The pig as an animal model for human pathologies: A proteomics perspective

Bassols, Anna; Costa, Cristina; Eckersall, P.D.; Osada, Jesús; Sabriá, Josefa; Tibau, J.

doi:10.1002/prca.201300099

Cited by 223 publications

(177 citation statements)

References 167 publications

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“…Pigs are known to be a species closest to humans: the vital organs in pigs and humans share high level of similarity in structure 36 . That is why they have been largely used in medical research 37 . Porous myofibrils, a basic rod-like units of a muscle, are clearly visible on cryo-SEM images.…”

Section: Resultsmentioning

confidence: 99%

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Ivashchenko

Przysiecka

Peplińska

et al. 2018

Sci Rep

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Combination therapy remains one of the most promising and intensively developed direction in cancer treatment. This study is aimed to combine and investigate the anticancer properties of silver nanoparticles (NPs) and Amanita muscaria mushroom in gel formulation. For this, hyaluronic acid was used as gel-forming agent, whereas Amanita muscaria extract was used as capping agent during silver and ultrasmall iron oxide (MAg) NPs synthesis. Amanita muscaria compounds formed NP’s surface layer and contributed anticancer properties, whereas silver NPs contributed anticancer, fluorescence and photoactive properties to the gel. Physicochemical characterization included X-ray diffraction (XRD), microscopies (SEM, cryo-SEM, TEM, confocal fluorescence), spectrofluorometric method, thermogravimetric analysis (TGA), dynamic light scattering (DLS) techniques, energy dispersive (EDS), Fourier transform infrared (FTIR) and ultraviolet–visible (UV-Vis) spectroscopies, zeta-potential and rheological measurements. Microstructure analysis of hyaluronic acid/MAg NPs gel was performed by cryo-SEM technique. We showed that hyaluronic acid is a perfect gel-forming agent from both biomedical and technological points of view. It is well-mixed with MAg NPs forming stable gel formulation; high homogeneity of hyaluronic acid/MAg NPs gel was shown by SEM EDS elemental mapping. Microstructure of the gel was found to be highly ordered and consisted of domains from perforated parallel tubular structures. This finding expanded our understanding of gels and broke the stereotype of gel structure as chaotic network of fibers. Cytotoxicity studies performed on 2D and 3D HeLa cell cultures pointed to a high potential of hyaluronic acid/MAg NPs gel for local treatment of cancer. Cell response was found to be significantly different for 2D and 3D cell cultures that was related to their different cytoarhitecture and gene expression. Thus, the results of the cellular spheroids viability showed that they were significantly more resistant to the cytotoxic action of MAg NPs and their gel formulation than 2D cell culture. Hyaluronic acid used as gelling agent in gel formulation was found to increase an effectiveness of active components (MAg NPs, Amanita muscaria extract) probably improving their transport inside HeLa spheroids.

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Section: Resultsmentioning

confidence: 99%

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Ivashchenko

Przysiecka

Peplińska

et al. 2018

Sci Rep

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“…Due to physiological, anatomical, and developmental similarities to humans, researchers have been increasingly using the pig as a model of choice for research designed to develop new treatments (1). In particular, the immune system of pigs is very similar to its human counterpart in terms of anatomy, organization, and response (2).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of apoptotic T lymphocytes and thymicepithelial cells in early postnatal pig thymus

Ceylan¹,

Alabay²

2017

Turk J Vet Anim Sci

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The thymus is a primary lymphoid organ that provides the microenvironment required for the development of T lymphocytes. Thymic epithelial cells (TECs) are among the most critical components in the thymic microenvironment supporting thymocyte selection and maturation. Apoptosis is primarily a physiologic process that plays a major role during the development of T lymphocytes. The first purpose of this study was to examine morphological changes in apoptotic thymocytes by transmission electron microscope in piglets. The second purpose was to reveal the morphological typology of TECs in piglets. Tissue samples of the thymus were taken from healthy Yorkshire race pigs of two different age groups (7 days and 1 month). Ultrastructurally, apoptotic lymphocytes showed similar morphologies, but they also had differences in distribution. In both groups a high density of apoptotic and proliferating thymocytes was found in the cortex. The rate of apoptotic thymocytes in the cortex and medulla increased with age, respectively. It was observed that the TECs had various morphological subtypes in both the cortex and the medulla. A similar cellular distribution pattern of TECs was seen in both groups. It was concluded that the morphological diversity in the TECs may be related to the form-function relationship and the metabolic process.

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“…Large animal models are useful in bridging the gap between small animal studies and clinical investigations in human subjects. The swine has been increasingly used in preclinical research as a large animal model [3, 23]. Because of the similarities in anatomy and physiology between humans and swine, the data obtained from swine can be reliably translated to humans, facilitating the development of clinical techniques and treatments.…”

Section: Introductionmentioning

confidence: 99%

A swine model of acute thrombocytopenia with prolonged bleeding time produced by busulfan

et al. 2016

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Animal models of thrombocytopenia are indispensable for evaluating the in vivo efficacy of hemostatic agents, cryopreserved platelets, and artificial platelets, but no large animal models are available. In this study, we generated a swine model of acute thrombocytopenia with prolonged bleeding times by administering the chemotherapeutic drug busulfan. First, we tested multiple doses of busulfan (4, 6, and 8 mg/kg) in pigs, and found that 6 mg/kg of busulfan is an optimal dose for producing a safe and moderate thrombocytopenia, with a platelet count of less than 30,000/µl. The pigs administered 6 mg/kg of busulfan (n=8) reached half their initial counts at day 7, counts below 30,000/µl at day 12, and their nadirs at day 15 (on average). The minimal platelet count was 14,000/µl. With this dose of busulfan (6 mg/kg), bleeding times were significantly prolonged in addition to the decrease in platelet counts (r=−0.63, P<0.01), while there were no cases of apparent hemorrhage. White blood cell counts were maintained at over 5,000/µl, and there were no infections or other adverse events including anemia or appetite or body weight loss. All pigs were sacrificed on day 16, with subsequent examination showing a significant reduction in cellularity and colony-forming units in the bone marrow, indicating that thrombocytopenia was the result of myelosuppression. In summary, administration with 6 mg/kg of busulfan induces safe and moderate thrombocytopenia with a prolonged bleeding time in swine.

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The pig as an animal model for human pathologies: A proteomics perspective

Cited by 223 publications

References 167 publications

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Ultrastructure of apoptotic T lymphocytes and thymicepithelial cells in early postnatal pig thymus

A swine model of acute thrombocytopenia with prolonged bleeding time produced by busulfan

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