The poly(phenolic) domain of potato suberin: a non-lignin cell wall bio-polymer

Bernards, Mark A.; Razem, Fawzi A.

doi:10.1016/s0031-9422(01)00046-2

Cited by 127 publications

(130 citation statements)

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“…The 13 C CPMAS NMR and FT-IR spectra of these residues (Figures 4 and 5) confirm the results obtained from the other analyses and from previous observations on similar residues (9, 10). The spectra of residue 1 ( Figure 4A (6,7,9,10).…”

Section: Resultssupporting

confidence: 90%

“…Epoxysubstituted monomers were absent in cutinase hydrolysis products, just as they were in the milder NaOMe methanolysis. The residue from the enzymatic hydrolysis (residue 2, Figure 1) was also subjected to chemical depolymerization to solubilize the remaining ester-bound suberin monomers, which were shown to exist in the product by FT-IR and solid state 13 C NMR (see later in the text). This reaction yielded monomer fraction 4 (Figure 1), which contained more ω-hydroxy acids compared to R,ω-diacids and more unidentified compounds than the other methods ( Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…Additional information on the mode of action of the cutinase CcCUT1 compared with chemical depolymerization can be achieved from the solid residues obtained from the reactions. Due to their insoluble nature, the residues from chemical depolymerization (residue 1, Figure 1) and cutinase hydrolysis (residue 2, Figure 1) were analyzed by solid state 13 C CPMAS NMR and by FT-IR. Both techniques have previously been used successfully, for example, in cork and potato suberin analysis (4, 6-10), one study tackling, in particular, the effects of suberin removal (10).…”

Section: Resultsmentioning

confidence: 99%

“…Noncellulosic residues from this reaction represented about 50 and 70% of the original mass of the cutinase hydrolysis residue and the chemical depolymerization residue, respectively. These residues were analyzed with solid state 13 C CP MAS NMR techniques and a SD, standard deviation; -, not detected; ns, not separated from unidentified peak; tr, <0.1%. Number in parentheses after the compound name refers to peak identifications in Table 2 and numbers for major peaks in Table 3.…”

Section: Resultsmentioning

confidence: 99%

“…Residues after chemical depolymerization (residue 1, Figure 1) and cutinase hydrolysis (residue 2, Figure 1) were subjected to a second cellulase treatment in an attempt to remove the remaining, evidently originally shielded, cellulosic material, which was observed in the FT-IR and solid state 13 C NMR experiments (see Results and Discussion). An aliquot of residue 1 served as a control, without the enzyme in the mixture.…”

Section: Methodsmentioning

confidence: 99%

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Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

Järvinen¹,

Silvestre²,

Holopainen³

et al. 2009

J. Agric. Food Chem.

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Chemical and enzymatic depolymerizations of suberin isolated from potato peel (Solanum tuberosum var. Nikola) were performed under various conditions. Enzymatic hydrolysis with cutinase CcCut1 and chemical methanolysis with NaOMe of suberin yielded monomeric fragments, which were identified as TMS derivatives with GC-MS and GC-FID. The solid, hydrolysis-resistant residues were analyzed with solid state 13 C CPMAS NMR, FT-IR, and microscopic methods. Methanolysis released more CHCl 3 -soluble material than the cutinase treatment when determined gravimetrically. Interestingly, cutinase-catalyzed hydrolysis produced higher proportions of aliphatic monomers than hydrolysis with the NaOMe procedure when analyzed by GC in the form of TMS derivatives. Monomers released by the two methods were mainly R,ω-dioic acids and ω-hydroxy acids, but the ratios of the detected monomers were different, at 40.0 and 32.7% for methanolysis and 64.6 and 8.2% for cutinase, respectively. Thus, cutinase CcCut1 showed higher activity toward ester bonds of R,ω-dioic acids than toward the bonds of ω-hydroxy acids. The most abundant monomeric compounds were octadec-9-ene-1,18-dioic acid and 18-hydroxyoctadec-9-enoic acid, which accounted for ca. 37 and 28% of all monomers, respectively. The results of the analyses of the chemical and enzymatic hydrolysis products were supported by the spectroscopic analyses with FT-IR and CPMAS 13 C NMR together with the analysis of the microstructures of the hydrolysis residues by light and confocal microscopy.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

Järvinen¹,

Silvestre²,

Holopainen³

et al. 2009

J. Agric. Food Chem.

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Biopolymers from Plants

Sabater

Ródenas

Heredia

2013

Handbook of Biopolymer‐Based Materials

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Neither lysigenous nor just oil: Demystifying myrtaceous secretory cavities

Richit,

Díaz,

Dick

et al. 2023

American J of Botany

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PremiseLeaf subepidermal secretory cavities are a notable trait in Myrtaceae. The formation of these secretory structures is still controversial because of the lack of consensus on their ontogeny among authors. The knowledge about the nature of the compounds present in these cavities has grown over the last few years, demonstrating that terpenoid‐rich oils are not their unique content. These two points are the focus of this study, which investigates the ontogeny, structure and the contents of secretory cavities in Neotropical Myrtaceae.MethodsWe used histochemical tests and Raman analysis to verify the basic chemical composition of cavity content of nine species and investigated the ontogeny of glands in one species, comparing aldehyde‐fixed tissues and fresh sections mounted in an inert medium.ResultsWe observed schizogenous development and appearance in secretory cavities and found that tissue processing may induce cell breakdown, which can be misinterpreted as lysigeny. The content of these cavities contains prospective terpenes, resins, carbonyl groups, and flavonoids.ConclusionsOur findings support the hypothesis that technical artifacts may produce a lysigenous impression in oil glands. These tissue distortions must be considered when interpreting the development of this type of secretory structure. Moreover, the basic analyses of chemical constituents show for the first time that the glands of Neotropical Myrtaceae are potential reservoirs of some compounds previously reported as novelties for a few other myrtaceous species, such as flavonoids. As some of them are non‐lipid compounds, the idea that glands are just oil repositories is no longer applicable.This article is protected by copyright. All rights reserved.

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The poly(phenolic) domain of potato suberin: a non-lignin cell wall bio-polymer

Cited by 127 publications

References 55 publications

Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

Biopolymers from Plants

Neither lysigenous nor just oil: Demystifying myrtaceous secretory cavities

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