The poly(A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase

Nag, Anita; Narsinh, Kazim; Martinson, Harold G.

doi:10.1038/nsmb1253

Cited by 67 publications

(89 citation statements)

References 60 publications

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“…Mean values from n PCR reactions with standard errors of mean (SEM) are shown. This model is based on data reported here and in references 30,31 .…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…CPSF can bind to TFIID and to the body of pol II suggesting the hypothesis that CPSF binds first to the promoter via TFIID and is then transferred to pol II 30 , 31. During elongation, CPSF is proposed to associate with pol II in a manner that excludes CstF and later, CstF is recruited, at a pause site following transcription of the AAUAAA element 30,32 . Pausing has been detected downstream of the β-actin gene where it has been linked to transcription termination 33 .…”

Section: Introductionmentioning

confidence: 99%

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RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes

Glover-Cutter

Kim

Espinosa

et al. 2007

Nat Struct Mol Biol

290

329

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We investigated co-transcriptional recruitment of pre-mRNA processing factors to human genes. Capping factors associate with paused RNA pol II at the 5′ ends of quiescent genes. They also track throughout actively transcribed genes, and accumulate with paused polymerase in the 3′ flanking region. 3′ processing factors CstF and CPSF are maximally recruited 0.5-1.5 kb downstream of poly (A) sites where they coincide with capping factors, Spt5, and Ser2 hyperphosphorylated, paused pol II. 3′ end processing factors also localize at transcription start sites, and this early recruitment is enhanced after polymerase arrest with DRB. These results suggest that promoters may help specify recruitment of 3′ end processing factors. We propose a dual pausing model where elongation arrests near the transcription start site and in the 3′ flank to allow co-transcriptional processing by factors recruited to the pol II ternary complex.

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“…Mean values from n PCR reactions with standard errors of mean (SEM) are shown. This model is based on data reported here and in references 30,31 .…”

Section: Supplementary Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes

Glover-Cutter

Kim

Espinosa

et al. 2007

Nat Struct Mol Biol

290

329

View full text Add to dashboard Cite

show abstract

“…It has been suggested that the poly(A) signal itself can direct RNAPII pausing. The AAUAAA-dependent pausing is mediated by CPSF but does not require the CTD and any other downstream pausing elements (Orozco et al 2002;Park et al 2004;Nag et al 2006Nag et al , 2007. Another sequence, a CCAAT-box in the adenovirus late promoter is necessary for termination of the upstream gene (Connelly and Manley 1989).…”

Section: Pausing the Polymerase: Another Factor In Rnapii Terminationmentioning

confidence: 99%

Transcription termination by nuclear RNA polymerases

Richard

Manley²

2009

Genes Dev.

285

325

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Gene transcription in the cell nucleus is a complex and highly regulated process. Transcription in eukaryotes requires three distinct RNA polymerases, each of which employs its own mechanisms for initiation, elongation, and termination. Termination mechanisms vary considerably, ranging from relatively simple to exceptionally complex. In this review, we describe the present state of knowledge on how each of the three RNA polymerases terminates and how mechanisms are conserved, or vary, from yeast to human.

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“…However, we cannot exclude that for some other genes, or in addition to nuclear effects, DOXO might also affect cytoplasmic mRNA isoform stability, for example through miRNAs. As cleavage/polyadenylation is connected to transcription termination, and polyA sites induce RNA polymerase II (Pol-II) pausing 33 , we determined the impact of DOXO and CPT on Pol-II dynamics on the CENPN gene by chromatin immunoprecipitation. In untreated cells, we observed peaks of Pol-II levels around both E12 and E13 polyA sites compared with matched upstream regions (Fig.…”

Section: Doxo-regulated Ales Play a Role In Cell Cycle Regulationmentioning

confidence: 99%

A recently evolved class of alternative 3′-terminal exons involved in cell cycle regulation by topoisomerase inhibitors

et al. 2014

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Alternative 3 0 -terminal exons, which use intronic polyadenylation sites, are generally less conserved and expressed at lower levels than the last exon of genes. Here we discover a class of human genes, in which the last exon appeared recently during evolution, and the major gene product uses an alternative 3 0 -terminal exon corresponding to the ancestral last exon of the gene. This novel class of alternative 3 0 -terminal exons are downregulated on a large scale by doxorubicin, a cytostatic drug targeting topoisomerase II, and play a role in cell cycle regulation, including centromere-kinetochore assembly. The RNA-binding protein HuR/ ELAVL1 is a major regulator of this specific set of alternative 3 0 -terminal exons. HuR binding to the alternative 3 0 -terminal exon in the pre-messenger RNA promotes its splicing, and is reduced by topoisomerase inhibitors. These findings provide new insights into the evolution, function and molecular regulation of alternative 3 0 -terminal exons.

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The poly(A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase

Cited by 67 publications

References 60 publications

RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes

RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes

Transcription termination by nuclear RNA polymerases

A recently evolved class of alternative 3′-terminal exons involved in cell cycle regulation by topoisomerase inhibitors

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