The polarographic estimation of steroid hormones

The Excretion of 17-Kestoteroids in Men of Different Age-Groups, with Special Reference to Prostatic cancer

“…Fewer cases were investigated in this lower age-group, but the results obtained are in general agreement with those found by other workers (Barnet, Henly, Morris and Warren, 1946), and they are included in the summaries of all the results given in Table I …”

“…In the hands of many different workers (Barnett, Henly, Morris and Warren, 1946) this method has revealed a very wide range of excretion of 17-ketosteroids by normal men, at least in the lower age-groups. An adult male may excrete in a range which is at least 7-28 mg./day.…”

17-Ketosteroid Excretion in Men with Gout

“…However, Wolfson (1954) has criticized the polarographic method employed by the latter authors, on the basis that "it is not a valid method of estimating 1 7-ketosteroid excretion as shown by its failure to show low values in gout patients". It is probable, however, that the technique is more specific than the many modifications of the colorimetric Zimmerman reaction (Barnett, Henly, and Morris, 1946). Barnett, Henly, Morris, and Warren (1946) and Butt, Morris, Robinson, and Warren (1951) have shown that in normal subjects the colorimetric method may give somewhat higher results than the polarographic technique.…”

Biochemistry of the Hormones

“…On the other hand, Bender & Wilson (124) , who investigated and rejected the method of Talbot et al (125), believe that no satisfactory chemical method is avail able for urinary estrogens, especially when small amounts are present; they suggest that bioassay is the only reliable method. The polarographic determination of several steroids has been studied by Sartori & Bianchi (126) , Bjornson et al (127) and by Barnett and co-workers (128,129,130) . Johnston (131) has tested the chromatographic separation of steroid dinitrophenylhydra zones.…”