The physiological role of CTGF/CCN2 in zebrafish notochond development and biological analysis of the proximal promoter region

Chiou, Ming-Jyun; Chao, Tsung-Tai; Wu, Jen‐Leih; Kuo, Ching-Ming; Chen, Jyh-Yih

doi:10.1016/j.bbrc.2006.08.095

Cited by 40 publications

(48 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, little is known about the regulation of CCN in the nucleus pulposus under normal and pathological conditions. CCN2 may play a role in the development of the notochord in zebrafish, the embryonic anlagen of the intervertebral disc (33), but a similar effect on the notochord is not seen in mice lacking CCN2 (13). We have observed increasing levels of CCN2 in the degenerate nucleus pulposus and have identified TGF-␤ as an important regulator of its expression.…”

mentioning

confidence: 73%

Hypoxia-inducible Factor (HIF)-1α and CCN2 Form a Regulatory Circuit in Hypoxic Nucleus Pulposus Cells

Tran

Fujita

Huang

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:The relationship between connective tissue growth factor (CCN2) and hypoxia-inducible factor (HIF)-1 in hypoxic nucleus pulposus is unknown. Results: HIF-1␣ suppresses CCN2 expression, whereas CCN2 represses basal HIF-1␣ levels and transcriptional activity. Conclusion:In nucleus pulposus, HIF-1␣ and CCN2 form a negative regulatory circuit. Significance: Tight control of CCN2 and HIF-1 activities may play a role in disc homeostasis.

show abstract

mentioning

confidence: 73%

Hypoxia-inducible Factor (HIF)-1α and CCN2 Form a Regulatory Circuit in Hypoxic Nucleus Pulposus Cells

Tran

Fujita

Huang

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…From early disc development to maturation, many studies have reported an anabolic effect of CCN2 in maintaining the matrix components of the disc (Chiou et al, 2006;Hall-Glenn and Lyons, 2011;Huang et al, 2010;Tran et al, 2013a;Tran et al, 2013b;Tran et al, 2011). In the degenerative disc, several recent studies have demonstrated a positive correlation between CCN2 levels and the severity of degeneration (Tran et al, 2010) and the presence of pain (Peng et al, 2009).…”

Section: Different Molecular Signals and Interactions In Normal Intermentioning

confidence: 99%

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Aker¹,

Ghannam²,

Alzuabi³

et al. 2017

The Spine Scholar

View full text Add to dashboard Cite

The unique properties of the intervertebral disc (IVD) are evident in its structural complexity and functional importance for spinal support and stability. The contributions of the different cellular and extracellular components to the function of the IVD depend on their distinctive molecular features and pathways. Disruption of these molecular pathways influences the pathological changes involved in IVD degeneration. Therefore, the molecular features of the IVD have been the focus of interest for many researchers seeking to elucidate its normal functioning, potential pathologies, and appropriate therapies. The aim of the present article is to review the molecular aspects of IVD development, specific cellular markers, and the interactions between cellular and extracellular components responsible for homeostasis, degeneration and potential therapies. The literature available via PubMed and Google Scholar was reviewed and the relevant references in review articles were searched manually. Spine

show abstract

“…Cell culture, transfection, luciferase assays, and promoter-specific expression in zebrafish embryos TO2 cells from tilapia ovaries were cultured in modified Eagle's medium (MEM) and transfected as described previously (Chiou et al 2006). Briefly, the TO2 cell line consists of epithelial-like cells and was derived from a tilapia ovary (fish strain: a Tilapia mossambica 9 T. nilotica hybrid).…”

Section: Construction Of Plasmids For the Transfection Analysismentioning

confidence: 99%

“…The MO was thawed in MO buffer following a previous publication (Chiou et al 2006). The LH MO and control MO were microinjected into the one-cell stage of fertilized zebrafish eggs.…”

Section: Gene Knockdownmentioning

confidence: 99%

Molecular cloning and functional analysis of the zebrafish luteinizing hormone beta subunit (LH<beta>) promoter

Chen

Chiou

2010

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

The luteinizing hormone (LH) plays important roles in vertebrate reproduction. In the present study, we cloned and characterized the zebrafish (Danio rerio) LH subunit gene structure and promoter region. Analysis of 3.0 kb (LH3.4K~5'UTR) of the LH subunit proximal promoter region displayed maximal promoter activity in a tilapia ovary cell line (TO2 cells) after treatment with gonadotropin-releasing hormone (GnRH). Transient expression experiments with a 5'-deletion revealed at least 10 regulatory regions in the zebrafish LH subunit gene. Compared to the molecular mechanisms of other vertebrates, GnRH treatment led to the activation of zebrafish LH subunit gene transcription in ovary cells. We demonstrated that LH subunit gene transcription increased with 6 h of treatment with GnRH but was repressed by protein kinase C, mitogen-activated protein kinase, and calcium in the TO2 cell line. To study promoter-specific expression, we constructed an LH subunit (LH3.4k~5'UTR) promoter region-driven green fluorescent protein (GFP), and the results indicated that LH promoter-driven GFP transcripts appeared in the pituitary gland. For the gene knockdown study, we targeted knockdown of the LH subunit gene by two antisense morpholino oligonucleotides that resulted in serious abnormalities and death during zebrafish embryogenesis. These results suggest that the LH plays important roles in reproduction and general embryonic development in zebrafish.

show abstract

The physiological role of CTGF/CCN2 in zebrafish notochond development and biological analysis of the proximal promoter region

Cited by 40 publications

References 23 publications

Hypoxia-inducible Factor (HIF)-1α and CCN2 Form a Regulatory Circuit in Hypoxic Nucleus Pulposus Cells

Hypoxia-inducible Factor (HIF)-1α and CCN2 Form a Regulatory Circuit in Hypoxic Nucleus Pulposus Cells

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Molecular cloning and functional analysis of the zebrafish luteinizing hormone beta subunit (LH<beta>) promoter

Contact Info

Product

Resources

About