The Polypeptide Structure of Transmissible Gastroenteritis Virus

Abstract

SUMMARYThe polypeptides of purified preparations of the coronavirus responsible for transmissible gastroenteritis of pigs have been examined by polyacrylamide gel electrophoresis. Four major polypeptides, VPI (mol. wt. 2ooooo), VP2 (5oooo), VP3 (3D ODD) and VP4 (28 5oo) and two minor polypeptides, VPIa 0o5ooo) and VP~b (80 5oo) have been reproducibly demonstrated in the virion, of which VPI, VP3 and VP4 contain carbohydrate. Treatment of the virion with the proteolytic enzyme bromelain removes the surface projections and VPI, thus identifying this glycopolypeptide as the major structural component of the projection.

Polypeptides of Infectious Bronchitis Virus. I. Polypeptides of the Virion

“…On comparing IBV polypeptides reported here and previously (Nagy & Lomniczi, 1979) to those of the porcine (Garwes & Pocock, 1975), murine (Sturman, 1977;Wege et al, 1979;Bond et al, 1979) and human (Hierholzer, 1976;Macnaughton, 1980) coronaviruses, a consistent coronavirus protein pattern is beginning to emerge. This is characterized by both large (tool.…”

“…In the last few years the molecular biology of coronaviruses has been extensively studied, and from studies on the protein structure of porcine (Garwes & Pocock, 1975;Pocock & Garwes, 1977), murine (Sturman, 1977, Sturman & Holmes, 1977Wege et al, 1979;Bond et aL, 1979) and human coronaviruses (Hierholzer, 1976;Macnaughton, 1980) a consistent pattern for coronavirus proteinS has begun to emerge. There is an unglycosylated nucleoprotein (mol.…”

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

“…TGEV virions contain three major structural proteins: the spike (S) glycoprotein, the integral membrane (M) glycoprotein, and the nucleocapsid (N) protein, which are the translation products from mRNAs 2, 6, and 7, respectively. The S glycoprotein forms the peplomers on the virion envelop and induces neutralizing antibodies [9,10,14,20]. Unlike other coronaviruses, the S glycoprotein of TGEV, feline infectious peritonitis virus and canine coronavirus is not cleaved into two proteins [11,18,19].…”

Identification of a 17000 Molecular Weight Antigenic Polypeptide in Transmissible Gastroenteritis Virus-infected Cells

“…In the case of TGEV, these proteins appear as three major bands on SDS-polyacrylamide gels with apparent molecular weights of 200 000 (200K), 47K to 56K and 30K (Garwes & Pocock, 1975;Brian et al, 1984;Hu et al, 1984). The 47K to 56K phosphorylated nucleocapsid protein is associated with the non-segmented, positive-stranded RNA genome.…”