The physiological responses of terrestrial cyanobacterium<i>Nostoc flagelliforme</i>to different intensities of ultraviolet-B radiation

Shen, Shi-gang; Jia, Shiru; Yan, Rong-rong; Wu, Yi-Kai; Wang, Huiyan; Lin, Ya-Hui; Zhao, Dongxue; Tan, Zhi-Jie; Lv, Hexin; Han, Peipei

doi:10.1039/c8ra04024a

Cited by 8 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the decreased carotenoid content upon UVR sorption may be a result of scavenging ROS, as consistent with previous studies [3, 79]. However, other studies have also observed increased production of carotenoids in cyanobacteria upon UVB irradiation [4, 80], again due to their antioxidant functions. Thus, it appears that if/when there are sufficient carotenoids available to remove ROS, these compounds may be used to quench ROS and in doing so, these compounds are destroyed.…”

Section: Discussionsupporting

confidence: 90%

Phyllosilicates as protective habitats of filamentous cyanobacteria Leptolyngbya against ultraviolet radiation

Kugler

Dong

2019

PLoS ONE

View full text Add to dashboard Cite

Phototrophic cyanobacteria are limited in growth locations by their need for visible light and must also cope with intermittent ultraviolet radiation (UVR), especially in extreme environments such as deserts and on early Earth. One survival method for cyanobacteria is growing endolithically within minerals such as micas, gypsum, and quartz minerals. However, the capability of different mica minerals to protect cyanobacteria from UVR, while at the same time allowing transmission of photosynthetically active radiation (PAR), has only been minimally examined. In this study, we performed laboratory incubation experiments to demonstrate that a model filamentous cyanobacterium, Leptolyngbya sp., can colonize micas, such as muscovite, phlogopite, and biotite. After inoculation experiments confirmed that these cyanobacteria grew between the sheets of mica, Leptolyngbya sp. colonies were exposed to UVB and UVC for up to 24 hrs, and the level of survival was determined using chlorophyll a and carotenoid assays. Of the three micas investigated, muscovite, being an Fe-poor and Al-rich mica, provided the least attenuation of UVR, however it transmitted the most visible light. Fe-rich biotite provided the best UVR shielding. Phlogopite, apparently because of its intermediate amount of Fe, showed the greatest ability to shield UVR while still transmitting an adequate amount of visible light, making it the ideal habitat for the cyanobacterium. Upon exposure to UVR, significant shifts in several important fatty acids of the cyanobacterium were detected such as linolenic acid and oleic acid, 18:3ω3 and 18:1ω9c, respectively. These cellular changes are interpreted to be a consequence of UVR and other accessory stress (such as O 3 ).

show abstract

Section: Discussionsupporting

confidence: 90%

Phyllosilicates as protective habitats of filamentous cyanobacteria Leptolyngbya against ultraviolet radiation

Kugler

Dong

2019

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The soluble sugar sucrose, a disaccharide consisting of glucose and fructose, has been seen to have a role in stress tolerance in both plants and cyanobacteria [18,32] which is consistent with the opposite effect between UV-A exposure and PAR only exposure (Figure 5).…”

Section: Effects Of Uv Exposure On C Fritschiisupporting

confidence: 68%

“…This trend is also seen with mystiric acid ( Figure 6 , comparing UV-A and UV-B). Saturated fatty acids provide energy for the rebuilding of photosynthetic apparatus which could be damaged directly by UV-B but to a lesser extent during UV-A irradiation [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…[ 17 ] to name a few. Fewer studies have focused on untargeted metabolomic analyses for wider profiling of metabolites produced and affected by UV, but examples include metabolite level changes in Nostoc flagelliforme to different intensities of UV-B [ 18 ] and a proteomic and metabolomics investigation of Nostoc punctiforme ATCC 29133 during UV-A shock and short-term stress [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912

et al. 2021

View full text Add to dashboard Cite

Ultraviolet A (UV-A) is the major component of UV radiation reaching the Earth’s surface, causing indirect damage to photosynthetic organisms via the production of reactive oxygen species (ROS). In comparison, UV-B causes both direct damage to biomolecules and indirect damage. UV-B is well studied in cyanobacterial research due to their long evolutionary history and adaptation to high levels of UV, with less work on the effects of UV-A. In this study, the response of key metabolites in Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR, 15 µmol·m−2·s−1) supplemented with UV-A (11 µmol·m−2·s−1) was investigated using gas chromatography- mass spectrometry (GC-MS). Results showed an overall significant increase in metabolite levels up to 24 h of UV-A exposure. Compared with previously reported UV-B (PAR + UV-B) and PAR only results, UV-A showed more similarity compared to PAR only exposure as opposed to supplemented UV-B. The amino acids glutamate, phenylalanine and leucine showed differences in levels between UV (both supplemented UV-A and supplemented UV-B) and PAR only (non-supplemented PAR), hinting to their relevance in UV stress response. The fatty acids, palmitic and stearic acid, showed positive log2 fold-change (FC) in supplemented UV-A and PAR only experiments but negative log2 FC in UV-B, indicating the more harmful effect of UV-B on primary metabolism. Less research has been conducted on UV-A exposure and cyanobacteria, a potential environmental stimuli for the optimisation of metabolites for industrial biotechnology. This study will add to the literature and knowledge on UV-A stress response at the metabolite level in cyanobacteria, especially within the less well-known species C. fritschii.

show abstract

“…[13] and Chlorogloeopsis fritschii ( C. fritschii) , PCC 6912, [14] to name a few. Other studies conducted have sought to evaluate changes at the protein level [15,16], targeted and untargeted metabolomic analysis using different intensities of UV-B [17] and combined metabolomic and proteomic analysis during UV-A exposure [18].…”

Section: Introductionmentioning

confidence: 99%

Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure

et al. 2019

View full text Add to dashboard Cite

Cyanobacteria have many defence strategies to overcome harmful ultraviolet (UV) stress including the production of secondary metabolites. Metabolomics can be used to investigate this altered metabolism via targeted and untargeted techniques. In this study we assessed the changes in the intra- and extracellular low molecular weight metabolite levels of Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR) supplemented with UV-B (15 µmol m−2 s−1 of PAR plus 3 µmol m−2 s−1 of UV-B) and intracellular levels during 48 h of PAR only (15 µmol m−2 s−1) with sampling points at 0, 2, 6, 12, 24 and 48 h. Gas chromatography–mass spectrometry (GC–MS) was used as a metabolite profiling tool to investigate the global changes in metabolite levels. The UV-B time series experiment showed an overall significant reduction in intracellular metabolites involved with carbon and nitrogen metabolism such as the amino acids tyrosine and phenylalanine which have a role in secondary metabolite production. Significant accumulation of proline was observed with a potential role in stress mitigation as seen in other photosynthetic organisms. 12 commonly identified metabolites were measured in both UV-B exposed (PAR + UV-B) and PAR only experiments with differences in significance observed. Extracellular metabolites (PAR + UV-B) showed accumulation of sugars as seen in other cyanobacterial species as a stress response to UV-B. In conclusion, a snapshot of the metabolome of C. fritschii was measured. Little work has been undertaken on C. fritschii, a novel candidate for use in industrial biotechnology, with, to our knowledge, no previous literature on combined intra- and extracellular analysis during a UV-B treatment time-series. This study is important to build on experimental data already available for cyanobacteria and other photosynthetic organisms exposed to UV-B.

show abstract

The physiological responses of terrestrial cyanobacteriumNostoc flagelliformeto different intensities of ultraviolet-B radiation

Abstract: The findings would improve the understanding of physiological responses ofN. flagelliformeto UV-B radiation.

Cited by 8 publications

References 50 publications

Phyllosilicates as protective habitats of filamentous cyanobacteria Leptolyngbya against ultraviolet radiation

Phyllosilicates as protective habitats of filamentous cyanobacteria Leptolyngbya against ultraviolet radiation

Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912

Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure

Contact Info

Product

Resources

About