A virus (HAV), a picornavirus, leads to an inapparent, persistent infection; cultures can be passed serially with consistent recovery of the virus in the supernatant. All of the cells of a HAV carrier culture are infected and proliferate. Subcultivation under HAV-immune serum cannot achieve a cure or even a reduction in the number of infected cells in HAV carrier cultures. No interferon activity can be detected during HAV infection and persistence. Addition of exogenous interferon eliminates HAV infection in vitro. Persistence of HAV in vitro appears to contradict the clinical course of HAV infection in vivo. The system presented offers the possibility of evaluating the role of immunological injury of HAV-infected cells, an injury which may lead to damage of these cells and to elimination of HAV during an HAV infection in vivo.

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The Polypeptides of Hepatitis A Virus

Cited by 43 publications

References 8 publications

The Physicochemical Properties of Infectious Hepatitis A Virions

The Physicochemical Properties of Infectious Hepatitis A Virions

Biophysical and Biochemical Characterization of Hepatitis A Virus

Persistent Infection of Human Fibroblasts by Hepatitis a Virus

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