The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p

Beggs, Suzanne; James, Tharappel C.; Bond, Ursula

doi:10.1093/nar/gkr1108

Cited by 23 publications

(14 citation statements)

References 35 publications

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“…Polyadenylation of histone read-through transcripts might therefore be a signal to trigger their degradation. In this context, it is interesting that in budding yeast, the TRAMP-exosome complex regulates histone metabolism (35). Moreover, it has recently been shown that polyadenylation induces exosome-mediated decay of promoter upstream transcripts (PROMPTs) to control promoter directionality (42,43).…”

Section: Discussionmentioning

confidence: 99%

CstF64: Cell Cycle Regulation and Functional Role in 3′ End Processing of Replication-Dependent Histone mRNAs

Romeo

Griesbach

Schümperli

2014

Molecular and Cellular Biology

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The 3= end processing of animal replication-dependent histone mRNAs is activated during G 1 /S-phase transition. The processing site is recognized by stem-loop binding protein and the U7 snRNP, but cleavage additionally requires a heat-labile factor (HLF), composed of cleavage/polyadenylation specificity factor, symplekin, and cleavage stimulation factor 64 (CstF64). Although HLF has been shown to be cell cycle regulated, the mechanism of this regulation is unknown. Here we show that levels of CstF64 increase toward the S phase and its depletion affects histone RNA processing, S-phase progression, and cell proliferation. Moreover, analyses of the interactions between CstF64, symplekin, and the U7 snRNP-associated proteins FLASH and Lsm11 indicate that CstF64 is important for recruiting HLF to histone precursor mRNA (pre-mRNA)-resident proteins. Thus, CstF64 is central to the function of HLF and appears to be at least partly responsible for its cell cycle regulation. Additionally, we show that misprocessed histone transcripts generated upon CstF64 depletion mainly accumulate in the nucleus, where they are targets of the exosome machinery, while a small cytoplasmic fraction is partly associated with polysomes.

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Section: Discussionmentioning

confidence: 99%

CstF64: Cell Cycle Regulation and Functional Role in 3′ End Processing of Replication-Dependent Histone mRNAs

Romeo

Griesbach

Schümperli

2014

Molecular and Cellular Biology

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“…The fact that the correct dependence of histone transcript concentration on cell volume does not require direct feedback suggests that instead it is an intrinsic property of either transcription rate or mRNA degradation. To test if degradation from the 3'-end by the nuclear exosome is required, we analyzed the cell-volume-dependence of histone transcript concentrations in strains where we deleted RRP6, a component of the nuclear exosome exonuclease 32,33 . As shown in Fig.…”

Section: '-To-5'-degradation By the Nuclear Exosome Is Not Necessarymentioning

confidence: 99%

Transcription coordinates histone amounts and genome content

Claude

Bureik

Adarska

et al. 2020

Preprint

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Biochemical reactions typically depend on the concentrations of the molecules involved, and cell survival therefore critically depends on the concentration of proteins. To maintain constant protein concentrations during cell growth, global mRNA and protein synthesis rates are tightly linked to cell volume. While such regulation is appropriate for most proteins, certain cellular structures do not scale with cell volume. The most striking example of this is the genomic DNA, which doubles during the cell cycle and increases with ploidy, but is independent of cell volume.Here, we show that the amount of histone proteins is coupled to the DNA content, even though mRNA and protein synthesis globally increase with cell volume. As a consequence, and in contrast to the global trend, histone concentrations (i.e. amounts per volume) decrease with cell volume but increase with ploidy. We find that this distinct coordination of histone homeostasis and genome content is already achieved at the transcript level, and is an intrinsic property of histone promoters that does not require direct feedback mechanisms. Mathematical modelling and histone promoter truncations reveal a simple and generalizable mechanism to control the cell volume- and ploidy-dependence of a given gene through the balance of the initiation and elongation rates.

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“…This promiscuity implies that there is plasticity in termination options and that there may be an ongoing competition between termination machineries for elongation complexes. Sen1 has also been observed to act both outside NNS, where it participates in processing or termination events at the end of pre-mRNA transcription units (27, 91), and in the resolution of RNA–DNA hybrids (92, 93). …”

Section: Recruitment Of the Nrd1–nab3–sen1 Complex To Its Targetsmentioning

confidence: 99%

Termination of Transcription of Short Noncoding RNAs by RNA Polymerase II

Arndt

Reines

2015

Annu. Rev. Biochem.

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The RNA polymerase II transcription cycle is often divided into three major stages: initiation, elongation, and termination. Research over the last decade has blurred these divisions and emphasized the tightly regulated transitions that occur as RNA polymerase II synthesizes a transcript from start to finish. Transcription termination, the process that marks the end of transcription elongation, is regulated by proteins that interact with the polymerase, nascent transcript, and/or chromatin template. The failure to terminate transcription can cause accumulation of aberrant transcripts and interfere with transcription at downstream genes. Here, we review the mechanism, regulation, and physiological impact of a termination pathway that targets small noncoding transcripts produced by RNA polymerase II. We emphasize the Nrd1–Nab3–Sen1 pathway in yeast, in which the process has been extensively studied. The importance of understanding small RNA termination pathways is underscored by the need to control noncoding transcription in eukaryotic genomes.

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The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p

Cited by 23 publications

References 35 publications

CstF64: Cell Cycle Regulation and Functional Role in 3′ End Processing of Replication-Dependent Histone mRNAs

CstF64: Cell Cycle Regulation and Functional Role in 3′ End Processing of Replication-Dependent Histone mRNAs

Transcription coordinates histone amounts and genome content

Termination of Transcription of Short Noncoding RNAs by RNA Polymerase II

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