The physiological role of mitochondrial ADNT1 carrier during senescence in Arabidopsis

Fonseca‐Pereira, Paula da; Neri-Silva, Roberto; Monteiro-Batista, Rita de Cássia; Pérez-Díaz, Jorge Luis; Palmieri, Ferdinando; Araújo, Wagner L.; Fernie, Alisdair R.; Nunes‐Nesi, Adriano

doi:10.1016/j.stress.2021.100019

Cited by 1 publication

(6 citation statements)

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“…These results suggest that the deficiency of the ADNT1 gene might result in an unbalanced ATP/ADP ratio in these plants in comparison to the wild-type. These results support the previous assumption that the ADNT1 carrier plays an alternative role in stress conditions such as hypoxia or carbon starvation [5,29] which deplete cellular ATP.…”

Section: Deficiency Of Adnt1 Expression Leads To An Early "Hypoxic St...supporting

confidence: 91%

“…Because both adnt1 and line 10 mutants exhibit higher levels of AMP in roots under nonstress condition (Figure 8), it can be concluded that the downregulation of ADNT1 gene impacts the levels of adenylates in roots. It should be noted that the results, from both previous studies [4,5] and from the current one, do not point to a higher level of stress in the mutants under normal conditions. This can be assumed because (i) there were no differences in the photochemical efficiency among mutants and the wild-type at the time point 0 and (ii) the gene expression of the established senescence marker gene SAG12 did not change in the ADNT1 mutants compared with the wild-type under nonstress condition [5].…”

Section: Deficiency Of Adnt1 Expression Leads To An Early "Hypoxic St...contrasting

confidence: 73%

“…Under optimal growth conditions for Arabidopsis, the phenotype of plants with reduced expression of ADNT1 was similar to that exhibited by the wild-type, with no abnormal visible phenotypes in the mutants during the vegetative growth (Figure 1C), as previously observed [4,5]. After four weeks of cultivation, the plants were transferred to waterlogging conditions.…”

Section: Phenotypic Characterization Of Adnt1 Deficient Plants During...supporting

confidence: 70%

“…Arabidopsis thaliana seeds from wild-type (WT; ecotype Columbia-0), ADNT1 T-DNA mutant (GABI-Kat 451B06), and two previously described adnt1 antisense lines [4,5] were used in all experiments. Seeds were germinated on half-strength MS [15] (medium plates, pH 5,7, supplemented with sucrose 1% (w/v) in a growth chamber (150 µmol photons m −2 s −1 white light, 21 • C) under short-day conditions (8 h of light/16 h of dark).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…In Arabidopsis, ADNT1 expression is mainly observed in heterotrophic organs with a high demand for ATP exported from mitochondria, such as seedlings, flowers, and roots [4]. Additionally, ADNT1 expression is relatively high in senescent leaves [4,5]. However, both the physiological role and the metabolic context in which ADNT1 acts remain to be precisely elucidated.…”

Section: Introductionmentioning

confidence: 99%

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On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions

et al. 2023

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Among the adenylate carriers identified in Arabidopsis thaliana, only the AMP/ATP transporter ADNT1 shows increased expression in roots under waterlogging stress conditions. Here, we investigated the impact of a reduced expression of ADNT1 in A. thaliana plants submitted to waterlogging conditions. For this purpose, an adnt1 T-DNA mutant and two ADNT1 antisense lines were evaluated. Following waterlogging, ADNT1 deficiency resulted in a reduced maximum quantum yield of PSII electron transport (significantly for adnt1 and antisense Line 10), indicating a higher impact caused by the stress in the mutants. In addition, ADNT1 deficient lines showed higher levels of AMP in roots under nonstress condition. This result indicates that the downregulation of ADNT1 impacts the levels of adenylates. ADNT1-deficient plants exhibited a differential expression pattern of hypoxia-related genes with an increase in non-fermenting-related-kinase 1 (SnRK1) expression and upregulation of adenylate kinase (ADK) under stress and non-stress conditions. Together, these results indicated that the lower expression of ADNT1 is associated with an early “hypoxic status” due to the perturbation of the adenylate pool caused by reduced AMP import by mitochondria. This perturbation, which is sensed by SnRK1, results in a metabolic reprogramming associated with early induction of the fermentative pathway in ADNT1 deficient plants.

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Section: Deficiency Of Adnt1 Expression Leads To An Early "Hypoxic St...supporting

confidence: 91%

Section: Deficiency Of Adnt1 Expression Leads To An Early "Hypoxic St...contrasting

confidence: 73%

Section: Phenotypic Characterization Of Adnt1 Deficient Plants During...supporting

confidence: 70%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions

et al. 2023

Self Cite

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show abstract

The physiological role of mitochondrial ADNT1 carrier during senescence in Arabidopsis

Cited by 1 publication

References 58 publications

On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions

On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions

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