The Placenta in a Diabetic Pregnancy

Hiden, Ursula; Desoyé, Gernot

doi:10.1002/9781119043805.ch3

Cited by 2 publications

(3 citation statements)

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“…Diabetes-associated changes are likely to affect the placenta because enzymes, receptors, and transporters, the primary targets of circulating molecules, are expressed, often asymmetrically, on both placental surfaces [24].…”

Section: Discussionmentioning

confidence: 99%

“…It separates the maternal and foetal circulation, with which it is in contact through different surfaces. This organ can produce molecules that will affect mother and fetus independently [14,16,[22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…In diabetic gestation, maternal and foetal hyperglycaemias also influence placental metabolism, growth, and development. Diabetic insults at the beginning of gestation as in many pregestational diabetic pregnancies may have long-term effects on placental growth [21,22,24,25].…”

Section: Introductionmentioning

confidence: 99%

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Fructose 1, 6 Bisphosphate Aldolase from Gestational Diabetic Human Placenta: Purification, Identification, and Investigation of Kinetic Properties

Aksoy¹,

Doǧan²

2015

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Gestational diabetes mellitus is described as glucose intolerance at various degrees that is first detected during pregnancy. In diabetic complications, there are changes in placental function, particularly with respect to intake, transmit, and utilization of glucose, and also in glycolysis and glycolytic enzymes. The placenta possibly plays a critical role in protecting the fetus from adverse effects caused by the maternal diabetic conditions. Fructose 1,6-bisphosphate aldolase, a main glycolytic enzyme, catalyses the cleavage of fructose 1,6-bisphosphate, resulting in two three-carbon products in many cells. In this study, we principally have investigated the presence of aldolase in diabetic human placenta and then purified the enzyme. We also determined the optimum conditions of enzyme assay measurements. With this procedure, we determined the specific activity of placental aldolase as 590, 94 mU/mg protein, and aldolase was purified about 63,0 fold from gestational diabetic human placenta. The molecular weight of human placental aldolase was found as 160 kDa. In present study, substrate kinetics were also investigated, and two different K m and V max values at high and low concentrations of substrate Fructose 1,6-bisphosphate were observed. High substrate concentration range is determined as the linear substrate concentration zone. Therefore, advanced kinetic studies had been performed at this linear zone. Enzymatic assays were carried out, and substrate kinetic properties were determined. According to this determination, Vmax value of gestational diabetic placental fructose 1,6-bisphosphate aldolase was found as 939,548±60,869 U/mg and Km as 24,304±2,948 mM.

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Section: Discussionmentioning

confidence: 99%