The physico-chemistry of adhesions of protein resistant and weak polyelectrolyte brushes to cells and tissues

Abstract

Polymer brush adhesion to cells is mediated by a combination of electrostatic and hydrogen bonding interactions, regulated by the glycocalyx.

Current Research on Polyelectrolyte Nanostructures: From Molecular Interactions to Biomedical Applications

“…T interaction of human keratinocyte cells and human primary epidermal keratinocyt monolayers with the cationic polyelectrolyte poly(dimethylaminoethyl methacryla (PDMAEMA), the anionic polyelectrolyte poly(acrylic acid) (PAA) and the neutral hydr philic polymer poly(oligoethylene glycol methacrylate) (POEGMA) were studied (Figu 7). The length and the high density of POEGMA side-chains resulted in low adhesi forces in agreement with the known resistance of POEGMA brushes to proteins and r The adhesion of polymers to epithelial cell monolayers and soft tissues has been quantified by attaching macromolecular brushes on a silica NPs on the edge of an AFM tip [13]. The experiments were supported by measurements on self-assembled monolayers with different properties such as hydrophilicity, charge and hydrogen bonding.…”

“…The adhesion of polymers to epithelial cell monolayers and soft tissues has be quantified by attaching macromolecular brushes on a silica NPs on the edge of an AF tip [13]. The experiments were supported by measurements on self-assembled monola ers with different properties such as hydrophilicity, charge and hydrogen bonding.…”

“…The difference was attributed to the differences of the glycocalyx layers between the two cell cultures. Deeper insights to the physical interactions between the brushes and the cells were obtained by enzymatic cleavage of the glycocalyx components [13].…”

“…Characteristic examples of nanostructures are the multi-scale organization of triblock polyelectrolyte hydrogels [8], the self-organized protein-polyelectrolyte complexes [9] and the hierarchical structure in thermoresponsive triblock polyelectrolytes [10]. The interactions of star-like micelles with hydrophobic counterions [11], polyelectrolytes with nanoparticles (NPs) of patchy surface charge [12] and anionic and cationic polyelectrolytes with cells [13] are intriguing new research works. Hydrogels for wound healing [14] and cartilage regeneration [15] are a couple of indicative possibilities offered by polyelectrolyte nanostructures.…”

Evaluation of the Photocatalytic Activity and Anticorrosion Performance of Electrospun Fibers Doped with Metallic Oxides

“…Finally, a surprising result was observed when the sample had been immersed in ultrapure water (24 h immersion), because a considerable improvement of the adhesion coating was clearly presented, showing the best adhesion properties without any amount of removal of the electrospun coating (rating value of 5). This high value is associated to the weak polyelectrolyte nature of the polymeric precursor of PAA, which can display a high density of carboxylic acid groups and negative charges, making possible a complex balance of interactions (mostly electrostatic and van der Waals interactions) that can also promote strong adhesion onto the substrate [60]. Finally, a total change in the appearance of the electrospun fibers was also appreciated and associated to the pH-sensitive swelling behavior of PAA after immersion in ultrapure water [61][62][63].…”