The Plasma Heparin Thrombin Time

Holger-Madsen, T

doi:10.1159/000205704

Cited by 14 publications

(5 citation statements)

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“…In accordance with earlier observations [8,11], the sensitivity to heparin depended strongly upon the thrombin and AT-III concentrations, whereas the fibrinogen level seemed less decisive. In addition, qualitative changes were important.…”

Section: Discussionsupporting

confidence: 92%

“…When assayed by various clotting methods, the clotting times recorded at a given heparin concentration (i.e., anticoagulant effect of heparin in the clotting systems used) are fairly similar in blood or plasma from healthy individuals [11,12,22], but may vary considerably in blood or plasma from patients, especially in 'acute phase' situations [11,12,22]. These clotting time variations are often said to reflect the so-called heparin tolerance which again may be considered as the net effect of factors influencing the anti coagulant effect of heparin in vitro, and most likely also in vivo [10].…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies on heparin tolerance, whole plasma was used as substrate [9,[11][12][13]. As a consequence, the effect of separate factors could not be satisfactorily evaluated.…”

Section: Introductionmentioning

confidence: 99%

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The Anticoagulant Effect of Heparin + Antithrombin III Using Purified Fibrinogen as Substrate

Andersen¹,

Godal²

1977

Pathophysiol Haemos Thromb

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The anticoagulant effect of heparin in test systems composed of purified factors [antithrombin III (AT-III), fibrinogen and thrombin] was studied. As expected, the sensitivity to heparin depended on the concentrations of AT-III and thrombin, whereas the fibrinogen level was less decisive. In addition, qualitative differences proved important. Thus, the sensitivity to heparin was greater with crude bovine thrombin than with highly purified thrombin from the same species. Further, the sensitivity to heparin increased following removal of cold-insoluble material from the fibrinogen preparation. Finally, during storage of purified AT-III at + 4 °C for more than 4 weeks, the sensitivity to heparin decreased more rapidly than expected from the amidolytic AT-III assays. Smaller amounts of heparm were required to give a prolonged thrombin clotting time than in whole plasma, indicating that components of normal platelet-poor plasma (besides AT-III and fibrinogen) interfere with the anticoagulant effect of heparin. The present test systems may prove suitable for the detection and evaluation of factors of importance for the so-called heparin tolerance.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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The Anticoagulant Effect of Heparin + Antithrombin III Using Purified Fibrinogen as Substrate

Andersen¹,

Godal²

1977

Pathophysiol Haemos Thromb

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“…The heparin thrombin time was determined as described elsewhere (6,8). Principle: 0.2 ml platelet-poor citratcd plasma is incubated for 3 min.…”

Section: Methodsmentioning

confidence: 99%

Heparin Resistance (Measured by the Heparin Thrombin Time) and Plasma Fibrinogen in Various Diseases

Holger-Madsen

Schioler

1962

Acta Haematol

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“…H olger-M adsen (5) using his modification (the plasma-heparin-thrombin-time) has demonstrated an increased heparin resistance in thrombotic disorders.…”

mentioning

confidence: 99%

Studies on the Heparin-Tolerance-Test

Gormsen¹

1960

Acta Haematol

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The Plasma Heparin Thrombin Time

Cited by 14 publications

References 0 publications

The Anticoagulant Effect of Heparin + Antithrombin III Using Purified Fibrinogen as Substrate

The Anticoagulant Effect of Heparin + Antithrombin III Using Purified Fibrinogen as Substrate

Heparin Resistance (Measured by the Heparin Thrombin Time) and Plasma Fibrinogen in Various Diseases

Studies on the Heparin-Tolerance-Test

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