The polarographic determination of dimethylnitrosamine in animal tissue

Heath, D. F.; Jarvis, J. A. E.

doi:10.1039/an9558000613

Cited by 61 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carcinogenic N-nitrosamines are known to be formed from secondary amines and nitrite in microbial cultures (11). At least 10% of the strains ofEscherichia coli, Clostridium, Bacteroides, Bifidobacterium, and enterococci tested by Hawksworth and Hill (6,7; G. Hawksworth and M. Hill, Biochem. J.…”

mentioning

confidence: 99%

N-Nitrosamine formation by cultures of several microorganisms

Mills¹,

Alexander²

1976

Appl Environ Microbiol

View full text Add to dashboard Cite

Of 38 pure cultures of microorganisms tested, only one, Pseudomonas stutzeri, was capable of forming dimethylnitrosamine from dimethylamine and nitrite during growth. Resting cells of P. stutzeri, Cryptococcus terreus, Escherichia coli, and Xanthomonas campestris formed dimethylnitrosamine, although no nitrosamine was found in growing cultures of the latter three organisms. No nitrosamine was produced by either growing cultures or resting-cell suspensions of Pseudomonas fragi or Proteus mirabilis. Boiled cells of P. stutzeri, but not those of C. terreus, E. coli, and X. campestris, formed dimethylnitrosamine, and this nitrosamine was also produced by extracts of E. coli cells at pH 5.0.

show abstract

mentioning

confidence: 99%

N-Nitrosamine formation by cultures of several microorganisms

Mills¹,

Alexander²

1976

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The polarographic behavior of some N-nitrosamines has been investigated [12][13][14][15][16][17] and pulse polarography has been found to be a very sensitive technique for N-nitrosamine determinations. Although its rather poor selectivity makes impossible the specific determination of one type of N-nitrosamine in the presence of another one without a prior separation step, polarography, however, has a number of advantages: it can be applied to both volatile and nonvolatile compounds, it provides a rapid analysis with simple and inexpensive apparatus and it does not require sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Reduction of Prilocaine as itsN-Nitrosamine Derivative at the Mercury Electrode

et al. 1998

View full text Add to dashboard Cite

The polarographic behavior of prilocaine as its N-nitrosamine derivative, was studied using several electrochemical techniques. In acidic media, a nonreversible diffusion controlled reduction process involving two electrons takes place (E 1=2 ¼ ¹0:86 V at pH 3.5). A differential pulse polarographic method is described for determining prilocaine based on the formation of its N-nitrosamine derivative in aqueous solution. The method has been validated for a series of artificial matrices where different functional groups are present. It was also applied to a pharmaceutical formulation.

show abstract

“…The controls had oil alone added to their diet except for one group of 6 male and 6 female included from another experiment which were fed pellets. At intervals during the earlier experiments the level of DMN in the diets was checked by the method of Heath and Jarvis (1955). The amount of DMN was always within 20% of the expected concentration and it did not fall off within the periods (1-2 weeks) during which the batches of prepared diets were used.…”

Section: Methodsmentioning

confidence: 99%

Hepatic pathology in rats on low dietary levels of dimethylnitrosamine.

Terracini¹,

Magee²,

Barnes³

1967

Br J Cancer

122

View full text Add to dashboard Cite

DIMETHYLNITROSAMINE (DMN) is a very powerful liver carcinogen for the rat (Magee and Barnes, 1956) and will produce malignant tumours at other sites and in other species (Magee and Barnes, 1967).Because of the possible importance of certain nitrosamines as aetiological agents for cancer in the human environment (Magee and Barnes, 1967) it was important that an attempt should be made to try to obtain experimental data on the basis of which it might be possible to calculate a non-effective dose of this compound. This also required an evaluation of non-neoplastic lesions appearing in animals exposed to the carcinogenic nitrosamine and their relation to tumour development. The experiments reported here were carried out in this laboratory since 1957 and dietary concentrations of DMN ranging between 2 and 50 p.p.m. were used. No non-neoplastic lesions were found to be specifically linked with the appearance of tumours. The findings are discussed in relation to the problem of constructing the dose-response relationship of a powerful carcinogen which in this case is probably produced within the animal body from the compound which is added to the diet. MATERIALS AND METHODSMale and female white rats (Porton strain) 4-6 weeks old were housed 6 to a cage. All rats were given Diet 41B (Bruce and Parkes, 1957) in powder form. Food intake was measured throughout the experiments and the rats were weighed twice weekly. All sick animals and any animal found to be losing weight were killed with coal-gas. DMN (British Drug Houses, Ltd.) was added to the powdered diet as a solution in arachis oil. The solutions in oil were so made up that oil was added at the rate of 10 ml. to each kilo of powdered diet and mixed in a Hobart food mixer. The controls had oil alone added to their diet except for one group of 6 male and 6 female included from another experiment which were fed pellets. At intervals during the earlier experiments the level of DMN in the diets was checked by the method of Heath and Jarvis (1955). The amount of DMN was always within 20% of the expected concentration and it did not fall off within the periods (1-2 weeks) during which the batches of prepared diets were used. PathologyAutopsies were carried out shortly after death and the tissues fixed in either Helly's solution or formol saline. Several pieces of the liver were removed and in * Present address: Section of Environmental Carcinogenesis, Istituto Nazionale Tumori,

show abstract

The polarographic determination of dimethylnitrosamine in animal tissue

Cited by 61 publications

References 0 publications

N-Nitrosamine formation by cultures of several microorganisms

N-Nitrosamine formation by cultures of several microorganisms

Electrochemical Reduction of Prilocaine as itsN-Nitrosamine Derivative at the Mercury Electrode

Hepatic pathology in rats on low dietary levels of dimethylnitrosamine.

Contact Info

Product

Resources

About