The plasticity and potential of leukemia cell lines to differentiate into dendritic cells

Guo, Qingwei; Zhang, Leling; Li, Fu; Jiang, Guosheng

doi:10.3892/ol.2012.821

Cited by 11 publications

(4 citation statements)

References 57 publications

(44 reference statements)

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“…The same trend is observed when 5-FU fibrils are added to the K562 cell (Figure S10). The dynamical change is somewhat slower in K562 cells, and we have obtained single Gaussian distribution after 30 min of addition, which may be due to the different cell types that are clarified from the peak maxima and fwhm of the individual control study of THP-1 cell and K-562 cell (Figures S8 and S9). Now, to prove that this result is solely due to 5-FU fibrillar structures, we have conducted four kind of control studies as follows: No notable change in lifetime distribution is observed of DiOC 18 in a different batch of cell lines without any additive (Figure S8 and S9).…”

Section: Resultsmentioning

confidence: 85%

Selective Self-Assembly of 5-Fluorouracil through Nonlinear Solvent Response Modulates Membrane Dynamics

et al. 2020

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Controllable self-assembly and understanding of the interaction between single metabolite fibrils and live-cell membranes have paramount importance in providing minimal treatment in several neurodegenerative disorders. Here, utilizing the nonlinear nature and peculiar hydrogen bonding behavior of the dimethyl sulfoxide (DMSO)−water mixture, the selective selfassembly of a single metabolite 5-fluorouracil (5-FU) is achieved. A direct correlation between water availability and selective selfassembly of 5-FU is ratified from the excited-state dynamics. The specific fibrillar structures of 5-FU exhibit a great potential to modulate live cell membrane fluidity and model membrane lipid distribution. After 5-FU fibril addition, a disorder of H-bonded water molecules arises several layers beyond the first hydration shell of the polar headgroups, which essentially modifies interfacial water structure and dynamics. Overall, our results shed light on the role of solvent to govern specific self-assembly and also lay the foundation accounting for the earlier stage of several diseases and multidrug resistance.

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Section: Resultsmentioning

confidence: 85%

Selective Self-Assembly of 5-Fluorouracil through Nonlinear Solvent Response Modulates Membrane Dynamics

et al. 2020

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“…Studies of human DCs ex vivo though have been constrained by difficulties; therefore, the majority of studies have been carried out in other systems. These have proven to be extremely powerful, generating a large body of fundamental data and describing numerous aspects of DC functions alongside a body of translational research using DCs for the cellular adoptive immunotherapy of infection and cancer [69]. One of the greatest problems of the ex vivo model is genetic variability, as evidenced by the fact that almost all cancer therapies are personalized.…”

Section: Evaluation Using Professional Antigen Presenting Cellsmentioning

confidence: 99%

Establishing Preferred Product Characterization for the Evaluation of RNA Vaccine Antigens

et al. 2019

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The preferred product characteristics (for chemistry, control, and manufacture), in addition to safety and efficacy, are quintessential requirements for any successful therapeutic. Messenger RNA vaccines constitute a relatively new alternative to traditional vaccine development platforms, and thus there is less clarity regarding the criteria needed to ensure regulatory compliance and acceptance. Generally, to identify the ideal product characteristics, a series of assays needs to be developed, qualified and ultimately validated to determine the integrity, purity, stability, and reproducibility of a vaccine target. Here, using the available literature, we provide a summary of the array of biophysical and biochemical assays currently used in the field to characterize mRNA vaccine antigen candidates. Moreover, we review various in vitro functional cell-based assays that have been employed to facilitate the early assessment of the biological activity of these molecules, including the predictive immune response triggered in the host cell. Messenger RNA vaccines can be produced rapidly and at large scale, and thus will particularly benefit from well-defined and well-characterized assays ultimately to be used for in-process, release and stability-indications, which will allow equally rapid screening of immunogenicity, efficacy, and safety without the need to conduct often lengthy and costly in vivo experiments.

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“…DCs have a strong stimulating effect on T lymphocytes, and even a small number of DCs and very low levels of antigen can also induce an immune response to T lymphocytes, thereby initiating cellular immune response [24][25]. The signals from DCs cause T cells to differentiate into different subtypes [26].…”

Section: Discussionmentioning

confidence: 99%

Langerhans cell-like dendritic cells treated with ginsenoside Rh2 regulate the differentiation of Th1 and Th2 cells in vivo

Liu

et al. 2019

Open Chemistry

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Ginsenoside Rh2 is one of the rare ginsenosides extracted from Panax ginseng C. A. Mey. The anti-allergic activity of ginsenoside Rh2 has been documented in some literature. In this work, an anti-allergic mechanism of ginsenoside Rh2 was investigated by focusing on the differentiation of T cells through Langerhans cells (LCs). Langerhans cell-like dendritic cells (LDCs) were generated in vitro and were used as substitute for LCs.In vivo the mRNA expression for IFN-γ and CXCR3 of T cells was increased after being injected with ginsenoside Rh2-treated LDCs thereby increasing the concentration of IFN-γ in the culture supernatants of CD3+/CD28+ T lymphocytes. However,in vitro, the expression of mRNA for CD40 and CD80 on ginsenoside Rh2-treated LDCs was up-regulated significantly and the endocytic activity of LDCs was down-regulated slightly. These findings indicate that T cells differentiation could be regulated by ginsenoside Rh2 through LDCs in vivo by altering the antigen presenting capacity, maturation and phagocytosis of LDCs.

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The plasticity and potential of leukemia cell lines to differentiate into dendritic cells

Cited by 11 publications

References 57 publications

Selective Self-Assembly of 5-Fluorouracil through Nonlinear Solvent Response Modulates Membrane Dynamics

Selective Self-Assembly of 5-Fluorouracil through Nonlinear Solvent Response Modulates Membrane Dynamics

Establishing Preferred Product Characterization for the Evaluation of RNA Vaccine Antigens

Langerhans cell-like dendritic cells treated with ginsenoside Rh2 regulate the differentiation of Th1 and Th2 cells in vivo

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