The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin network in multiciliated cells

Yasunaga, Takayuki; Hoff, Sylvia; Schell, Christoph; Helmstädter, Martin; Kretz, Oliver; Kuechlin, Sebastian; Yakulov, Toma A.; Engel, Christina; Müller, Bárbara; Bensch, Robert; Ronneberger, Olaf; Huber, Tobias B.; Lienkamp, Soeren S.; Walz, Gerd

doi:10.1083/jcb.201502043

Cited by 49 publications

(59 citation statements)

References 44 publications

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“…A second set of actin bridges at a right angle with respect to the first set connects BBs laterally, likely also via the apical and subapical ciliary adhesions (Antoniades et al 2014). In conditions that specifically disrupt the subapical actin pool but leave the apical network unaffected, such as low doses of the actin polymerization inhibitor cytochalasin D or partial inhibition of actin regulators, BBs dock but show defects in spacing (Werner et al 2011;Ioannou et al 2013;Antoniades et al 2014;Epting et al 2015;Yasunaga et al 2015). These results support that subapical actin bridges are required for spacing BBs both along the axis of cilia orientation and perpendicular to this axis.…”

Section: Ciliary Beat Orientation Bb Organizationmentioning

confidence: 99%

“…A dense actin meshwork is present at the apical cortex in MCCs and myosin is also enriched in this area (Reverdin et al 1975;Reed et al 1984;Klotz et al 1986;Lemullois et al 1988;Sandoz et al 1988;Park et al 2006Park et al , 2008Pan et al 2007;Werner et al 2011). The formation of the apical actin network is temporally linked to BB docking and involves conserved actin regulators, such as the RhoA and Rac1GTPases, the membrane-cytoskeletal linker ezrin, the cross-linking protein filamin A, the nucleator dishevelled-associated activator of morphogenesis 1 (DAAM1), or the F-actin capping protein CapZ-interacting protein (CapZIP) (Gomperts et al 2004;Pan et al 2007;Park et al 2008;Chevalier et al 2015;Epting et al 2015;Miyatake et al 2015;Yasunaga et al 2015). The expression of several of these actin regulators is triggered by the regulatory network that controls MCC differentiation (Fig.…”

Section: Role Of the Actin Cytoskeleton In Bb Migration And Docking Tmentioning

confidence: 99%

“…Cite this article as Cold Spring Harb Perspect Biol 2016;8:a028233 ciliary transition zone that also binds to the striated rootlet in MCCs (Park et al 2008;Yasunaga et al 2015). In addition, inturned recruits the PCP effector DAAM1, and Dvl facilitates the activation of CapZIP by the kinase extracellular signal-regulated kinase 7 (ERK7) (Miyatake et al 2015;Yasunaga et al 2015).…”

Section: Multiciliated Cells In Animalsmentioning

confidence: 99%

“…In addition, inturned recruits the PCP effector DAAM1, and Dvl facilitates the activation of CapZIP by the kinase extracellular signal-regulated kinase 7 (ERK7) (Miyatake et al 2015;Yasunaga et al 2015). Another potential PCP effector affecting the actin cytoskeleton is the recently characterized flattop (FLTP) protein, which is required for BB docking in mouse MCCs (Gegg et al 2014).…”

Section: Multiciliated Cells In Animalsmentioning

confidence: 99%

“…In Xenopus MCCs, the subapical pool of actin is essential to BB proper spacing and orientation during MCC differentiation (Werner et al 2011;Ioannou et al 2013;Antoniades et al 2014;Epting et al 2015;Yasunaga et al 2015). The subapical pool consists of actin bridges connecting the BBs along the axis of ciliary beating so that each BB is connected to the tip of the ciliary rootlet of the neighboring BB facing the direction of beating (Fig.…”

Section: Ciliary Beat Orientation Bb Organizationmentioning

confidence: 99%

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Multiciliated Cells in Animals

Meunier¹,

Azimzadeh²

2016

Cold Spring Harb Perspect Biol

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Many animal cells assemble single cilia involved in motile and/or sensory functions. In contrast, multiciliated cells (MCCs) assemble up to 300 motile cilia that beat in a coordinate fashion to generate a directional fluid flow. In the human airways, the brain, and the oviduct, MCCs allow mucus clearance, cerebrospinal fluid circulation, and egg transportation, respectively. Impairment of MCC function leads to chronic respiratory infections and increased risks of hydrocephalus and female infertility. MCC differentiation during development or repair involves the activation of a regulatory cascade triggered by the inhibition of Notch activity in MCC progenitors. The downstream events include the simultaneous assembly of a large number of basal bodies (BBs)-from which cilia are nucleated-in the cytoplasm of the differentiating MCCs, their migration and docking at the plasma membrane associated to an important remodeling of the actin cytoskeleton, and the assembly and polarization of motile cilia. The direction of ciliary beating is coordinated both within cells and at the tissue level by a combination of planar polarity cues affecting BB position and hydrodynamic forces that are both generated and sensed by the cilia. Herein, we review the mechanisms controlling the specification and differentiation of MCCs and BB assembly and organization at the apical surface, as well as ciliary assembly and coordination in MCCs.

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Section: Ciliary Beat Orientation Bb Organizationmentioning

confidence: 99%

Section: Role Of the Actin Cytoskeleton In Bb Migration And Docking Tmentioning

confidence: 99%

Section: Multiciliated Cells In Animalsmentioning

confidence: 99%

Section: Multiciliated Cells In Animalsmentioning

confidence: 99%

Section: Ciliary Beat Orientation Bb Organizationmentioning

confidence: 99%

See 3 more Smart Citations

Multiciliated Cells in Animals

Meunier¹,

Azimzadeh²

2016

Cold Spring Harb Perspect Biol

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Aquatic models of human ciliary diseases

Corkins

Krneta‐Stankic

Kloc

et al. 2021

Genesis

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Cilia are microtubule‐based structures that either transmit information into the cell or move fluid outside of the cell. There are many human diseases that arise from malfunctioning cilia. Although mammalian models provide vital insights into the underlying pathology of these diseases, aquatic organisms such as Xenopus and zebrafish provide valuable tools to help screen and dissect out the underlying causes of these diseases. In this review we focus on recent studies that identify or describe different types of human ciliopathies and outline how aquatic organisms have aided our understanding of these diseases.

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Involvement of testicular DAAM1 expression in zinc protection against cadmium‐induced male rat reproductive toxicity

Chemek

Venditti

Boughamoura

et al. 2017

Journal Cellular Physiology

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In order to verify the effects of exposure to Cd and Zn on testicular DAAM1 gene and protein expression and also to ascertain their involvement in the protective role of Zn in prevent the testicular toxicity Cd-induced in male offspring rats at adult age after gestational and lactational exposure, male offspring rats, from mothers receiving either tap water, Cd, Zn, or Cd + Zn during gestation and lactation periods, were scarified on postnatal days (PND) 70. The reproductive organ (testis, epididymis, and vesicle seminal) were collected, weighed, and analyzed. The results showed that exposure to Cd in utero and through lactation decreased the relative reproductive organ weight, altered the testicular histology at the interstitial and tubular levels, and causing a significant reduction in the daily sperm production (DSP) per testis and per gram of testis, and other then altering the epididymal sperm quality. Furthermore, both mRNA and protein expression of rat testicular DAAM1 were also inhibited in Cd-treated group. Zn supply has completely corrected the most of these toxic effects. Our results imply that Zn could prevent Cd-induced testicular toxicity and sperm quality alteration in adult male rat after gestational and lactational exposure, probably via the restoration of the testicular DAAM1 expression inhibited by Cd.

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The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin network in multiciliated cells

Abstract: Inturned-mediated complex formation of NPHP4 and DAAM1 is important for ciliogenesis and ciliary function in multiciliated cells, presumably because of its requirement for the local rearrangement of actin cytoskeleton.

Cited by 49 publications

References 44 publications

Multiciliated Cells in Animals

Multiciliated Cells in Animals

Aquatic models of human ciliary diseases

Involvement of testicular DAAM1 expression in zinc protection against cadmium‐induced male rat reproductive toxicity

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