The polymorphism of chicken-type lysozyme gene in Japanese flounder ( Paralichthys olivaceus ) and its association with resistance/susceptibility to Listonella anguillarum

Liu, Jinxiang; Zhou, Nayu; Fu, Ruyan; Cao, Dandan; Shi, Yu; Li, Aoyun; Zhao, Haitao; Zhang, Quanqi; Yu, Haiyang

doi:10.1016/j.fsi.2017.05.010

Cited by 17 publications

(5 citation statements)

References 48 publications

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“…Penaeidin's C-terminal cysteine-rich domain with its amphipathic shape may perform as the domain for the binding pathogen. Lysozyme is another important AMP that is involved in the host-resistance arrangement of invading microorganisms [75][76][77]. In a previous study with blue shrimp (Litopenaeus stylirostris), lysozyme was found to be upregulated in WSSV-infected shrimp, suggesting its involvement in the innate immune response of shrimp to WSSV [78].…”

Section: Discussionmentioning

confidence: 99%

Circulating Phylotypes of White Spot Syndrome Virus in Bangladesh and Their Virulence

et al. 2022

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White Spot Syndrome Virus (WSSV) has emerged as one of the most prevalent and lethal viruses globally and infects both shrimps and crabs in the aquatic environment. This study aimed to investigate the occurrence of WSSV in different ghers of Bangladesh and the virulence of the circulating phylotypes. We collected 360 shrimp (Penaeus monodon) and 120 crab (Scylla sp.) samples from the south-east (Cox’s Bazar) and south-west (Satkhira) coastal regions of Bangladesh. The VP28 gene-specific PCR assays and sequencing revealed statistically significant (p < 0.05, Kruskal–Wallis test) differences in the prevalence of WSSV in shrimps and crabs between the study areas (Cox’s Bazar and Satkhira) and over the study periods (2017–2019). The mean Log load of WSSV varied from 8.40 (Cox’s Bazar) to 10.48 (Satkhira) per gram of tissue. The mean values for salinity, dissolved oxygen, temperature and pH were 14.71 ± 0.76 ppt, 3.7 ± 0.1 ppm, 34.11 ± 0.38 °C and 8.23 ± 0.38, respectively, in the WSSV-positive ghers. The VP28 gene-based phylogenetic analysis showed an amino-acid substitution (E→G) at the 167th position in the isolates from Cox’s Bazar (referred to as phylotype BD2) compared to the globally circulating one (BD1). Shrimp PL artificially challenged with BD1 and BD2 phylotypes with filtrates of tissue containing 0.423 × 109 copies of WSSV per mL resulted in a median LT50 value of 73 h and 75 h, respectively. The in vivo trial showed higher mean Log WSSV copies (6.47 ± 2.07 per mg tissue) in BD1-challenged shrimp PL compared to BD2 (4.75 ± 0.35 per mg tissue). Crabs infected with BD1 and BD2 showed 100% mortality within 48 h and 62 h of challenge, respectively, with mean Log WSSV copies of 12.06 ± 0.48 and 9.95 ± 0.37 per gram tissue, respectively. Moreover, shrimp antimicrobial peptides (AMPs), penaeidin and lysozyme expression were lower in the BD1-challenged group compared to BD2 challenged shrimps. These results collectively demonstrated that relative virulence properties of WSSV based on mortality rate, viral load and expression of host immune genes in artificially infected shrimp PL could be affected by single aa substitution in VP28.

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Section: Discussionmentioning

confidence: 99%

Circulating Phylotypes of White Spot Syndrome Virus in Bangladesh and Their Virulence

et al. 2022

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“…Two SNPs in exon 2 (position 655, CC) and intron 3 (position 934, CT) of the LYSC gene reported being correlated with the big-belly disease resistance in Asian sea bass (Fu et al 2013). The genotype CC at position 536, CC at position 1200, and GG at position 140 in the Japanese flounder LYSC were reported to be correlated with the resistance to Listonela anguillarum (Liu et al 2017). For future development, it is essential to examine and confirm the association of the SNP2 in a larger population before its application as the potential marker in the African catfish selective breeding program.…”

Section: The Mrna Expression and Snp Associations To The Aeromonas Hymentioning

confidence: 98%

“…LYSC polymorphism also found in other fish species. Japanese flounder Paralichthys olivaceus has eight polymorphism sites in its LYSC promotor and nine SNPs in the coding region (Liu et al 2017). Asian seabass has two SNPs in the exon and the intron of the LYSC coding sequence (Fu et al 2013).…”

Section: Genomic Structure and Mrna Expressionmentioning

confidence: 99%

“…Both lysozymes gene has been demonstrated to play essential roles as the immune defense in many fish species (Mohanty dan Sahoo 2010;Pridgeon et al 2013;Wei et al 2014;Di Falco et al 2017). The involvement of the LYSC in the protection against bacterial infection has been reported in fish species, such as resistant families of Atlantic salmon against Piscirickettsia salmonis infection (Dettleff et al 2015), channel catfish against Edwardsiella ictaluri (Wang et al 2013), Darby's sturgeon and Qihe crucian carp against A. hydrophila (Wang et al 2016;Zhang et al 2018), Moreover, the single nucleotide polymorphisms (SNPs) in LYSC gene have been characterized in several fish species, such as Asian seabass (Fu et al 2013) and Japanese flounder (Liu et al 2017) and identified as potential markers for bacterial-resistance in fish. However, such studies in African catfish are still limited.…”

Section: Introductionmentioning

confidence: 99%

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Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

et al. 2019

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Abstract. Authors. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrated to play important roles in the fish protection system against bacterial infection. In this present study, we aimed to identify the single nucleotide polymorphism (SNP) within the LYSC gene of African catfish Clarias gariepinus and its possible association with Aeromonas hydrophila resistance, a major pathogenic bacterium in African catfish. The gDNA of the African catfish LYSC coding sequence was 1559 bp long, comprising of four exons and three introns. Six SNPs were identified in African catfish LYSC, namely SNP1-6. After the A. hydrophila challenge, we regarded the surviving individuals after the infection as the resistant group and the dead fish as the susceptible group. High-resolution melting (HRM) analysis on SNP2 revealed that the allele frequencies of TT, CC, and TC were of 27.78%, 5.55%, and 66.67% in the resistant group, while the frequencies of TT, CC, and TC were 16.67%, 27.78%, and 55.55% in the susceptible group, respectively. The expression of LYSC and other immune-genes in the resistant group was also higher in the liver, head kidney, and spleen. These results indicated that the LYSC gene might play an essential role in bacterial resistance, and the SNP2 within the LYSC gene may be associated with the resistance to A. hydrophila in African catfish.

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“…ALP is a phosphomonoesterase that decomposes phosphomonoesters, provides phosphate groups for the body, regulates membrane transport, participates in the metabolism of DNA, RNA, lipids and proteins, and has an important impact on the body's immunity (Chen et al, 1998). While, lysozyme is an alkaline enzyme that decomposes the insoluble mucopolysaccharides into soluble glycopeptides by destroying the cell wall of bacteria, which leads to the rupture of cell wall and the release of cell wall contents to cause the dissolution of bacteria (Liu et al, 2017;Ma et al, 2020), hydrolyze mucopolysaccharide from pathogenic bacteria, which has antibacterial, antiinflammatory and antiviral effects.…”

Section: Growth Performance Feed Efficiency and Survival Ratementioning

confidence: 99%

Chitosan Nanoparticles as Nutritional Stimulus in the Early Feeding of the Nile Tilapia Fry (Oreochromis niloticus)

Aboseif et al.

2024

Egypt. J. of Aquatic Biolo. and Fish.

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INTRODUCTIONThroughout the past few decades, the emergence of new scientific challenges to improve fish nutrition and the rapid development of aquaculture production has been observed. Moreover, fish nutrition is vital for the development of a sustainable aquaculture (FAO, 2018). The diet formulations used for farmed fish have been largely modified in the past few years. However, bottlenecks still exist in being able to suppress

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The polymorphism of chicken-type lysozyme gene in Japanese flounder ( Paralichthys olivaceus ) and its association with resistance/susceptibility to Listonella anguillarum

Cited by 17 publications

References 48 publications

Circulating Phylotypes of White Spot Syndrome Virus in Bangladesh and Their Virulence

Circulating Phylotypes of White Spot Syndrome Virus in Bangladesh and Their Virulence

Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

Chitosan Nanoparticles as Nutritional Stimulus in the Early Feeding of the Nile Tilapia Fry (Oreochromis niloticus)

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