The plasticizer bisphenol A affects somatic and sexual development, but differently in pipid, hylid and bufonid anurans

Tamschick, Stephanie; Rozenblut-Kościsty, Beata; Ogielska, Maria; Kekenj, D; Gajewski, Franz; Krüger, Angela; Kloas, Werner; Stöck, Matthias

doi:10.1016/j.envpol.2016.05.091

Cited by 27 publications

(18 citation statements)

References 88 publications

(136 reference statements)

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“…Conclusions about amphibian sex determination have predominantly been drawn from laboratory experiments which have identified putative mechanisms and patterns of environmental sex reversal in amphibians mediated by temperature (Witschi, 1914, 1929, 1930; Hsu, Yu & Liang, 1971, Dournon et al, 1984; Lambert et al, 2018) or natural and anthropogenic chemicals (Lambert et al, 2017; Hayes et al, 2002; Pettersson & Berg, 2007; Hermelink et al, 2010; Lambert, 2015; Lambert, Skelly & Ezaz, 2016; Tamschick et al, 2016a, 2016b). By contrast, the documentation of discordances between phenotypic and genotypic sexes has not been well studied across populations of any amphibian taxon in the wild, particularly in contrasting ecological contexts.…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for sex reversal in wild populations of green frogs (Rana clamitans)

Lambert

Tran

Kilian³

et al. 2019

PeerJ

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In vertebrates, sex determination occurs along a continuum from strictly genotypic (GSD), where sex is entirely guided by genes, to strictly environmental (ESD), where rearing conditions, like temperature, determine phenotypic sex. Along this continuum are taxa which have combined genetic and environmental contributions to sex determination (GSD + EE), where some individuals experience environmental effects which cause them to sex reverse and develop their phenotypic sex opposite their genotypic sex. Amphibians are often assumed to be strictly GSD with sex reversal typically considered abnormal. Despite calls to understand the relative natural and anthropogenic causes of amphibian sex reversal, sex reversal has not been closely studied across populations of any wild amphibian, particularly in contrasting environmental conditions. Here, we use sex-linked molecular markers to discover sex reversal in wild populations of green frogs (Rana clamitans) inhabiting ponds in either undeveloped, forested landscapes or in suburban neighborhoods. Our work here begins to suggest that sex reversal may be common within and across green frog populations, occurring in 12 of 16 populations and with frequencies of 2–16% of individuals sampled within populations. Additionally, our results also suggest that intersex phenotypic males and sex reversal are not correlated with each other and are also not correlated with suburban land use. While sex reversal and intersex are often considered aberrant responses to human activities and associated pollution, we found no such associations here. Our data perhaps begin to suggest that, relative to what is often suggested, sex reversal may be a relatively natural process in amphibians. Future research should focus on assessing interactions between genes and the environment to understand the molecular and exogenous basis of sex determination in green frogs and in other amphibians.

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Section: Introductionmentioning

confidence: 99%

Molecular evidence for sex reversal in wild populations of green frogs (Rana clamitans)

Lambert

Tran

Kilian³

et al. 2019

PeerJ

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“…atrazine, estrogen, plasticizer bisphenol A, etc.) on amphibian development, both in situ or under laboratory conditions (Hayes et al 2010 ; Tamschick et al 2016 ; Lambert et al 2015 ), they remain one of the least studied animals in the context of MP contamination (de Sá et al 2018 ). It is thus important to assess whether MPs constitute an important threat to wild amphibians.…”

Section: Introductionmentioning

confidence: 99%

Microplastic ingestion by tadpoles of pond-breeding amphibians—first results from Central Europe (SW Poland)

Kolenda

Kuśmierek

Pstrowska

2020

Environ Sci Pollut Res

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Microplastics (MPs) are one of the major threats to aquatic ecosystems. Surprisingly, our knowledge of its occurrence and its impact on the organisms that dwell in small water bodies is still scarce. The aim of this study was to investigate the occurrence and chemical composition of MPs in tadpoles of pond-breeding amphibians. In total, 201 tadpoles belonging to 5 species were collected from 8 ponds located in southwestern Poland. MPs were found in all examined sites and in all studied species. Among those tested, 53 (26%) tadpoles ingested a total of 71 MPs. IR-ATR analysis revealed that particles were of anthropogenic origin and included nylon, polyurethane, polyisoprene and 1,2 polybutadiene.

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“…However, the majority of studies have been conducted with only a handful of species, with most on the so‐called amphibian model, Xenopus laevis . Given that amphibian species can be highly variable in responses to contaminants , including EDCs , it is increasingly recognized that there is a need to diversify taxonomic representation . This need has also been highlighted by multiple scientific advisory panels convened as part of the atrazine reregistration process in the United States .…”

Section: Introductionmentioning

confidence: 99%

Atrazine feminizes sex ratio in Blanchard's cricket frogs (Acris blanchardi) at concentrations as low as 0.1 μg/L

Hoskins

Boone

2017

Enviro Toxic and Chemistry

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We exposed Blanchard's cricket frogs (Acris blanchardi) to ecologically relevant concentrations (0, 0.1, 1, and 10 μg/L) of a commercial formulation of atrazine throughout the larval period to determine effects on survival, somatic growth and development (time to metamorphosis and mass at metamorphosis), and gonadal development (sex ratio at metamorphosis and the prevalence of testicular ova in phenotypic males). We tested the following hypotheses: 1) atrazine feminizes the sex ratio, 2) atrazine increases the proportion of phenotypic males with testicular ova, and 3) atrazine differentially affects somatic growth (mass at metamorphosis) and development (time to metamorphosis) for males and females. Although the control sex ratio was male-biased, exposure to 0.1 and 10 μg/L atrazine feminized sex ratios, because these treatments produced 51 and 55% fewer males than the control, respectively. We did not observe testicular ova. Atrazine did not impact survival or metamorphosis, and we did not detect sexually dimorphic impacts on time to metamorphosis or mass at metamorphosis. However, males metamorphosed 2.3 d later than females, regardless of treatment. Sex biases in timing of metamorphosis are underexplored in anurans, but if prevalent, could have important implications for theory surrounding the impact of environmental factors on metamorphosis. Our data suggest that cricket frog sex ratios are sensitive to environmentally relevant concentrations of atrazine and that feminization in the field is likely. Environ Toxicol Chem 2018;37:427-435. © 2017 SETAC.

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The plasticizer bisphenol A affects somatic and sexual development, but differently in pipid, hylid and bufonid anurans

Cited by 27 publications

References 88 publications

Molecular evidence for sex reversal in wild populations of green frogs (Rana clamitans)

Molecular evidence for sex reversal in wild populations of green frogs (Rana clamitans)

Microplastic ingestion by tadpoles of pond-breeding amphibians—first results from Central Europe (SW Poland)

Atrazine feminizes sex ratio in Blanchard's cricket frogs (Acris blanchardi) at concentrations as low as 0.1 μg/L

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