The Physical Properties of Bean Common Mosaic Virus Distributed in Ukraine

Abstract

Bean common mosaic virus (BCMV) is one of the most widespread in bean crops viruses belonging to Potyviridae family. The present study was conducted to know the physical properties of BCMV distributed in Ukraine. Methods. Visual diagnosis, biological testing of the virus, electron microscopy, polymerase chain reaction with reverse transcription (RT-PCR). Results. The presence of BCMV in the plants showing clear viral infection symptoms was established by RT-PCR. The virus was purified from BCMV-infected bean tissues by modified procedure involved clearing with 2.5 % of Triton X-100 and concentrating by centrifugation with linear 10-50 % sucrose gradient in 0.1 M Tris-thioglycolic acid. The yield of purified virus was 3-4 mg/100 g of infected leaves. The determination of physical properties using the same buffer showed that infectivity of crude was lost by heating at 60-65° C for 10 minutes, dilution end point (DEP) was 10 4 and longevity in vitro (LIV) was 1-2 days at 4º C or 3-4 days at 20-25° C storage in vitro. This virus is long flexuous filamentous particles approximately 740-750 nm in size. Conclusions. The results added physical properties to the knowledge of BCMV which helps in determining management strategies against the virus.

Changes of Several Metabolic Parameters of Soya Inoculated With Phytopathogens at Application Nanochelates

“…2). The expected sizes of amplifying DNA fragments for BCMV were 391base pair (bp) (Kyrychenko & Prylipko, 2020).…”

“…The RT was performed using Reverta kits (AmpliSens), according to the manufacturer's instruction. The work used primers that allow to identify a fragment of the nucleotide sequence encoding the protein gene of the envelope with the following nucleotide sequences: direct primer 5'-ttcggacgtcgtgagtgtta-3 'and reverse 5'cccgagtccacattaattcc-3', the size of the amplification product was 391 bp (Kyrychenko & Kovalenko, 2018;Kyrychenko et al, 2019;Kyrychenko & Prylipko, 2020). For PCR, 2 μL of the template genomic DNA was amplified in a 25-μL of total volume containing 1 × reaction buffer, 5 pmol of each primer, 0.3 mM dNTPs, 1.25 U of TaqDNA polymerase and nuclease-free water.…”

“…Some of these viruses have a wide range of host plants. Yep, Bean common mosaic virus (BCMV) causes significant loss of bean productivity but can also infecting of soybeans (Lee et al, 2015;Kyrychenko & Prylipko, 2020). In research by Zhou et al (2014) identified 30 isolates of BCMV that are capable of infecting soybeans.…”