The physical basis of fabrication of amyloid-based hydrogels by lysozyme

Kumari, Anumita; Ahmad, Basir

doi:10.1039/c9ra07179b

Cited by 13 publications

(9 citation statements)

References 47 publications

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“…The amount of sulfhydryl groups carried by BSA is low, so it is difficult to form hydrogel network through sulfhydryl cross‐linking. Therefore, TCEP, a reducing agent with excellent reduction efficiency and good biocompatibility, was selected to reduce the disulfide bond on BSA [29] . In this paper, TCEP was used to open the original disulfide bond of BSA to produce more sulfhydryl groups, so that new disulfide bonds could be formed among multiple BSA, thus forming the first network through cross‐linking [29,30] .…”

Section: Resultsmentioning

confidence: 99%

“…In this paper, TCEP was used to open the original disulfide bond of BSA to produce more sulfhydryl groups, so that new disulfide bonds could be formed among multiple BSA, thus forming the first network through cross-linking. [29,30] By adding PVA as the second network, the two are cross-linked to form BP double network hydrogel, which makes up for the deficiencies of insufficient mechanical strength, limited hydrophilicity and insufficient elasticity of the single PVA hydrogel network. On the basis of BP hydrogel preparation, BPE double network hydrogel was prepared by adding different concentrations of EPL (Figure 1).…”

Section: Preparation Of Bp Hydrogel and Bpe Hydrogelmentioning

confidence: 99%

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Bovine Serum Albumin/Polyvinyl Alcohol Double‐Network Hydrogel Containing ϵ‐Polylysine for Antibacterial Performance

Wang,

Shi,

Zhang

et al. 2024

ChemistrySelect

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With the discovery and abuse of antibiotics, bacterial resistance has become a key problem in the field of antibacterial materials. Therefore, antibacterial materials that can effectively inhibit bacterial infection are considered to be important biological materials. In this study, a double‐network hydrogel system was designed using bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as the main body. The first network was formed by reducing the disulfide bond on BSA to sulfhydryl group by tris (2‐carboxyethyl) phosphine (TCEP). The second network of PVA was used as the hydrogel to prepare a double‐network hydrogel with good mechanical strength. The introduction of ϵ‐polylysine gives the hydrogel antibacterial activity, which enables it to destroy the cell structure and kill bacteria through electrostatic interaction, and can effectively solve the problem of drug resistance in the field of antibacterial medicine.

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Bp Hydrogel and Bpe Hydrogelmentioning

confidence: 99%

Bovine Serum Albumin/Polyvinyl Alcohol Double‐Network Hydrogel Containing ϵ‐Polylysine for Antibacterial Performance

Wang,

Shi,

Zhang

et al. 2024

ChemistrySelect

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“…The result was a lysozyme hydrogel optically transparent at lower molar ratios of TCEP/lysozyme converted into turbid gels. The turbidity of the gels decreases with the increase in the concentrations of TCEP [ 138 ]. Heat at 60 °C with TCEP addition leads to significant perturbation in the structure of the lysozyme folded state due to reduction of all four disulphide bonds present in the lysozyme structure [ 139 ].…”

Section: Protein Based Metamaterialsmentioning

confidence: 99%

“… Schematic diagram of protein based metamaterials. ( a ) Protein films of silk fibroin nanopatterned 2D lattices that exhibit different colors as a function of varying lattice spacing [ 98 ], ( b ) Protein affinity interactions direct the self-assembly of metamolecules integrated by NPs tightly packed around a single dielectric core [ 115 ], ( c ) Protein amyloid fibrils induction by chemical and thermal denaturation, site directed adsorption and subsequent reduction of precursor salt inducing the growth of NP´s nanoparticles aligned regularly into amyloids fibrils [ 132 ], ( d ) Formation of protein amyloid based hydrogel by reduction, concentration variation and thermal denaturation process [ 138 ], ( e ) Coated Protein based self-assembly of metal/metal plasmonic core/satellite nanoclusters [ 143 ]. …”

Section: Protein Based Metamaterialsmentioning

confidence: 99%

“…Egg proteins have been applied in many fields due to their foaming, coagulative, emulsifying, antimicrobial and structural intrinsic properties [ 147 ]. In the area of optical metamaterials, lysozyme has been used in hydrogel fabrication with transparent or cloudy optical appearance depending on lysozyme concentration [ 138 ]. Conalbumin has been applied for the construction of nanoclusters with different optical/plasmonic properties by changing particle size, composition, or assemblies ( Figure 3 e) [ 143 ].…”

Section: Protein Based Metamaterialsmentioning

confidence: 99%

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Biomolecule-Based Optical Metamaterials: Design and Applications

et al. 2022

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Metamaterials are broadly defined as artificial, electromagnetically homogeneous structures that exhibit unusual physical properties that are not present in nature. They possess extraordinary capabilities to bend electromagnetic waves. Their size, shape and composition can be engineered to modify their characteristics, such as iridescence, color shift, absorbance at different wavelengths, etc., and harness them as biosensors. Metamaterial construction from biological sources such as carbohydrates, proteins and nucleic acids represents a low-cost alternative, rendering high quantities and yields. In addition, the malleability of these biomaterials makes it possible to fabricate an endless number of structured materials such as composited nanoparticles, biofilms, nanofibers, quantum dots, and many others, with very specific, invaluable and tremendously useful optical characteristics. The intrinsic characteristics observed in biomaterials make them suitable for biomedical applications. This review addresses the optical characteristics of metamaterials obtained from the major macromolecules found in nature: carbohydrates, proteins and DNA, highlighting their biosensor field use, and pointing out their physical properties and production paths.

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Functional Amyloids: The Biomaterials of Tomorrow?

Peña‐Díaz,

Olsen,

Wang

et al. 2024

Advanced Materials

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Functional amyloid (FAs), particularly the bacterial proteins CsgA and FapC, have many useful properties as biomaterials: high stability, efficient, and controllable formation of a single type of amyloid, easy availability as extracellular material in bacterial biofilm and flexible engineering to introduce new properties. CsgA in particular has already demonstrated its worth in hydrogels for stable gastrointestinal colonization and regenerative tissue engineering, cell‐specific drug release, water‐purification filters, and different biosensors. It also holds promise as catalytic amyloid; existing weak and unspecific activity can undoubtedly be improved by targeted engineering and benefit from the repetitive display of active sites on a surface. Unfortunately, FapC remains largely unexplored and no application is described so far. Since FapC shares many common features with CsgA, this opens the window to its development as a functional scaffold. The multiple imperfect repeats in CsgA and FapC form a platform to introduce novel properties, e.g., in connecting linkers of variable lengths. While exploitation of this potential is still at an early stage, particularly for FapC, a thorough understanding of their molecular properties will pave the way for multifunctional fibrils which can contribute toward solving many different societal challenges, ranging from CO2 fixation to hydrolysis of plastic nanoparticles.

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The physical basis of fabrication of amyloid-based hydrogels by lysozyme

Abstract: Schematic of heating- and cooling-induced transitions between HEWL states, and the subsequent formation of the hydrogel.

Cited by 13 publications

References 47 publications

Bovine Serum Albumin/Polyvinyl Alcohol Double‐Network Hydrogel Containing ϵ‐Polylysine for Antibacterial Performance

Bovine Serum Albumin/Polyvinyl Alcohol Double‐Network Hydrogel Containing ϵ‐Polylysine for Antibacterial Performance

Biomolecule-Based Optical Metamaterials: Design and Applications

Functional Amyloids: The Biomaterials of Tomorrow?

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