The Phytol-rich Essential Oil from Fresh <i>Medicago hispida</i> Exerts Significant Inhibitory Activity against <i>Escherichia coli</i>

Zheng, Lixue; Ben, Leijie; Cui, Zhumei; Fu, Qian; Wang, Limei; Qi, Bin; Zhang, Yang

doi:10.3844/ajbbsp.2019.270.274

Cited by 4 publications

(10 citation statements)

References 19 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, the preliminary mechanism of EOMH against E. coli was uncovered by Scanning Electron Microscopy (SEM). This continuous work is more likely to expand and validate our previous work (Zheng et al, 2019) and to underlie the further researches on EOMH theoretically.…”

Section: Introductionsupporting

confidence: 60%

“…Nineteen constituents were identified in EOMH by GC-MS and results were presented in Table 4. These components were identical with the essential oil extracted from fresh M. hispida, but the content of each compound was different (Zheng et al, 2019). Compared with EOFMH, there were eight compounds missing in EOMH, the content of phytol in EOMH was raised to 53.6%, which was 9.84% higher than that in EOFMH.…”

Section: Gc-ms Profilementioning

confidence: 75%

“…Nevertheless, there is little information on the EOs from M. hispida. In previous work (Zheng et al, 2019), we extracted the essential oil from fresh M. hispida (EOFMH) by using stem distillation and analyzed the chemical components with gas Chromatography-Mass Spectrometry (GC-MS). At the same time, we found that EOFMH exerts significant inhibitory activity against E. coli, which is similar to that of Chloromycetin, a chemically obtained antibacterial drug and EOFMH is dominated by phytol, accounting for 48.8% of the total content of EOs in EOFMH.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Extraction Optimization of Essential Oil from Medicago hispida and Preliminary Inhibitory Mechanism Against Escherichia coli

Chu¹,

Chen²,

Wang³

et al. 2021

American Journal of Biochemistry and Biotechnology

Self Cite

View full text Add to dashboard Cite

In this study, the extraction optimization, characterization, antioxidant evaluation and preliminary inhibitory mechanism against Escherichia coli of the essential oil from dried Medicago hispida (EOMH) were explored to uncover the pending questions that had been raised in our previous work and to enlarge the industrial potential of EOMH production. The optimal parameters for EOMH extraction were: Extraction time of 7 h, particle size of 20 mesh and liquid-tosolid ratio of 30: 1 (mL/g), under which the highest yield of EOMH reached 0.31%. Nineteen constituents were identified in EOMH by gas chromatography mass spectrometry analysis, among which phytol content was up to 53.6%. These structures were further confirmed by infrared spectroscopy analysis. EOMH exerted similar scavenging capacities against Diphenyl Picryl Hydrazinyl (DPPH) and hydroxyl radicals with those of phytol. However, EOMH elicited stronger inhibitory effect on E. coli than that of phytol via shrinking E. coli cells, indicating that other factors, in addition to the antioxidant mechanism would equally affect the inhibitory effect of EOMH against E. coli, which should be deepened in the near further. In summary, present work would provide evidence for the industrial production and application of EOMH.

show abstract

Section: Introductionsupporting

confidence: 60%

Section: Gc-ms Profilementioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extraction Optimization of Essential Oil from Medicago hispida and Preliminary Inhibitory Mechanism Against Escherichia coli

Chu¹,

Chen²,

Wang³

et al. 2021

American Journal of Biochemistry and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…As summarized in Table 4, the total amount of alcohols in EODCJF was found to be 28.59% and overwhelming majority of them can be classified as long-chain alcohols. Meanwhile, according to similarity and intermiscibility principle, the abundant alkanes (Table 4) may ameliorate the dispersity of these liposoluble alcohols in EODCJF as benign solvents, thereby enhancing the interactions with test bacteria (Zheng et al, 2019). Whether or not EODCJF exerts inhibitory effects on other bacteria, such as fungus deserves further investigation.…”

Section: Discussionmentioning

confidence: 99%

Essential Oils from The Dropped Flowers of Camellia japonica: Extraction Optimization, Chemical Profile and Antibacterial Property

Kong¹,

Wang²,

Wang³

et al. 2021

American Journal of Biochemistry and Biotechnology

Self Cite

View full text Add to dashboard Cite

Camellia japonica is one of the most economically valuable species that can be taxonomically placed in the Theaceae family. Many horticultural varieties of C. japonica have been cultivated as ornamental trees worldwide, owing to their charming flowers. However, young C. japonica flowers usually drop in a "decapitated" form during the peak flowering stage, causing a resource waste. In this study, Essential Oils from the Dropped C. japonica Flowers (EODCJF) were extracted with hydrodistillation and process was optimized by response surface methodology. The chemical profile and antibacterial activity were tested by gas chromatography-mass spectrometry and by disc diffusion, respectively. The optimal extraction parameters were: Immersion time of 9 h, extraction time of 8 h and liquid-to-solid ratio of 39: 1 (mL/g), under these conditions, the yield was 3.20%. The actual yield was in compliance with the predicted result with an error margin of 3.61%. Thirty-three volatile compounds accounting for 94.46% were authenticated in EODCJF with alkanes of 53.96% and alcohols of 28.59%, respectively. EODCJF exerted certain antibacterial capacities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Bacillus pumilus. Their antibacterial activities could be contributed by the long-chain alcohols in EODCJF and alkanes may potentiate the dispersion of these liposoluble alcohols in EODCJF.

show abstract

“…Adding 50 microliters solution to 96-well plate and 150 µL microorganism liquid which was prepared by fresh nutrient medium containing 10 7~1 0 8 CFU/ml microbial strains were added into each hole, then it was placed in an incubator at 37℃ for 24 h, after that the absorbance was measured at 600 nm. Chloramphenicol solution (100~1 mg/ml) was used as the positive control and 50% DMSO as the negative control (Zheng et al, 2019;Toledo et al, 2020).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Chemical Constituents, Antibacterial and Coagulation Activity of the Essential Oil from the Stem of Artemisia argyle H. Lév.

Shan¹,

Zhou²

2021

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Artemisia argyle H. Lév. (A. argyle) is a plant of historical importance known as the "mother of herbs" in the Middle Ages. As the leaves of A. argyle have been studied more, but the stems has not been reported. This paper explores the chemical constituents, antibacterial and coagulation activities of the Essential Oil from the Stems of A. argyle (EOSAA) for better exploitation and utilization of resources. EOSAA was extracted by hydrodistillation and its chemical constituents were determined by GC-MS. Escherichia coli, Staphylococcus aureus and Bacillus pumilus were used as test microbial strains to evaluate the antimicrobial potential of EOSAA. Three indicators of Activated Partial Thromboplastin Time (APTT), Prothrombin Time (PT) and Thrombin Time (TT) were used to evaluate the coagulation activity. The extraction rate was 0.20% and 36 compounds were identified, accounting for 93.75% of the total content. Among the identified compounds, 41.72% belong to oxiterpenes, 21.31% belong to sesquiterpenes and terpenoids are extremely abundant. The main constituents were as follows: 3-Biphenylmethanol (16.01%), 2,6-Dimethyl-8-(tetrahydropyran-2yloxy)-octa-2,6-dien-1-ol (9.22%), Germacrene D (7.22%), α-Bisabolol (5.85%), L(-)-Borneol (5.75%), Eucalyptol (5.71%) and so on. EOSAA exhibited certain inhibitory effects against Escherichia coli, Staphylococcus aureus and Bacillus pumilus, the minimum inhibitory concentrations were 40, 42 and 48 mg/mL, respectively. Compared with Yunnan Baiyao, APTT, PT and TT time of high concentration (60 mg/mL) EOSAA were shortened by 28.83, 42.86 and 68.99%, respectively. The experimental results show that EOSAA is a natural coagulant with antibacterial effect and has a broad application prospect.

show abstract

The Phytol-rich Essential Oil from Fresh Medicago hispida Exerts Significant Inhibitory Activity against Escherichia coli

Cited by 4 publications

References 19 publications

Extraction Optimization of Essential Oil from Medicago hispida and Preliminary Inhibitory Mechanism Against Escherichia coli

Extraction Optimization of Essential Oil from Medicago hispida and Preliminary Inhibitory Mechanism Against Escherichia coli

Essential Oils from The Dropped Flowers of Camellia japonica: Extraction Optimization, Chemical Profile and Antibacterial Property

Chemical Constituents, Antibacterial and Coagulation Activity of the Essential Oil from the Stem of Artemisia argyle H. Lév.

Contact Info

Product

Resources

About