The physical Location of Genes cdc2 and prh1 in Maize (Zea Mays L.)

Ren, Naixia; Song, Yun‐Chun; Bi, Xuezhi; Ding, Yi; Liu, L. H.

doi:10.1111/j.1601-5223.1997.00211.x

Cited by 17 publications

(9 citation statements)

References 52 publications

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“…Two sets of primers and probe were used. One set was specific for the pat gene which encodes phosphinothricin acetyltransferase (the target gene used to detect the T25 transformation event; forward, 5¢-CTGTCCAATCGTAAGCGTTCC-3¢; reverse, 5¢-GGTGTTGTGGCTGGTATTGCT-3¢; probe, 5¢-FAM TM -AGCCTTCCAGGGCCCAGCGT-TAMRA TM -3¢); and the other set was specific to the Z. mays cdc2 gene (forward, 5¢-TGTACAAGGCGCTGGACAAG-3¢; reverse, 5¢-GCTCGAGGCGGATCTTCTT-3¢; probe, 5¢-FAM TM -CCACCAACGAGACGATCGCGCT-TAMRA TM -3¢) (Ren et al 1997). The endogenous control primers served two purposes.…”

Section: Dna Extraction and Real-time Pcrmentioning

confidence: 99%

A study of crop-to-crop gene flow using farm scale sites of fodder maize (Zea mays L.) in the UK

Weekes¹,

Allnutt²,

Boffey

et al. 2006

Transgenic Res

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From 2000 to 2003 a range of Farm Scale Evaluation (FSE) trials were established in the UK to assess the effect of the release and management of herbicide tolerant (HT) crops on arable weeds and invertebrates. The FSE trials for maize were also used to investigate crop-to-crop gene flow and to develop a statistical model for the prediction of gene flow frequency that can be used to evaluate current separation distance guidelines for GM crops. Seed samples were collected from the non-GM half of 55 trial sites and 1,055 were tested for evidence of gene flow from the GM HT halves using a quantitative PCR assay specific to the HT (pat) gene. Rates of gene flow were found to decrease rapidly with increasing distance from the GM source. Gene flow was detected in 30% of the samples (40 out of 135) at 150 m from the GM source and events of GM to non-GM gene flow were detected at distances up to and including 200 m from the GM source. The quantitative data were subjected to statistical analysis and a two-step model was found to provide the best fit for the data. A dynamic whole field model predicted that a square field (150 m x 150 m in size) of grain maize would require a separation distance of 3 m for the adjacent crop to be below a 0.9% threshold (with <2% probability of exceeding the threshold). The data and models presented here are discussed in the context of necessary separation distances to achieve various possible thresholds for adventitious presence of GM in maize.

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Section: Dna Extraction and Real-time Pcrmentioning

confidence: 99%

A study of crop-to-crop gene flow using farm scale sites of fodder maize (Zea mays L.) in the UK

Weekes¹,

Allnutt²,

Boffey

et al. 2006

Transgenic Res

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show abstract

“…These results would be helpful for using wild rice species in the development of cultivated rice. Yan et al (1998) and Ren et al (1997).…”

Section: Introductionmentioning

confidence: 96%

Comparative analysis of genomes in Oryza sativa, O. officinalis and O. meyeriana with C 0 t-1 DNA and genomic DNA of cultivated rice

Lan

Wang

et al. 2007

Front. Agric. China

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Fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) were applied to somatic chromosome preparations of Oryza sativa, O. officinalis and O. meyeriana with labeled probes of C 0 t-1 DNA and genomic DNA from cultivated rice. The coverage percentage (%) and size (Mb) of C 0 t-1 DNA in O. sativa, O. officinalis and O. meyeriana were 47.1P0.16, 38.61P0.13, 44.38P0.13 and 212.33P1.21, 269.42P0.89, 532.56P1.68, respectively. The coverage percentage and size of probe signals with gen omic DNA from O. sativa in O. officinalis and O. meyeriana were 91.0%, 93.6% and 634 Mb, 1 123 Mb respectively, in which there were 365 and 591 Mb in O. officinalis and O. meyeriana which came from O. sativa genomic DNA not from repetitive sequences of O. sativa, and the uncovered genome size in O. officinalis and O. meyeriana was 64 and 78 Mb, respectively. In addition, karyotype analysis was conducted based on the signal bands of C 0 t-1 DNA in O. sativa, O. officinalis and O. meyeriana. The results showed that highly and moderately repetitive sequences in Oryza genus were conserved as the functional genes during the evolution process. The repetitive sequence reduplication might be one of the important causes of genome enlargement in O. officinalis and O. meyeriana; the O. officinalis genome enlarged more slowly compared with O. meyeriana. Based on the above results, it is concluded that O. officinalis and O. meyeriana formed by reduplication, rearrangement and gene selective loss during the evolution process.Comparative analysis of genomes in Oryza sativa, O. officinalis and O. meyeriana with C 0 t-1 DNA and genomic DNA of cultivated rice

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“…They have a common ancestor, a close relationship and a high homology in DNA level. This provides a possibility to localize the rice BAC clone on the maize chromosomes and to localize, reveal and clone the maize genes based on the corresponding known rice genes (Ren et al, 1997). The advanced BAC-FISH technology is the prerequisite to achieve this goal.…”

Section: Discussionmentioning

confidence: 99%

Technological exploration of BAC-FISH on mitotic chromosomes of maize

et al. 2008

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The rice BAC-DNA was used as probes and fluorescence in situ hybridization (FISH) was applied to the interphase and metaphase mitotic chromosomes of maize. To optimize the BAC-FISH technique, we respectively assayed the effect of several factors, including maize or rice genomic C o t DNA used as blocking reagent of DNA, washing temperatures and FAD concentration in the washing buffer and in the hybrid solution. The results show that C o t DNA of maize genome blocked the repetitive sequence of the rice BAC-DNA when the C o t value was below 50. Meanwhile, it was necessary to adjust the C o t value according to the different probes and their ratios. Decreasing the concentration of FAD in the hybridization mixtures, adjusting the washing rate after hybridization, and most especially, blocking the ricespecific repetitive sequences of BAC-DNA could improve the positive signals of BAC-FISH.

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The physical Location of Genes cdc2 and prh1 in Maize (Zea Mays L.)

Cited by 17 publications

References 52 publications

A study of crop-to-crop gene flow using farm scale sites of fodder maize (Zea mays L.) in the UK

A study of crop-to-crop gene flow using farm scale sites of fodder maize (Zea mays L.) in the UK

Comparative analysis of genomes in Oryza sativa, O. officinalis and O. meyeriana with C 0 t-1 DNA and genomic DNA of cultivated rice

Technological exploration of BAC-FISH on mitotic chromosomes of maize

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