The Physiological effect of Type II Diabetes Mellitus on Nitrotyrosine and some Biochemical Parameters

Abstract

Background: Diabetes mellitus is a chronic metabolic disease and characterized by elevated glucose, factors that contribute T2D are the body's resistance to insulin and falling production insulin by the beta cells (β-cells) of the pancreas. Objectives: This study is planned to assess the nitrotyrosine (3-NT), lipid profile and UA in diabetes mellitus patients according age groups (35-75), Sex and smoking. Materials and Methods: It was conducted on (42) patients T2D subjects and 25controls .They were divided into 3 groups according to age groups (35-75 years), sex (male and female) and smoking (smokers and non-smokers). Blood was withdrawn for estimation of serum Lipid profile (Total cholesterol, Triglyceride, LDL, HDL, and VLDL) test , FBS, UA and 3-nitrotyrosine (3-NT) concentrations . The parameters were determined by using spectrophotometer by liquid chemistry and Estimate 3-nitrotyrosine by Human 3-NT ELISA Kit. Results: The results showed a physiological effect diabetic (T2D) on Uric acid, lipid profile and NT levels and results were also compared with the control group and groups with each other .Where a high significant increase was observed for the four age groups but except HDL . In addition, the results showed no significant difference between the sexes (Males-Females) for Parameters .While there was a high significant increase for both sexes for the Parameters compared with the control group except HDL also. It showed a significant increase of NT and Biochemical Parameters of smoking patients compared with the blood serum of non-smoking patients. Conclusions: In this study NT was undetected in healthy subjects but found an increase in the level NT and Some Biochemical Parameters in the blood of T2D patients. 3-NT can be considered as an indicator for the detection of diabetes because it is a product of oxidative stress. These indicators increased with patients who smoke.