The Plant Reoviridae

Francki, R. I. B.; Boccardo, G.

doi:10.1007/978-1-4899-0580-2_10

Cited by 35 publications

(15 citation statements)

References 164 publications

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“…Among the two genera of plant reoviruses, the fijiviruses have 10 segments of dsRNA and the phytoreoviruses 12 (Francki & Boccardo, 1983). In addition to having plant and insect hosts, members of the genera Fo'ivirus and Phytoreovirus have electropherotypes different from each other and from the viruses examined in this study.…”

Section: Discussionmentioning

confidence: 73%

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Winton

Lannan

Fryer

et al. 1987

Journal of General Virology

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SUMMARYThe morphological, biochemical and growth characteristics of four members of the Reoviridae, three from the fish hosts, golden shiner (Notemigonus crysoleucas), chum salmon (Oncorhynchus keta) and channel catfish (Ictalurus punetatus) and one from American oyster (Crassostrea virginica), were compared. Electron microscopy of negatively stained virions revealed icosahedral particles approximately 75 nm in diameter composed of a double capsid. Complete particles had buoyant densities in CsC1 of 1.34 to 1.36 g/ml. The viruses replicated well in several fish cell lines, forming plaque-like syncytia in monolayer cultures. Each virus could be distinguished by the range of cell lines supporting its growth. Polyacrylamide gel electrophoresis showed that the genome of each virus was composed of 11 segments of dsRNA distributed among three size classes. There were three large, three medium and five small segments in each genome and each isolate had a unique electropherotype. The segments ranged from 2.5 × 10 6 to 0"31 X 106 mol. wt. with a total genome of approximately 15 × 10 6 mol. wt. Analysis by SDS-PAGE revealed that each virus had five major structural proteins. There were two large polypeptides of approximately 135 000 and 125 000 mol. wt., one medium size polypeptide of 70000 mol. wt. and two small polypeptides of 45000 and 34000 mol. wt. Of the major structural proteins, those of approximately 70000 and 34000 tool. wt. were consistently present in the highest concentrations.Minor virion proteins were detected but were not characterized. These four viruses, isolated from aquatic animals, were unlike viruses of the six established genera of the Reoviridae.

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Section: Discussionmentioning

confidence: 73%

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Winton

Lannan

Fryer

et al. 1987

Journal of General Virology

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“…They have been classified into three different groups, Phytoreovirus, Fijivirus and the rice ragged stunt virus (RRSV) group (possible genus), based on particle morphology, number of dsRNA genome segments and vector species (Boccardo & Milne, 1984;Francki et al, 1985). Recent genomic analyses of wound tumour virus (WTV) genome segments 4 to 12 (Asamizu et al, 1985;Anzola et al, 1987Anzola et al, , 1989aXu et al, 1989;Dall et al, 1989), rice dwarf virus (RDV) genome segments 3 to 11 (Uyeda et al, 1987a(Uyeda et al, , 1989(Uyeda et al, , 1990aOmura et al, 1988Omura et al, , 1989Yamada et al, 1990;Suzuki et al, 1990a, b;Suzuki & Sugawara, 1991) and rice gall dwarf virus genome segments 8 to 10 (Koganezawa et al, 1990;Noda et al, 1991) revealed molecular relationships between the viruses within the genus Phytoreovirus.…”

Section: Introductionmentioning

confidence: 99%

Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses

Azuhata

Uyeda

Kimura

et al. 1993

Journal of General Virology

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The complete nucleotide sequences of rice blackstreaked dwarf virus (RBSDV) genome segments 8 ($8) and 7 ($7) were determined, and were found to have high sequence identities to the corresponding maize rough dwarf virus (MRDV) genome segments. RBSDV $8 and $7 consisted of 1927 and 2193 nucleotides, respectively. RBSDV $8 had a single long open reading frame (ORF)encoding 591 amino acids. RBSDV $7 had two nonoverlapping ORFs encoding 362 (ORF1) and 309 (ORF2) amino acids. The two ORFs of RBSDV $7 were inserted separately into an Escherichia coil expression vector (pKK223-3). When they were expressed in E. coli cells, the products of both ORFs migrated identically at an apparent M r of 40K. High nucleotide sequence identity was observed between RBSDV $7 and MRDV $6 (85 %), and between the terminal regions of RBSDV $8 and MRDV $7. In addition, RBSDV $7 and MRDV $6 showed 91% (ORF1 product) and 85% (ORF2 product) amino acid sequence identities.

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“…Wound tumour virus (WTV), rice dwarf virus (RDV) and rice gall dwarf virus (RGDV) are members of the Phytoreovirus genus of the family Reoviridae (Francki et al, 1985(Francki et al, , 1991 and are similar in morphology, having icosahedral double-shelled particles of approximately 65 to 70 nm in diameter (Reddy & MacLeod, 1976;Uyeda & Shikata, 1982;Omura et al, 1982). The genome of the three viruses consists of 12 dsRNA segments.…”

Section: Introductionmentioning

confidence: 99%

Viruses in the Phytoreovirus Genus of the Reoviridae Family have the Same Conserved Terminal Sequences

Kudo

Uyeda

Shikata

1991

Journal of General Virology

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The Plant Reoviridae

Cited by 35 publications

References 164 publications

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses

Viruses in the Phytoreovirus Genus of the Reoviridae Family have the Same Conserved Terminal Sequences

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