Tomato (Lycopersicon esculentum Mill. var Castlemart) fruit ripening was inhibited by tissue concentrations of ethanol that were produced by either exposure to exogenous ethanol vapors or synthesis under anaerobic atmospheres. Ethanol was not detected in aerobically ripened tomato fruit. Ripening was not inhibited by exposure to methanol at an equivalent molar concentration to inhibitory concentrations of ethanol, while ripening was slightly more inhibited by n-propanol than by equivalent molar concentrations of ethanol. The mottled appearance of a few ripened ethanol-treated fruit was not observed in n-propanol-treated fruit.and, at some stages of maturity, stimulated respiration and ethylene synthesis ofJapanese persimmon (10, 1 1). Acetaldehyde promoted ripening and ethylene synthesis in pears (14) and promoted color formation and a climacteric-like respiratory rise in blueberry and strawberry fruit (15). Treatment of blueberries, tomatoes, and pears with ethanol or acetaldehyde vapors increased their sugar content and sugar-acid ratio and induced desirable flavor changes (23). These compounds were less effective in promoting color changes than ethylene but had a more positive effect on flavor.A previous communication from this laboratory (26) reported inhibition of ripening of tomato fruit by ethanol. In the study described here, we have further investigated this phenomenon to better characterize the role of ethanol in fruit ripening.