The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis

Itoh, Naoto; Yonehara, Shin; Ishii, Ayano; Yonehara, Minako; Mizushima, Sei Ichi; Sameshima, Masazumi; Hase, Atsushi; Seto, Yoshiyuki; Nagata, Shinji

doi:10.1016/0092-8674(91)90614-5

Cited by 2,612 publications

(1,398 citation statements)

References 36 publications

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“…All except one sample of normal mucosa examined expressed the 2.7-and 1.9-kb Fas mRNA bands previously reported by Northern analysis (Itoh et al, 1991 …”

Section: Expression Of Fas Mrna In Colon Tumoursmentioning

confidence: 51%

“…Fas protein is expressed in variable levels on the surface of several colon cancer cell lines. In some cell lines, low expression of Fas may be up-regulated by the cytokines TNF-a or interferon gamma (IFN-y) to levels similar to that of activated T cells (Yonehara et al, 1989;Itoh et al, 1991;Moller et al, 1994 (Yonehara et al, 1989;Abreu-Martin et al, 1995), while growth of KM12C cells is inhibited by the anti-Fas antibody (Owen-Schaub et al, 1993, 1994. Metastatic variants of KM12C cells are more resistant to the effects of the antibody, despite a similar level of Fas expression (Owen-Schaub et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…The Fas antigen (also called APO-1 or CD95) is a cell surface receptor, homologous to members of the tumour necrosis factor (TNF) family of transmembrane proteins (Itoh et al, 1991). Ligation of Fas on the cell surface, by its endogenous ligand or by agonistic antibodies, triggers rapid apoptosis of the cell (Trauth et al, 1989;Yonehara et al, 1989).…”

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confidence: 99%

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Down-regulation of Fas gene expression in colon cancer is not a result of allelic loss or gene rearrangement

Butler

Hewett²,

Butler³

et al. 1998

Br J Cancer

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Summary Expression of Fas, an apoptosis-inducing receptor, in colonic epithelium is progressively reduced during malignant transformation. We have examined the human Fas gene for loss of heterozygosity (LOH) and gross rearrangements in colon tumours and matched normal mucosa. Polymerase chain reaction (PCR) primers were designed to span a Dral restriction fragment length polymorphic site in the gene. Heterozygosity was detected in normal DNA samples by PCR amplification of the polymorphic site and restriction enzyme digestion. Thirtyeight of 88 patients (43%) with colon carcinomas were informative for the assay, and LOH was detected in 6 of the 38 (16%) corresponding tumours. Tumours from three patients with LOH did not express detectable Fas mRNA, and Fas expression was reduced or absent in 7 of 11 tumours from informative patients without LOH. Southern blotting of tumour DNA samples was used to detect rearrangement of the Fas gene, but no altered hybridization patterns were observed in 64 tumours analysed. These findings indicate that disruption of the Fas gene is not primarily responsible for the loss of Fas protein expression reported in colon cancer. We have also shown that loss of Fas gene transcription is common in these tumours, which may be due to epigenetic gene silencing.

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“…All except one sample of normal mucosa examined expressed the 2.7-and 1.9-kb Fas mRNA bands previously reported by Northern analysis (Itoh et al, 1991 …”

Section: Expression Of Fas Mrna In Colon Tumoursmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Down-regulation of Fas gene expression in colon cancer is not a result of allelic loss or gene rearrangement

Butler

Hewett²,

Butler³

et al. 1998

Br J Cancer

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“…We used a¯uorescent substrate for the sub-group of caspases 3, 7 and 10 to measure caspase activity following Fas triggering. Figure 1b (Itoh et al, 1991) to require cycloheximide to deliver an apoptotic signal. In the experiments of Figure 1, 1% WEHI-3B conditioned medium was used as source of IL-3.…”

Section: Resultsmentioning

confidence: 99%

“…Fas-induced apoptosis is critical for the deletion of autoreactive T lymphocytes, for cytotoxic T lymphocyte cytolysis and for lymphocyte elimination in sites of immune privilege (Nagata, 1997). Fas and TNFR1 share a region of homology in the cytoplasmic tail, the death domain, which is critical for signalling apoptosis (Itoh et al, 1991;Tartaglia et al, 1993). The death domain recruits FADD upon Fas activation (Kischkel et al, 1995), this then recruits and activates caspase 8 (Muzio et al, 1996;Boldin et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

JNK activation is not required for Fas-mediated apoptosis

et al. 1999

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Fas ligation in the presence of cycloheximide induced Jun N-terminal kinase 1 (JNK1) and JNK2 phosphorylation, caspase activation and cell death in the IL-3-dependent cell line BAF3. Fas-mediated apoptosis was prevented by expression of dominant negative FADD but not inhibited by IL-3. To investigate the role of JNK activation in this process, we examined cells overexpressing a JNK-speci®c phosphatase M3/6. M3/6 prevented Fas stimulation of JNK, but did not a ect Fas-mediated caspase activation or cell death, demonstrating that JNK activation is not required for these processes.

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Maldistribution of Interstitial Neurons in Prefrontal White Matter of the Brains of Schizophrenic Patients

Akbarian

Jj²,

Potkin³

et al. 1996

Arch Gen Psychiatry

366

212

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The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis

Cited by 2,612 publications

References 36 publications

Down-regulation of Fas gene expression in colon cancer is not a result of allelic loss or gene rearrangement

Down-regulation of Fas gene expression in colon cancer is not a result of allelic loss or gene rearrangement

JNK activation is not required for Fas-mediated apoptosis

Maldistribution of Interstitial Neurons in Prefrontal White Matter of the Brains of Schizophrenic Patients

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