The polarity protein Angiomotin p130 controls dendritic spine maturation

Wigerius, Michael; Quinn, Dylan P.; Diab, Antonios; Clattenburg, Leanne; Kolar, Annette; Qi, Jiansong; Krueger, Stefan; Fawcett, James P.

doi:10.1083/jcb.201705184

Cited by 16 publications

(21 citation statements)

References 63 publications

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“…The function of Amot protein in neurons is poorly understood. A recent study reported that Amot is localized to dendritic spines in cultured hippocampal neurons, where it controls integrity of the postsynaptic density by regulating multi-PDZ domain protein 1 (MUPP1) and postsynaptic density-95 (PSD-95) [33]. Schanzenbächer and colleagues [34] reported that Amot is associated with neuronal homeostatic scaling and autism spectrum disorder.…”

Section: Introductionmentioning

confidence: 99%

Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice

et al. 2019

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The angiomotin (Amot)–Yes-associated protein 1 (Yap1) complex plays a major role in regulating the inhibition of cell contact, cellular polarity, and cell growth in many cell types. However, the function of Amot and the Hippo pathway transcription coactivator Yap1 in the central nervous system remains unclear. We found that Amot is a critical mediator of dendritic morphogenesis in cultured hippocampal cells and Purkinje cells in the brain. Amot function in developing neurons depends on interactions with Yap1, which is also indispensable for dendrite growth and arborization in vitro. The conditional deletion of Amot and Yap1 in neurons led to a decrease in the complexity of Purkinje cell dendritic trees, abnormal cerebellar morphology, and impairments in motor coordination. Our results indicate that the function of Amot and Yap1 in dendrite growth does not rely on interactions with TEA domain (TEAD) transcription factors or the expression of Hippo pathway–dependent genes. Instead, Amot and Yap1 regulate dendrite development by affecting the phosphorylation of S6 kinase and its target S6 ribosomal protein.

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Section: Introductionmentioning

confidence: 99%

Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice

et al. 2019

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“…It has been previously reported that Amotl2, one of the members of the Motin family of proteins, localizes to the postsynaptic machinery of the neuromuscular junction and regulates its organization (Proszynski and Sanes, 2013). Recently, Wigerius and colleagues (Wigerius et al, 2018) have reported that Amot localizes to the synapses in mature cultured neurons and regulates the postsynaptic specialization through the interaction with MUPP1 and PSD-95. In the present study we show that Amot distribution changes during neuronal development and at the early stages it localizes to dendrites and axons.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that the interaction with actin is dispensable for Amot functions in dendritic development, but it could be critical for other Amot-mediated processes. For instance, Wigerius, et al (Wigerius et al, 2018) have recently shown that in mature neurons Amot associates with actin and stabilizes actin cytoskeleton in dendritic spines, thus controlling the postsynaptic integrity and spines morphology. Therefore, depending on the developmental stage of neurons Amot could associate with different proteins and hence control different molecular processes.…”

Section: Discussionmentioning

confidence: 99%

“…Amot has been previously shown to be ubiquitously expressed in various rat brain structures and to be localized to synapses in cultured mature neurons (Wigerius et al, 2018).…”

Section: Amot Expression and Localization In Neuronsmentioning

confidence: 99%

“…The function of Amot protein in neurons is poorly understood. Recent studies demonstrated that Amot is localized to dendritic spines in cultured hippocampal neurons where it controls the integrity of postsynaptic density by regulating MUPP1 and PSD-95 (Wigerius et al, 2018). Schanzenbächer and colleagues (Schanzenbacher et al, 2016) have reported that Amot is associated with neuronal homeostatic scaling and autism spectrum disorders.…”

Section: Introductionmentioning

confidence: 99%

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Amot regulates neuronal dendritic tree through Yap1

Rojek

Krzemień

Dolezyczek

et al. 2018

Preprint

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The Amot-Yap1 complex plays a major role in the regulation of cell contact inhibition, cellular polarity and growth. However, the function of Angiomotin (Amot) and Hippo pathway transcription co-activator Yap1 in the central nervous system remains unclear. In this study, we demonstrate that Amot is a critical mediator of dendritic morphogenesis in cultured hippocampal cells and Purkinje cells in the brain. Amot function in developing hippocampal neurons depends on interactions with Yap1, which is also indispensable for dendrite growth and arborization in vitro. Conditional deletion of Amot or Yap1 in neurons leads to impaired morphogenesis of Purkinje cell dendritic trees, decreased cerebellar size, and causes defects in locomotor coordination of mutant animals. Thus, our studies identified Amot and Yap1 as novel regulators of dendritic tree morphogenesis.All rights reserved. No reuse allowed without permission.

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Angiomotin family proteins in the Hippo signaling pathway

Wang,

2024

BioEssays

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The Motin family proteins (Motins) are a class of scaffolding proteins consisting of Angiomotin (AMOT), AMOT‐like protein 1 (AMOTL1), and AMOT‐like protein 2 (AMOTL2). Motins play a pivotal role in angiogenesis, tumorigenesis, and neurogenesis by modulating multiple cellular signaling pathways. Recent findings indicate that Motins are components of the Hippo pathway, a signaling cascade involved in development and cancer. This review discusses how Motins are integrated into the Hippo signaling network, as either upstream regulators or downstream effectors, to modulate cell proliferation and migration. The repression of YAP/TAZ by Motins contributes to growth inhibition, whereas subcellular localization of Motins and their interactions with actin fibers are critical in regulating cell migration. The net effect of Motins on cell proliferation and migration may contribute to their diverse biological functions.

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The polarity protein Angiomotin p130 controls dendritic spine maturation

Abstract: Wigerius et al. identify the polarity protein AMOT-130 as vital for dendritic spine morphogenesis. They show that reduced Lats1 kinase activity in the neonatal brain is required for the recruitment of AMOT-130 to postsynaptic compartments to stabilize dendritic spines.

Cited by 16 publications

References 63 publications

Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice

Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice

Amot regulates neuronal dendritic tree through Yap1

Angiomotin family proteins in the Hippo signaling pathway

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