The PI3K/AKT/mTOR pathway is activated in gastric cancer with potential prognostic and predictive significance

Tapia, O.; Riquelme, Ismael; Leal, Pamela; Sandoval, Alejandra; Aedo, Susana; Weber, Helga; Letelier, Pablo; Bellolio, Enrique; Villaseca, Miguel; García, Patricia; Roa, Juan Carlos

doi:10.1007/s00428-014-1588-4

Cited by 172 publications

(152 citation statements)

References 48 publications

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…8 Activation of PI3K is also known to enhance the mucin secretion (Muc4), 9 and transcriptional upregulation of Muc4 is recognized to have antiapopitotic and mitogenic effects in various tumors. 10,11 PIK3 activation and Muc4 overexpression have also been found in gastric adenocarcinomas, 12,13 which further supports the role of ErbB2/ErbB3 – PI3K cascade activation in the formation of mucin-laden neoplastic SRCs.…”

Section: Discussionsupporting

confidence: 57%

Signet-ring cells in the skin: a case of late-onset cutaneous metastasis of gastric carcinoma and a brief review of histological approach

et al. 2017

View full text Add to dashboard Cite

Up to 10% of patients with visceral malignancies develop skin metastases during their clinical course and these metastases constitute about 2% of all skin cancers. Skin metastasis may be the first sign of a clinically silent visceral cancer or represent recurrence of an internal malignancy. In both situations, they are associated with poor prognosis, which can partly be attributed to underdiagnosis. In this paper, a case of relapsing gastric adenocarcinoma, which manifested itself as asymptomatic cutaneous papules and nodules on a patient’s head and neck, is reported and histopathological approach to the cutaneous lesions containing signet-ring cell is briefly reviewed.

show abstract

Section: Discussionsupporting

confidence: 57%

Signet-ring cells in the skin: a case of late-onset cutaneous metastasis of gastric carcinoma and a brief review of histological approach

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Since the PI3K/AKT/mTOR pathway has previously been reported to be up-regulated in gastric cancer (GC) [7, 8], we focused our study on miR-101-2, miR-125b-2 and miR-451a, which are according to TargetScan, PicTar and miRanda related to MTOR, PIK3CB and TSC1 expression, respectively. miR-101-2 is encoded by a gene located in 9p21, a chromosomal region exhibiting a high frequency of allelic loss in several cancers including liver, colon, pancreas and breast cancer, and in neuroblastoma [23–26].…”

Section: Discussionmentioning

confidence: 99%

miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in gastric cancer through regulation of the PI3K/AKT/mTOR pathway

et al. 2015

Self Cite

View full text Add to dashboard Cite

Background Gastric cancer (GC) is a deadly malignancy worldwide. In the past, it has been shown that cellular signaling pathway alterations play a crucial role in the development of GC. In particular, deregulation of the PI3K/AKT/mTOR pathway seems to affect multiple GC functions including growth, proliferation, metabolism, motility and angiogenesis. Targeting alterations in this pathway by microRNAs (miRNAs) represents a potential therapeutic strategy, especially in inhibitor-resistant tumors. The objective of this study was to evaluate the expression of 3 pre-selected miRNAs, miR-101-2, miR-125b-2 and miR-451a, in a series of primary GC tissues and matched non-GC tissues and in several GC-derived cell lines, and to subsequently evaluate the functional role of these miRNAs. Methods Twenty-five primary GC samples, 25 matched non-GC samples and 3 GC-derived cell lines, i.e., AGS, MKN28 and MKN45, were included in this study. miRNA and target gene expression levels were assessed by quantitative RT-PCR and western blotting, respectively. Subsequently, cell viability, clone formation, cell death, migration and invasion assays were performed on AGS cells. Results miR-101-2, miR-125b-2 and miR-451a were found to be down-regulated in the primary GC tissues and the GC-derived cell lines tested. MiRNA mimic transfections significantly reduced cell viability and colony formation, increased cell death and reduced cell migration and invasion in AGS cells. We also found that exogenous expression of miR-101-2, miR-125b-2 and miR-451a decreased the expression of their putative targets MTOR, PIK3CB and TSC1, respectively. Conclusions Our expression analyses and in vitro functional assays suggest that miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in primary GCs as well as in GC-derived AGS cells.

show abstract

“…The p21(now known as CDKN1A) has important roles in the proliferation of various cancer types, including glioma [18]. In addition, Wong et al reported that KDM5B promotes cell cycle progression via direct p21 repression [16].…”

Section: Kdm5b Promoted Glioma Cells Proliferation Via Downregulatingmentioning

confidence: 97%

Overexpressed KDM5B is associated with the progression of glioma and promotes glioma cell growth via downregulating p21

Dai¹,

Hu²,

Huang³

et al. 2014

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

The PI3K/AKT/mTOR pathway is activated in gastric cancer with potential prognostic and predictive significance

Cited by 172 publications

References 48 publications

Signet-ring cells in the skin: a case of late-onset cutaneous metastasis of gastric carcinoma and a brief review of histological approach

Signet-ring cells in the skin: a case of late-onset cutaneous metastasis of gastric carcinoma and a brief review of histological approach

miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in gastric cancer through regulation of the PI3K/AKT/mTOR pathway

Overexpressed KDM5B is associated with the progression of glioma and promotes glioma cell growth via downregulating p21

Contact Info

Product

Resources

About