The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity

Kohwi, Minoree; Hiebert, Laurel S.; Doe, Chris Q.

doi:10.1242/dev.061499

Cited by 32 publications

(52 citation statements)

References 30 publications

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“…Seven up is expressed in two pulses in the NB5-6T lineage Previous studies revealed that Svp is expressed in two pulses during VNC development (Kanai et al, 2005;Kohwi et al, 2011). We also noted a distinct biphasic appearance of Svp expression in the VNC (not shown).…”

Section: Research Articlesupporting

confidence: 73%

“…Recently, studies have identified additional factors involved in the downregulation of Hb: the pipsqueak-domain proteins Distal antenna and Distal antenna-related (herein referred to collectively as 'Dan') (Kohwi et al, 2011). Dan is expressed somewhat earlier than Svp, and is also maintained in a longer pulse.…”

Section: The Early Role Of Seven Up: a Temporal 'Switching Factor'mentioning

confidence: 99%

“…'switching factors'. Here, the seven up (svp) and distal antenna/distal antenna related (collectively referred to as dan herein) genes have been shown to play important roles in ensuring the switch from HbrKr, by suppressing Hb (Kanai et al, 2005;Kohwi et al, 2011;Mettler et al, 2006). Both Svp and Dan display a second wave of expression, but their function here is unknown.…”

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confidence: 99%

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Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development

et al. 2011

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Neural progenitor cells, in both vertebrates and invertebrates, go through temporal competence changes, evidenced by the generation of different classes of neurons and glia at different time points (Okano and Temple, 2009). These programmed changes are likely to be controlled by a combination of both extrinsic and intrinsic cues, and evidence points to the existence of both mechanisms in vertebrates and invertebrates. With respect to intrinsic cues, major progress has been made in the Drosophila melanogaster system, in particular in the embryonic ventral nerve cord (VNC). Here, temporal competence changes have been shown to be under control of an intrinsic temporal cascade of transcription factors, the temporal gene cascade (Brody and Odenwald, 2002;Jacob et al., 2008;Pearson and Doe, 2004). This cascade consist of the sequential expression, and function, of the Hunchback (Hb), Kruppel (Kr), Nubbin and Pdm2 (denoted collectively Pdm herein), Castor (Cas) and Grainy head (Grh) transcription factors, in a HbrKrrPdmrCasrGrh cascade. The precise progression of this cascade is an effect of mutually activating and repressing actions of the factors upon each other. In addition, studies have also identified factors that facilitate this progression, i.e. 'switching factors'. Here, the seven up (svp) and distal antenna/distal antenna related (collectively referred to as dan herein) genes have been shown to play important roles in ensuring the switch from HbrKr, by suppressing Hb (Kanai et al., 2005;Kohwi et al., 2011;Mettler et al., 2006). Both Svp and Dan display a second wave of expression, but their function here is unknown. Finally, our previous studies have also identified the existence of so-called 'sub-temporal' genes, which act downstream of the temporal genes, do not regulate temporal genes, and act to sub-divide larger temporal windows (Baumgardt et al., 2009). However, in spite of the progress in understanding temporal competence changes, it is not clear how neuroblasts switch from one competence window to the next, how window size is controlled and how windows are subdivided. Moreover, recent mathematical modelling of the temporal cascades, indicate the existence of additional players involved in the temporal competence changes observed in vivo (Nakajima et al., 2010).To address these issues, we are using the Drosophila embryonic thoracic neuroblast 5-6 (NB5-6T) as a model. This neuroblast, which can be readily identified by the specific expression of reporter genes under the control of an enhancer fragment from the ladybird early gene [lbe(K)] (De Graeve et al., 2004), is generated in each of the six thoracic VNC hemisegments. Each NB5-6T produces a mixed lineage of 20 cells, and the four last cells to be born are a set of four interneurons expressing the Apterous (Ap) LIM-homeodomain transcription factor: the Ap neurons (Baumgardt et al., 2009). The four Ap neurons can be further subdivided into three different neuronal sub-types: the Ap1/Nplp1 and Ap4/FMRFa neurons, expressing the Nplp1 and FMRFamide neurop...

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Section: Research Articlesupporting

confidence: 73%

Section: The Early Role Of Seven Up: a Temporal 'Switching Factor'mentioning

confidence: 99%

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confidence: 99%

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Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development

et al. 2011

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“…Dany's closest relatives Dan and Danr, which act partially redundantly in somatic tissues (Curtiss et al, 2007;Emerald et al, 2003;Kohwi et al, 2011Kohwi et al, , 2013, are crucial for the control of intranuclear position and silencing of the hunchback (hb) genomic locus in neuroblasts during embryogenesis (Kohwi et al, 2013). hb relocalization to the nuclear lamina, which is correlated with hb silencing and termination of Hb response competence, depends on timely Dan downregulation.…”

Section: Discussionmentioning

confidence: 99%

Drosophila danyis essential for transcriptional control and nuclear architecture in spermatocytes

et al. 2016

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The terminal differentiation of adult stem cell progeny depends on transcriptional control. A dramatic change in gene expression programs accompanies the transition from proliferating spermatogonia to postmitotic spermatocytes, which prepare for meiosis and subsequent spermiogenesis. More than a thousand spermatocyte-specific genes are transcriptionally activated in early Drosophila spermatocytes. Here we describe the identification and initial characterization of dany, a gene required in spermatocytes for the large-scale change in gene expression. Similar to tMAC and tTAFs, the known major activators of spermatocyte-specific genes, dany has a recent evolutionary origin, but it functions independently. Like dan and danr, its primordial relatives with functions in somatic tissues, dany encodes a nuclear Psq domain protein. Dany associates preferentially with euchromatic genome regions. In dany mutant spermatocytes, activation of spermatocyte-specific genes and silencing of non-spermatocyte-specific genes are severely compromised and the chromatin no longer associates intimately with the nuclear envelope. Therefore, as suggested recently for Dan/Danr, we propose that Dany is essential for the coordination of change in cell type-specific expression programs and large-scale spatial chromatin reorganization.

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“…Also, gain-offunction studies revealed that temporal TFs activate the next one in the queue and repress the next one(s) downstream, and in general terms, earlier expressed TFs in the cascade are dominant over the late ones [29,67,68]. On top of that, shifting from one temporal factor to another may also be regulated by additional factors, so-called switching TFs: Distal antenna (Dan) and Seven up (Svp) are both involved in controlling the transition from Hb to Kr within the cascade [29,69,70]. Albeit the temporal selectors play a critical role in the generation of cell type diversity, the many types of cells identified within the CNS cannot be explained exclusively by this cascade.…”

Section: Temporal Selectors Drive Cell Diversity In Developing Embryomentioning

confidence: 99%

Genetic mechanisms regulating proliferation and cell specification in the Drosophila embryonic CNS

Bahrampour¹

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Cover picture/illustration: Confocal image of the Drosophila embryos fillets at stage 12, stained for Dpn (green), Pros (red), GsbN (blue) and PH3 (white). The front cover shows the control, and the back cover is the combinatorial misexpression of da-Gal/UAS-ase, -SoxN, -wor, -hb, -Kr, -nub leads to the widespread generation of NBs and GMCs, and extensive proliferation throughout the ectoderm, contrary the underlying VNC shows normal segmentation.Published articles have been reprinted with the permission of the copyright holder.Printed in Sweden by LiU-Tryck, Linköping, Sweden, 2017

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The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity

Cited by 32 publications

References 30 publications

Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development

Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development

Drosophila danyis essential for transcriptional control and nuclear architecture in spermatocytes

Genetic mechanisms regulating proliferation and cell specification in the Drosophila embryonic CNS

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