The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

Phượng, Lê Thị; Zhao, Lei; Fitrianti, Aprilia Nur; Matsui, Hidenori; Noutoshi, Yoshiteru; Yamamoto, Mikihiro; Ichinose, Yuki; Shiraishi, Takeshi; Toyoda, Kazuhiro

doi:10.1007/s10327-019-00900-7

Cited by 10 publications

(9 citation statements)

References 31 publications

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“…The plant defense elicitor PBZ induces disease resistance in various plant species [ 17 , 20 , 24 – 26 ]. We were curious to test whether PBZ could improve powdery mildew resistance in cucumber.…”

Section: Resultsmentioning

confidence: 99%

“…PBZ improves disease resistance in crops such as maize, rice, and wheat [ 20 , 24 , 26 ]. However, to the best of our knowledge, there have been no reports on the application of PBZ in cucumber.…”

Section: Discussionmentioning

confidence: 99%

“…Powdery mildew caused by Podosphaera xanthii is a major disease in cucumber, commonly influencing leaf senescence and heavily affecting yield and nutritional quality [ 22 , 23 ]. Although the role and mode of action of PBZ in inducing disease resistance and leaf senescence have been investigated extensively in various plant species (including rice, Arabidopsis , wheat, maize, and tobacco), its efficacy has not been tested on cucurbitaceous crops [ 17 , 20 , 24 – 26 ]. Here, we report the unexpected finding that PBZ activates the ET biosynthesis and signaling pathway in cucumber, in contrast to the SA pathway in Arabidopsis [ 17 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical induction of leaf senescence and powdery mildew resistance involves ethylene-mediated chlorophyll degradation and ROS metabolism in cucumber

Zhang

et al. 2022

Horticulture Research

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Timely initiating leaf senescence is an integral part of plants’ development and importantly, an adaptive strategy for them to cope with various stresses, e.g. to limit the spread of pathogens. Powdery mildew is a major cucumber disease that promotes the initiation/progression of leaf senescence and reduces leaf photosynthesis, resulting in severe losses of yield and quality. However, how powdery mildew induces leaf senescence and how cucumber plants respond to enhance their resistance remain unclear. Here, with established agrochemical induction and pathogen inoculation systems, we demonstrate that both probenazole (PBZ) and powdery mildew activate ethylene (ET) biosynthesis and signal transduction, consequently promoting leaf senescence and enhancing plants’ resistance to powdery mildew through CsEIN3 to directly up-regulate the expression of both CsCCGs and CsRBOHs. Our analysis convincingly suggests that the regulation of leaf senescence and powdery mildew resistance is interconnected and mediated mainly by ET in cucumber.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical induction of leaf senescence and powdery mildew resistance involves ethylene-mediated chlorophyll degradation and ROS metabolism in cucumber

Zhang

et al. 2022

Horticulture Research

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“…2 ), an effective fungicide ( Uchida et al, 1973 ). Although the direct fungicidal activity of saccharin has not been reported, it is known to induce resistance to fungi in some plants ( Nakashita et al, 2002 ; Phuong et al, 2020 ; Mejri et al, 2021 ). 1,2-Benzisothiazoline-3-one ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Nematicidal Activities of Saccharin and Erythritol Against Pinewood Nematode

Kim

2022

Journal of Nematology

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The pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner & Bührer), causes pine wilt disease (PWD) resulting in severe environmental damage to pine forest ecosystems worldwide. To develop alternative strategies for managing PWD, the nematicidal activities of two sweeteners, erythritol and saccharin, were investigated. Among these two sweeteners, saccharin induced higher mortality in a dose-dependent manner. The LC50 and LC90 values of saccharin were estimated to be 0.321 M and 0.615 M, respectively. However, erythritol did not exhibit nematicidal activities. The results of our study demonstrated that saccharin is lethal to PWN and shows nematicidal effects in a dose-dependent manner. Although the mechanisms of saccharin toxicity are not yet investigated, saccharin could be used as an effective alternative for the management of PWN.

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“…BIT and presumably probenazole activate defense responses via the SA/NPR1 signaling pathway in Arabidopsis, tobacco, cucumber and rice [2,[5][6][7][8]14]. Probenazole induces Arabidopsis thaliana resistance by activating SA-signaling coupled with a consistent increase in callose deposition, which is determined by examining SA-marker genes PR1 and PR2 and suppressing JA/ET-signaling determined by examining JA-marker genes VSP2, LOX2 and PDF1.2 [15]. However, the mechanism of the interaction between rice and probenazole remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole

Wang

Zhang

et al. 2021

Metabolites

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The probenazole fungicide is used for controlling rice blast (Magnaporthe grisea) primarily by inducing disease resistance of the plant. To investigate the mechanism of induced plant defense, rice seedlings were treated with probenazole at 15 days post emergence, and non-treated plants were used for the control. The plants were infected with M. grisea 5 days after chemical treatment and incubated in a greenhouse. After 7 days, rice seedlings were sampled. The metabolome of rice seedlings was chemically extracted and analyzed using gas chromatography and mass spectrum (GC-MS). The GC-MS data were processed using analysis of variance (ANOVA), principal component analysis (PCA) and metabolic pathway elucidation. Results showed that probenazole application significantly affected the metabolic profile of rice seedlings, and the effect was proportionally leveraged with the increase of probenazole concentration. Probenazole resulted in a change of 54 metabolites. Salicylic acid, γ-aminobutyrate, shikimate and several other primary metabolites related to plant resistance were significantly up-regulated and some metabolites such as phenylalanine, valine and proline were down-regulated in probenazole-treated seedlings. These results revealed a metabolic pathway of rice seedlings induced by probenazole treatment regarding the resistance to M. grisea infection.

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The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

Cited by 10 publications

References 31 publications

Chemical induction of leaf senescence and powdery mildew resistance involves ethylene-mediated chlorophyll degradation and ROS metabolism in cucumber

Chemical induction of leaf senescence and powdery mildew resistance involves ethylene-mediated chlorophyll degradation and ROS metabolism in cucumber

Nematicidal Activities of Saccharin and Erythritol Against Pinewood Nematode

Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole

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