The plant‐specific DDR factor SOG1 increases chromatin mobility in response to DNA damage

Abstract

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Long non-coding RNAs contribute to DNA damage resistance inArabidopsis thaliana

“…Recently, a report on the lncRNA COOLAIR involved in the regulation of flowering time revealed that environmental conditions resulted in alternative processing and variability in secondary structures (Yang et al, 2022). In connection with DNA repair, there is growing evidence that this involves large scale reorganization of the chromatin, e.g., by changing chromatin mobility (Meschichi et al, 2022; Meschichi and Rosa, 2023) or the formation of foci with assembly of repair factors (Hirakawa et al, 2015; Hirakawa and Matsunaga, 2019; Muñoz-Díaz and Sáez-Vásquez, 2022). It remains to be investigated whether and how lncRNAs are involved in this compartmentalization, but the candidates identified in the course of the work presented and the improved techniques to visualize RNA molecules within cells (Duncan and Rosa, 2018; Huang et al, 2020) will make these approaches possible.…”

Plant chromatin on the move: an overview of chromatin mobility during transcription and DNA repair

“…In both yeast and animals, chromatin mobility is restricted by proximity to tethering structures such as the nuclear envelope and centromeres (Chubb et al, 2002;Hajjoul et al, 2013;Heun et al, 2001;Verdaasdonk et al, 2013). In Arabidopsis, lacO/lacI lines with lacO sequences inserted in different chromosomal positions displayed different mobilities (Matzke et al, 2008(Matzke et al, , 2010Meschichi et al, 2022). Lines with insertions corresponding to nucleoplasmic regions showed similar levels of chromatin mobility, whereas a locus with subtelomeric localization showed a significant reduction in mobility compared to the other lines.…”

“…Lines with insertions corresponding to nucleoplasmic regions showed similar levels of chromatin mobility, whereas a locus with subtelomeric localization showed a significant reduction in mobility compared to the other lines. (Meschichi et al, , 2022.…”

“…Other methods that track chromatin in living cells rely on the accumulation of other fluorescence‐labeled proteins that interact with specific genomic regions or on the expression of photoactivatable fluorescent proteins linked to histones (Kruhlak et al., 2006; Wiesmeijer et al., 2008). For example, fluorescently tagged HR components have been used upon damage via radiation or genotoxic chemicals to track the mobility of DNA double‐strand breaks (DSBs) (Caridi et al., 2018; Lottersberger et al., 2015; Meschichi et al., 2022; Miné‐Hattab & Rothstein, 2012).…”

“…Changes in chromatin mobility have also been reported during cell differentiation. In Arabidopsis, the lacO /LacI and ANCHOR systems were used to measure chromatin mobility in different organs and cells with different degrees of differentiation (Matzke et al., 2019; Meschichi et al., 2021, 2022). The results obtained from studies undertaken in roots are consistent with chromatin mobility being higher in undifferentiated cells compared to fully differentiated ones (Meschichi et al., 2021).…”