The Physiological Function and Potential Role of the Ubiquitous Na+/H+ Exchanger Isoform 8 (NHE8): An Overview Data

Abstract

The Na+/H+ exchanger transporters (NHE) play an important role in various biologic processes including Na+ absorption, intracellular pH homeostasis, cell volume regulation, proliferation, and apoptosis. The wide expression pattern and cellular localization of NHEs make these proteins pivotal players in virtually all human tissues and organs. In addition, recent studies suggest that NHEs may be one of the primeval transport protein forms in the history of life. Among the different isoforms, the most well-characterized NHEs are the Na+/H+ exchanger isoform 1 (NHE1) and Na+/H+ exchanger isoform 3 (NHE3). However, Na+/H+ exchanger isoform 8 (NHE8) has been receiving attention based on its recent discoveries in the gastrointestinal tract. In this review, we will discuss what is known about the physiological function and potential role of NHE8 in the main organ systems, including useful overviews that could inspire new studies on this multifaceted protein.

Jian-Pi-Yin decoction attenuates lactose-induced chronic diarrhea in rats by regulating GLP-1 and reducing NHE3 ubiquitination and phosphorylation

“…Apical membrane NHE3 activity in epithelial cells is regulated by a variety of mechanisms, and acute regulation focuses on NHE3 cycling between the plasma membrane and intracellular compartments, which refers to the reciprocal flow of NHE3 from the surface of the epithelial cell membrane and intracellular vesicles [ 28 ]. The phosphorylation and ubiquitination of NHE3 play an important role in the complex regulatory mechanism of endocytosis and are important factors regulating the active expression of NHE3 [ 29 ].…”

Intestine‐on‐a‐chip for intestinal disease study and pharmacological research

“… 60 Intestinal mucin can also be stained by using wheat germ agglutinin (WGA) labeled with FITC 94 . NHE3 is expressed in the apical of intestinal epithelial cells and plays a major role in electroneutral sodium absorption, 95 which can also represent the apical polarization of the epithelial monolayers. The vertical and horizontal cross‐section of NHE3 (green) detected by fluorescence confocal imaging demonstrated the intestinal apical polarization of differentiated cells (Figure 5C).…”

Na+/H+ Exchangers (NHEs) in Mammalian Sperm: Essential Contributors to Male Fertility

“…NHE1 activity is highly sensitive to amiloride, ethylisopropoylamiloride (EIPA), 5-(N,N-dimethyl)-amiloride (DMA), and 5-(N,N-hexamethylene)-amiloride (HMA), and cariporide, all known inhibitors of NHEs (for reviews of NHE inhibitor structures, specificity and applications see) [6,7,59,60,62,125,126]. For a detailed review on NHE1 structure and transport see [61].…”