The phylogeny of the genus Nannochloropsis (Monodopsidaceae, Eustigmatophyceae), with descriptions of N. australis sp. nov. and Microchloropsis gen. nov.

Fawley, Marvin W.; Jameson, Ian; Fawley, Karen P.

doi:10.2216/15-60.1

Cited by 76 publications

(61 citation statements)

References 35 publications

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“…Previously collected genomic DNA samples from other Eustigmatophyceae strains were also used to generate new rbcL sequences to complete a data set for that locus similar to the taxon set used in Fawley et al (2014). Conditions for polymerase chain reaction amplification were as described in for the nuclear 18S rDNA region and Fawley et al (2015) or Prior et al (2009) for the plastid rbcL gene. DNA sequencing was performed as described in Fawley et al (2015), with sequencing done by Sequetech (Mountain View, California, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Conditions for polymerase chain reaction amplification were as described in for the nuclear 18S rDNA region and Fawley et al (2015) or Prior et al (2009) for the plastid rbcL gene. DNA sequencing was performed as described in Fawley et al (2015), with sequencing done by Sequetech (Mountain View, California, USA). Sequence reads were joined using the Staden Package 2.0.0b8.…”

Section: Methodsmentioning

confidence: 99%

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Rediscovery of Tetraëdriella subglobosa Pascher, a member of the Eustigmatophyceae

Fawley¹,

Fawley²

2017

Fottea

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An algal strain from an acidic pool near the shore of Lake Mácha, Czech Republic, was identified as Tetraëdriella subglobosa Pascher by light microscopy. Phylogenetic analysis of nuclear 18S rDNA and plastid rbcL sequence data indicated that this alga is a member of the Eustigmatophyceae, rather than the Xanthophyceae as presently classified. This is the first report of T. subglobosa since Pascher's description of the species in 1930.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Rediscovery of Tetraëdriella subglobosa Pascher, a member of the Eustigmatophyceae

Fawley¹,

Fawley²

2017

Fottea

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“…Hence, it is presently unclear whether the acquisition of the operon preceded the radiation of the whole Eustigmatales group or whether it occurred within a particular Eustigmatales lineage. Importantly, the absence of the ebo operon from all sequenced plastid genomes of the genera Nannochloropsis and Microchloropsis , together constituting a sister group to the Monodopsis lineage [7], implies a secondary loss of the operon from that group (figure 1). …”

Section: Resultsmentioning

confidence: 99%

The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite

et al. 2016

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Acquisition of genes by plastid genomes (plastomes) via horizontal gene transfer (HGT) seems to be a rare phenomenon. Here, we report an interesting case of HGT revealed by sequencing the plastomes of the eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria sp. CAUP Q 202. These plastomes proved to harbour a unique cluster of six genes, most probably acquired from a bacterium of the phylum Bacteroidetes, with homologues in various bacteria, typically organized in a conserved uncharacterized putative operon. Sequence analyses of the six proteins encoded by the operon yielded the following annotation for them: (i) a novel family without discernible homologues; (ii) a new family within the superfamily of metallo-dependent hydrolases; (iii) a novel subgroup of the UbiA superfamily of prenyl transferases; (iv) a new clade within the sugar phosphate cyclase superfamily; (v) a new family within the xylose isomerase-like superfamily; and (vi) a hydrolase for a phosphate moiety-containing substrate. We suggest that the operon encodes enzymes of a pathway synthesizing an isoprenoid–cyclitol-derived compound, possibly an antimicrobial or other protective substance. To the best of our knowledge, this is the first report of an expansion of the metabolic capacity of a plastid mediated by HGT into the plastid genome.

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“…Microchloropsis gaditana CCMP526, previously known as Nannochloropsis gaditana CCMP526, is one of the six algal species from the genus Nannochloropsis (Fawley et al, 2015). It is a marine microalga known for high oil accumulating capability and especially its eicosapentaenoic acid (EPA) content.…”

Section: Introductionmentioning

confidence: 99%

A Data-Independent-Acquisition-based proteomic approach towards understanding the acclimation strategy ofMicrochloropsis gaditanaCCMP526 in hypersaline conditions

Karthikaichamy

Beardall

Coppel

et al. 2020

Preprint

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Salinity is one of the significant factors that affect growth and cellular metabolism, including photosynthesis and lipid accumulation, in microalgae and higher plants. Microchloropsis gaditana CCMP526 can acclimatize to different salinity levels by accumulating compatible solutes, carbohydrates, and lipids as an energy storage molecule. We used proteomics to understand the molecular basis for acclimation of M. gaditana to increased salinity levels (55 and 100 PSU (Practical Salinity Unit). Correspondence analysis (CA) was used for the identification of salinity-responsive proteins (SRPs). The highest number of altered proteins was observed in 100 PSU. Gene Ontology (GO) enrichment analysis revealed a separate path of acclimation for cells exposed to 55 and 100 PSU. Osmolyte and lipid biosynthesis was up-regulated in high saline conditions. However, concomitantly lipid oxidation pathways were also up-regulated at high saline conditions, providing acetyl-CoA for energy metabolism through the TCA cycle. Carbon fixation and photosynthesis were tightly regulated, while chlorophyll biosynthesis was affected under high salinity conditions. Importantly, temporal proteome analysis of salinity-challenged M. gaditana revealed vital salinity-responsive proteins which could be used for strain engineering for improved salinity resistance.

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The phylogeny of the genus Nannochloropsis (Monodopsidaceae, Eustigmatophyceae), with descriptions of N. australis sp. nov. and Microchloropsis gen. nov.

Cited by 76 publications

References 35 publications

Rediscovery of Tetraëdriella subglobosa Pascher, a member of the Eustigmatophyceae

Rediscovery of Tetraëdriella subglobosa Pascher, a member of the Eustigmatophyceae

The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite

A Data-Independent-Acquisition-based proteomic approach towards understanding the acclimation strategy ofMicrochloropsis gaditanaCCMP526 in hypersaline conditions

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