The plasma membrane proton pump gene family in cucumber

Wdowikowska, Anna; Kłobus, Grażyna

doi:10.1007/s11738-016-2152-4

Cited by 18 publications

(17 citation statements)

References 74 publications

(118 reference statements)

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“…In addition to the activity of root PM H + ‐ATPase, the expression of genes belonging to the root PM H + ‐ATPase genes family in cucumber (Wdowikowska & Klobus, ) was also measured. SHA‐treated plants showed increases in the expression of CsHA2, CSHA3, CsHA4, CsHA8, and CsHA9 genes compared to control plants 24 hr after the onset of SHA treatment.…”

Section: Resultsmentioning

confidence: 99%

“…A number of studies have shown the functional relationships between IAA, ethylene, ABA, and root PM H + ‐ATPase activity (Hager, ; Wdowikowska & Klobus, ). It is, therefore, possible that the SHA‐mediated increase in both root PM H + ‐ATPase activity and root ABA concentration are functionally linked to each other.…”

Section: Discussionmentioning

confidence: 99%

“…Reverse transcription‐qPCR analysis was performed using iQ SYBR Green supermix containing hot‐start iTaq DNA polymerase in an iCycler iQ (Bio‐Rad Laboratories). Primer pairs used to amplify cucumber plasma membrane H + ‐ATPase isoforms were taken from previously published work (Wdowikowska & Klobus, ) and synthesized by Condalab (Madrid, Spain). Standardization was carried out based on the expression of the two Cucumis sativus reference genes: glutamyl‐tRNA reductase ( HEMA1 ) (Acc.…”

Section: Methodsmentioning

confidence: 99%

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Root ABA and H⁺‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

et al. 2019

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Although the ability of humic (HA) and fulvic acids (FA) to improve plant growth has been demonstrated, knowledge about the mechanisms responsible for the direct effects of HA and FA on the promotion of plant growth is scarce and fragmentary. Our study investigated the causal role of both root PM H+‐ATPase activity and ABA in the SHA‐promoting action on both root and shoot growth. The involvement of these processes in the regulation of shoot cytokinin concentration and activity was also studied. Our aim was to integrate such plant responses for providing new insights to the current model on the mode of action of HA for promoting root and shoot growth. Experiments employing specific inhibitors and using Cucumis sativus L. plants show that both the root PM H+‐ATPase activity and root ABA play a crucial role in the root growth‐promoting action of SHA. With regard to the HA‐promoting effects on shoot growth, two pathways of events triggered by the interaction of SHA with plant roots are essential for the increase in root PM H+‐ATPase activity—which also mediates an increase in cytokinin concentration and action in the shoot—and the ABA‐mediated increase in hydraulic conductivity (Lpr).

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Root ABA and H⁺‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

et al. 2019

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“…Hence, they are vital for many life activities of organisms [3,16,43]. Until now, the members of PM H + -ATPases were identified in many species, such as in Arabidopsis, Oryza sativa, Lycopersicon esculentum, Zea mays, Cucumis sativus, Solanum tuberosum L, and Nicotiana plumbaginifolia [5,15,16,[26][27][28]. The recent release of a complete sunflower genome sequences and annotations provides convenience for PM H + -ATPase sub-gene family member identification and functional studies [29].…”

Section: Discussionmentioning

confidence: 99%

“…Previous genome-wide analysis studies reported that there are 10 and 11 PM H + -ATPase gene families in Oryza sativa and Arabidopsis thaliana, respectively [5]. There are also 12 in Lycopersicon esculentum [15], 4 in Zea mays [27], 10 in Cucumis sativus [28], 7 in Solanum tuberosum L [26], and 9 in Nicotiana plumbaginifolia [16]. Phylogenetic analysis further divided the PM H + -ATPase into five sub-clusters according to the predicted amino acid sequences [5,16].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Marowa

Liu

et al. 2020

Genes

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The P-type plasma membrane (PM) H+-ATPase plays a major role during the growth and development of a plant. It is also involved in plant resistance to a variety of biotic and abiotic factors, including salt stress. The PM H+-ATPase gene family has been well characterized in Arabidopsis and other crop plants such as rice, cucumber, and potato; however, the same cannot be said in sunflower (Helianthus annuus). In this study, a total of thirteen PM H+-ATPase genes were screened from the recently released sunflower genome database with a comprehensive genome-wide analysis. According to a systematic phylogenetic classification with a previously reported species, the sunflower PM H+-ATPase genes (HHAs) were divided into four sub-clusters (I, II, IV, and V). In addition, systematic bioinformatics analyses such as gene structure analysis, chromosome location analysis, subcellular localization predication, conserved motifs, and Cis-acting elements of promoter identification were also done. Semi-quantitative PCR analysis data of HHAs in different sunflower tissues revealed the specificity of gene spatiotemporal expression and sub-cluster grouping. Those belonging to sub-cluster I and II exhibited wide expression in almost all of the tissues studied while sub-cluster IV and V seldom showed expression. In addition, the expression of HHA4, HHA11, and HHA13 was shown to be induced by salt stress. The transgenic plants overexpressing HHA4 and HHA11 showed higher salinity tolerance compared with wild-type plants. Further analysis showed that the Na+ content of transgenic Arabidopsis plants decreased under salt stress, which indicates that PM H+ ATPase participates in the physiological process of Na+ efflux, resulting in salt resistance of the plants. This study is the first to identify and analyze the sunflower PM H+ ATPase gene family. It does not only lay foundation for future research but also demonstrates the role played by HHAs in salt stress tolerance.

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Plasma membrane H+-ATPase in plants

Mishra

Singh

Roy

2022

Cation Transporters in Plants

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The plasma membrane proton pump gene family in cucumber

Cited by 18 publications

References 74 publications

Root ABA and H⁺‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

Root ABA and H⁺‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Plasma membrane H+-ATPase in plants

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The plasma membrane proton pump gene family in cucumber

Cited by 18 publications

References 74 publications

Root ABA and H+‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

Root ABA and H+‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Plasma membrane H+-ATPase in plants

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Root ABA and H⁺‐ATPase are key players in the root and shoot growth‐promoting action of humic acids

Root ABA and H⁺‐ATPase are key players in the root and shoot growth‐promoting action of humic acids