The Physiological Role of the Lymphoid System. V. The Binding of Autologous (Erythrophilic) γ-Globulin to Human Red Blood Cells<sup>*</sup>

Fidalgo, B. V.; Katayama, Yoshiho; Najjar, Victor A.

doi:10.1021/bi00863a007

Cited by 34 publications

(11 citation statements)

References 7 publications

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“…Frommel et al [33] suggested that two populations of RBCbound IgG molecules were involved; one population appeared to be denatured or ag gregated IgG, and the other appeared to be proportional to the IgG concentration of the plasma. Fidalgo et al [34] also demonstrated the presence of erythrophilic (cytophilic) IgG on normal RBCs.…”

Section: Do Circulating Blood Cells Of Healthy Individuals Have Igg Amentioning

confidence: 92%

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Garratty¹

1991

Gerontology

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Normal circulating red blood cells (RBCs) and platelets have been shown to have small amounts of IgG on their membranes. The cell-bound IgG may be cytophilic (IgG nonspecifically adsorbed from the plasma) and/or IgG autoantibody. It has been suggested that most of the RBC-bound IgG is on older RBCs and is an autoantibody directed against senescent cell antigen (SCA). The accumulation of this RBC-bound IgG leads to Fc-dependent removal of senescent RBCs by macrophages in the reticuloendothelial system. RBCs also have complement components on their membrane; it is not clear how this accumulates on RBCs and whether it has a physiologic function. This small amount of nonpathogenic RBC-bound IgG is not detected by the antiglobulin test. It is still unclear whether the major difference between pathogenic and nonpathogenic IgG autoantibodies is qualitative, quantitative, or both. Seemingly healthy blood donors (1 in 1,000 donors) and patients without hemolytic anemia (up to 8% of patients) have increased amounts ( > 200 molecules/RBC) of RBC-bound IgG and complement that is detected by the antiglobulin test. This RBC-bound IgG has been shown to be an IgG autoantibody directed against blood group antigens, and/or IgG anti-idiotype, and/or IgG nonspecifically adsorbed onto the RBC membrane when plasma IgG levels are high. Most patients with autoimmune hemolytic anemia (AIHA) have RBC-bound IgG and/or complement detectable by the antiglobulin test. Most of the RBC-bound IgG is of the IgG1 subclass, whether one examines the RBCs of healthy blood donors or hospitalized patients with and without AIHA. Although the quantity of RBC-bound IgG is generally higher in patients with AIHA, there is no clear correlation with the quantity of RBC-bound IgG and the rate of in vivo RBC destruction. There is some recent evidence that the SCA autoantibody may at times be pathogenic and cause autoimmune disease.

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Section: Do Circulating Blood Cells Of Healthy Individuals Have Igg Amentioning

confidence: 92%

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Garratty¹

1991

Gerontology

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“…The functional role of naturally occurring anti-erythrocyte antibodies has been studied by opsonizing erythrocytes with purified antibodies in the absence of serum (3)(4)(5). These conditions cannot differentiate between erythrophilic (13,14) and antigen binding of added IgG. We, therefore, studied the effect of naturally occurring antibodies in the presence of serum.…”

mentioning

confidence: 99%

Naturally occurring anti-band-3 antibodies and complement together mediate phagocytosis of oxidatively stressed human erythrocytes.

Lutz

Bussolino

Flepp

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

224

159

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Treatment of erythrocytes with the thiolspecific oxidant azodicarboxylic acid bis(dimethylamide) (diamide) enhances their phagocytosis by adherent monocytes. Phagocytosis of diamide-treated erythrocytes required that the cells were opsonized with whole serum, since complement inactivation abolished phagocytosis. Opsonization with whole serum containing 20-100 times the physiological concentration of naturally occurring anti-band-3 antibodies enhanced phagocytosis of diamide-treated erythrocytes. High inputs of antiband-3 also restored phagocytosis oferythrocytes that had been incubated with complement-inactivated serum. Elevated concentrations of anti-spectrin antibodies were ineffective in whole and complement-inactivated serum.

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“…No other serum component was affected (Fig. 2) (7, [29][30][31]. T h e supernatant serum became devoid of all hemagglutinin activity since it was all bound to the erythrocytes.…”

Section: Association Of Specific Cytophilic Y-globulin With Autolmentioning

confidence: 99%

The Physiological Role of γ‐Globulin

Najjar¹

1974

Advances in Enzymology - And Related Areas of Molecular Biology

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The Physiological Role of the Lymphoid System. V. The Binding of Autologous (Erythrophilic) γ-Globulin to Human Red Blood Cells^*

Cited by 34 publications

References 7 publications

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Naturally occurring anti-band-3 antibodies and complement together mediate phagocytosis of oxidatively stressed human erythrocytes.

The Physiological Role of γ‐Globulin

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The Physiological Role of the Lymphoid System. V. The Binding of Autologous (Erythrophilic) γ-Globulin to Human Red Blood Cells*

Cited by 34 publications

References 7 publications

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Naturally occurring anti-band-3 antibodies and complement together mediate phagocytosis of oxidatively stressed human erythrocytes.

The Physiological Role of γ‐Globulin

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The Physiological Role of the Lymphoid System. V. The Binding of Autologous (Erythrophilic) γ-Globulin to Human Red Blood Cells^*