The Plasma Membrane Potential and the Organization of the Actin Cytoskeleton of Epithelial Cells

Chifflet, Silvia; Hernández, Julio A.

doi:10.1155/2012/121424

Cited by 30 publications

(21 citation statements)

References 177 publications

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“…This plasma membrane depolarization was then shown to mediate senescence, as (i) its reversal by knocking down SCN9A allows to bypass OIS and (ii) forced plasma membrane depolarization induces a premature senescence. It appears that plasma membrane depolarization and SCN9A have so far never been linked to cellular senescence, although it is well established that they impact cell behavior (Chifflet & Hernandez, 2012; Dib‐Hajj, Yang Black & Waxman, 2013; Franco, Bortner & Cidlowski, 2006; Suzuki‐Karasaki, Suzuki‐Karasaki, Uchida & Ochiai, 2014; Willenborg et al., 2011). …”

Section: Discussionmentioning

confidence: 99%

The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway

et al. 2018

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SummaryOncogenic signals lead to premature senescence in normal human cells causing a proliferation arrest and the elimination of these defective cells by immune cells. Oncogene‐induced senescence (OIS) prevents aberrant cell division and tumor initiation. In order to identify new regulators of OIS, we performed a loss‐of‐function genetic screen and identified that the loss of SCN9A allowed cells to escape from OIS. The expression of this sodium channel increased in senescent cells during OIS. This upregulation was mediated by NF‐κB transcription factors, which are well‐known regulators of senescence. Importantly, the induction of SCN9A by an oncogenic signal or by p53 activation led to plasma membrane depolarization, which in turn, was able to induce premature senescence. Computational and experimental analyses revealed that SCN9A and plasma membrane depolarization mediated the repression of mitotic genes through a calcium/Rb/E2F pathway to promote senescence. Taken together, our work delineates a new pathway, which involves the NF‐κB transcription factor, SCN9A expression, plasma membrane depolarization, increased calcium, the Rb/E2F pathway and mitotic gene repression in the regulation of senescence. This work thus provides new insight into the involvement of ion channels and plasma membrane potential in the control of senescence.

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Section: Discussionmentioning

confidence: 99%

The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway

et al. 2018

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“…In this case, membrane hyperpolarization might be the crucial factor since it occurs both in wild-type plants in low-K and in akt1 mutants in sufficient K (Spalding et al, 1999). Membrane hyperpolarization has been shown to evoke changes in the actin cytoskeleton in mammals (Nin et al, 2009;Chifflet and Hernández, 2012), thus providing a potential link to cellular growth and shape. In summary, MR angle is determined by a membrane-delimited regulatory module that involves AKT1 and NRT2.1 but not CIPK23 or NRT1.1.…”

Section: Opposite Action Of Akt1 and Nrt21 Determines The Mr Anglementioning

confidence: 99%

Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals

et al. 2014

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As plant roots forage the soil for food and water, they translate a multifactorial input of environmental stimuli into a multifactorial developmental output that manifests itself as root system architecture (RSA). Our current understanding of the underlying regulatory network is limited because root responses have traditionally been studied separately for individual nutrient deficiencies. In this study, we quantified 13 RSA parameters of Arabidopsis thaliana in 32 binary combinations of N, P, K, S, and light. Analysis of variance showed that each RSA parameter was determined by a typical pattern of environmental signals and their interactions. P caused the most important single-nutrient effects, while N-effects were strongly light dependent. Effects of K and S occurred mostly through nutrient interactions in paired or multiple combinations. Several RSA parameters were selected for further analysis through mutant phenotyping, which revealed combinations of transporters, receptors, and kinases acting as signaling modules in K–N interactions. Furthermore, nutrient response profiles of individual RSA features across NPK combinations could be assigned to transcriptionally coregulated clusters of nutrient-responsive genes in the roots and to ionome patterns in the shoots. The obtained data set provides a quantitative basis for understanding how plants integrate multiple nutritional stimuli into complex developmental programs.

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“…It is not known if this occurs in SCs. However, dynamic variability of V m , in association with "field potentials" has been found within the architecture of neurones (axons and dendritic spines) and polarized epithelia (Bloodgood, Giessel, & Sabatini, 2009;Chifflet & Hernandez, 2012). Such spatial differences of V m can occur in electrically isolated patches of membrane in a given single cell.…”

Section: Figurementioning

confidence: 99%

Mesenchymal stem cell differentiation: Control by calcium‐activated potassium channels

Pchelintseva

Djamgoz

2017

Journal Cellular Physiology

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Mesenchymal stem cells (MSCs) are widely used in modern medicine for which understanding the mechanisms controlling their differentiation is fundamental. Ion channels offer novel insights to this process because of their role in modulating membrane potential and intracellular milieu. Here, we evaluate the contribution of calcium-activated potassium (K ) channels to the three main components of MSC differentiation: initiation, proliferation, and migration. First, we demonstrate the importance of the membrane potential (V ) and the apparent association of hyperpolarization with differentiation. Of K subtypes, most evidence points to activity of big-conductance channels in inducing initiation. On the other hand, intermediate-conductance currents have been shown to promote progression through the cell cycle. While there is no information on the role of K channels in migration of MSCs, work from other stem cells and cancer cells suggest that intermediate-conductance and to a lesser extent big-conductance channels drive migration. In all cases, these effects depend on species, tissue origin and lineage. Finally, we present a conceptual model that demonstrates how K activity could influence differentiation by regulating V and intracellular Ca oscillations. We conclude that K channels have significant involvement in MSC differentiation and could potentially enable novel tissue engineering approaches and therapies.

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The Plasma Membrane Potential and the Organization of the Actin Cytoskeleton of Epithelial Cells

Cited by 30 publications

References 177 publications

The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway

The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway

Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals

Mesenchymal stem cell differentiation: Control by calcium‐activated potassium channels

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