The plant LIM proteins: unlocking the hidden attractions

Abstract

Main conclusion The plant LIMs comprise two subfamilies with one (DA1/DAR) and two (2LIM) LIM domains. This review comprehensively discussed the structure and potential role of this protein family in diverse area of plant biology.The description of first eukaryote lineage-specific plant LIM domain (LIN11, ISL1, and MEC3) proteins was observed in Helianthus long back. The successive study of LIM proteins in diverse plants has shown its vital relation to development, metabolism and defence. This nascent gene family has been worked out for their role in actin dynamics, organ size determination and transcription regulation. On grounds of protein architecture, two sub-families have been delineated as DA1/DAR (one LIM domain) and 2LIMs (two LIM domains). The genomic and expression study guides to the identification of diverse subcategories. The significance of 2LIMs in regulation of actin dynamics leading to pollen growth and development has prospects to understand the plant reproductive behaviour. Interestingly, new facet of these LIMs as a transcriptional regulator in biological pathway/biosynthesis was also reported. Recently, the cumulative contribution of these features was also recognized for obtaining good quality fibre, thus giving translational outlook to this family. The DA1/DAR proteins are orchestrated with additional domains and provide a key role in regulation of organ size and tolerance to biotic and abiotic stress. This review will focus the journey of plant LIMs till date and will cover details of its structure, type, classification and functional relevance. This will provide insight to identify the potential of this gene family in the improvement of desired crop features.

The perennial fruit tree proteogenomics atlas: a spatial map of the sweet cherry proteome and transcriptome

“…Also, this list includes TFs that are specifically expressed in sweet cherry floral buds, such as LEAFY (LFY), which promotes the onset of flower formation (Goslin et al., 2017; Yamaguchi et al., 2013). In this regard, we found upregulation of transcripts in open flowers encoding LIM domain‐containing proteins that are involved in pollen development (Baltz et al., 1999; Srivastava and Verma, 2017; Weiskirchen and Günther, 2003) and Shi‐related sequence (SRS) proteins that participate in flower development (Gomariz‐Fernández et al., 2017; He et al., 2020). Another interesting example is the fact that most of the genes encoding LATERAL ORGAN BOUNDARIES (LOB) domain‐containing proteins were exclusively expressed in floral buds (Table S7).…”

Genome-Wide Identification and Expression Analysis of the DA1 Gene Family in Sweet Potato and Its Two Diploid Relatives

“…AtDA1 is one of the smaller gene families in Arabidopsis thaliana , containing only eight genes. These genes not only play a regulatory role in grain size, but also play an important role in stress in Arabidopsis thaliana [ 33 , 34 ]. DA1 encodes a ubiquitination receptor, so DA1 may interact with the ubiquitination substrate in the ubiquitination protease pathway to promote its degradation.…”

Elucidating genetic diversity and association mapping to identify SSR markers linked to 100 seed weight in chickpea (Cicer arietinum L.)

“…Emphasizing on the role of ATP binding cassette (ABC) transporter protein-encoding gene-regulating seed weight trait has been demonstrated in chickpea (Basu et al 2019) in soybean Karikariet al(2020). LIM (Lin-11, Isl-1 and Mec-3 domains) genes are recognised to be involved in actin bundles formation and contribute in a major higher-order cytoskeletal assembly in plant (Park et al 2014;Srivastava and Verma 2017). However, the role of LIM gene-regulating seed weight/size remains elusive.…”