The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA

Liu, Liyuan; Wang, Yingxiong; Yu, Qi

doi:10.3892/ijmm.2014.1701

Cited by 54 publications

(44 citation statements)

References 32 publications

(51 reference statements)

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“…These effects were not dependent on increased cell proliferation (Prast et al 2008). Another set of experiments, performed in mice at 5 days of pregnancy, revealed that implantation sites displayed increased PIK3CA, AKT, and RHOA expression, whereas inter-implantation site displayed increased PTEN expression; AKT was most strongly expressed in the stromal cells and luminal epithelium of the implantation site, while phosphorylated AKT (S473) was strongly expressed in stromal cells of the implantation site (Liu et al 2014). Additionally, the use of contralateral intrauterine injection of LY294002 drastically reduced the number of implanted embryos.…”

Section: Implantationmentioning

confidence: 94%

Expression, activation, and role of AKT isoforms in the uterus

Fabi¹,

Asselin²

2014

Reproduction

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The three isoforms of AKT: AKT1, AKT2, and AKT3, are crucial regulators of both normal and pathological cellular processes. Each of these isoforms exhibits a high level of homology and functional redundancy with each other. However, while being highly similar and structurally homologous, a rising amount of evidence is showing that each isoform possesses specific targets as well as preferential subcellular localization. The role of AKT has been studied extensively in reproductive processes, but isoform-specific roles are yet to be fully understood. This review will focus on the role of AKT in the uterus and its function in processes related to cell death and proliferation such as embryo implantation, decidualization, endometriosis, and endometrial cancer in an isoform-centric manner. In this review, we will cover the activation of AKT in various settings, localization of isoforms in subcellular compartments, and the effect of isoform expression on cellular processes. To fully understand the dynamic molecular processes taking place in the uterus, it is crucial that we better understand the physiological role of AKT isoforms as well as their function in the emergence of diseases.Reproduction (2014) 148 R85-R95

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Section: Implantationmentioning

confidence: 94%

Expression, activation, and role of AKT isoforms in the uterus

Fabi¹,

Asselin²

2014

Reproduction

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“…Levonorgestrel is a progestin used for emergency contraceptive and the treatment of menstrual disorders or endometriosis. Among these compounds, it is known that intrauterine injection of LY294002 [59] and sirolimus (rapamycin) [55] decreased the number of implantation sites in mice, demonstrating the validity of our CMap analysis. According to literature, there is high possibility that niclosamide, quinostatin, medrysone, gossypol and levonorgestrel are anti-implantation compounds.…”

Section: Discussionmentioning

confidence: 59%

Assessment of Embryo-Induced Transcriptomic Changes in Hamster Uterus Using RNA-Seq

Huang¹,

Zhang²,

Zhao³

et al. 2018

Cell Physiol Biochem

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Background/Aims: The mouse is widely used as an animal model for studying human embryo implantation. However, the mouse is unique in that both ovarian progesterone and estrogen are critical to implantation, whereas in the majority of species (e.g. human and hamster) implantation can occur in the presence of progesterone alone. Methods: In this study, we analyzed embryo-induced transcriptomic changes in the hamster uterus during embryo implantation by using RNA-seq. Differentially expressed genes were characterized by bioinformatic analysis. Results: We identified a total of 781 differentially expressed genes, of which 367 genes were up-regulated and 414 genes were down-regulated at the implantation site compared to the inter-implantation site. Functional clustering and gene network analysis highlighted the cell cycle process in uterus upon embryo implantation. By examining of the promoter regions of differentially expressed genes, we identified 7 causal transcription factors. Additionally, through connectivity map (CMap) analysis, multiple compounds were identified to have potential anti-implantation effects due to their ability to reverse embryo-induced transcriptomic changes. Conclusion: Our study provides a valuable resource for in-depth understanding of the mechanism underlying embryo implantation.

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“…However, 11 upstream regulators were predicted to be activated and two to be inhibited in PR cows. Among them, IGF1 27,53,54 , AGT 52,55 , TNF 56,57 , AKT and PI3K 58,59 , FGF2 20 , TGFβ1 (previously discussed) (activated in PR cows) and NFE2L2 19 (inhibited in PR cows) have been previously associated with endometrial processes and receptivity.…”

Section: Discussionmentioning

confidence: 97%

Characterization of the Endometrial Transcriptome in Early Diestrus Influencing Pregnancy Establishment in Dairy Cattle after Transfer of In-Vitro Produced Embryos

Mazzoni

Pedersen

Rabaglino

et al. 2020

Preprint

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Modifications of the endometrial transcriptome at day 7 of the estrus cycle are crucial to maintain gestation after transfer of in-vitro produced (IVP) embryos. The aim of this study was to identify genes, and their related biological mechanisms, in the endometria of recipient lactating dairy cows that become pregnant in the subsequent estrus cycle upon transfer of IVP embryos. Endometrial biopsies were taken from lactating Holstein Friesian cows on day 6-8 of the estrus cycle followed by embryo transfer in the following cycle. Animals were classified retrospectively as pregnant (PR, n=8) or nonpregnant (non-PR, n=11) cows, according to pregnancy status at 26-47 days. Extracted mRNAs from endometrial samples were sequenced with an Illumina platform to determine differentially expressed genes (DEG) between the endometrial transcriptome from PR and non-PR cows. There were 111 DEG (FDR<0.05), which were mainly related to extracellular matrix interaction, histotroph metabolic composition, prostaglandin synthesis, TGF-β signaling as well as inflammation and leukocyte activation. Comparison of these DEG with DEG identified in two public external datasets confirmed the more fertile endometrial molecular profile of PR cows. In conclusion, this study provides insights into the key early endometrial mechanisms for pregnancy establishment, after IVP embryo transfer in dairy cows.

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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA

Cited by 54 publications

References 32 publications

Expression, activation, and role of AKT isoforms in the uterus

Expression, activation, and role of AKT isoforms in the uterus

Assessment of Embryo-Induced Transcriptomic Changes in Hamster Uterus Using RNA-Seq

Characterization of the Endometrial Transcriptome in Early Diestrus Influencing Pregnancy Establishment in Dairy Cattle after Transfer of In-Vitro Produced Embryos

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