The phthalocyanines applications in photodynamic therapy investigated by time‐resolved and steady‐state photothermal methods

Fra̧ckowiak, D.; Wiktorowicz, Krzysztof; Planner, A.; Waszkowiak, Aneta; Ion, Rodica‐Mariana

doi:10.1155/s1110662x02000090

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…A similar absorption was observed in the incubation solvent (Me 2 SO) (not shown). This is a typical absorption pattern of Pcs making them good candidates for PDT (Fr¹ckowiak et al, 2001b;2001d). The main fluorescence emission maxima, at 337 nm excitation, were located in the 650-800 nm region (Fig.…”

Section: Resultsmentioning

confidence: 74%

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Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.

Waszkowiak

Fra̧ckowiak²,

Wiktorowicz³

et al. 2002

Acta Biochim Pol

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Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalocyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.

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Section: Resultsmentioning

confidence: 74%

“…In our previous investigations on PDT sensitizers (Fr¹ckowiak et al, 1996;Ion et al, 1998;Fr¹ckowiak et al, 2001a;2001b;2001d) we have used several spectroscopic methods. The investigated Pcs exhibited various, but always rather high, efficiency of triplet state generation.…”

mentioning

confidence: 99%

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.

Waszkowiak

Fra̧ckowiak²,

Wiktorowicz³

et al. 2002

Acta Biochim Pol

Self Cite

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“…Photodynamic therapy (PDT) is a promising therapeutic technique for the treatment of cancerous or other unwanted tissues based on the combined use of light irradiation and photosensitizer (PS) [1][2][3][4][5][6][7][8][9]. After photoactivation of PS, the interaction between the excited triplet PS and ground-state triplet oxygen ( 3 O 2 ) generates singlet oxygen ( 1 O 2 ), which is the main cytotoxic species that can lead to photodynamic damage of tumor cells and tissues [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorinfin Human Breast Cancer Cells: A Diffusion Kinetics Study

Chen

Zhang

et al. 2012

International Journal of Photoenergy

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The kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorinf(CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubation time, which was followed by its binding to cell organelles. Subcellular distribution study revealed that CPD4 was primarily localized on the mitochondria and membranes, supporting that the mode of transmembrane transport was diffusion. A kinetics model describing CPD4 passing through the plasma membrane of MCF-7 cells was proposed based on Fick's first law of diffusion. The kinetics of cellular uptake of CPD4 was studied by three-dimensional LSCM. By fitting the experimental data using the above model, important cellular uptake and distribution parameters were obtained, which are of clinical significance in photodynamic therapy.

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Spectral and Aggregation Properties of Phenylthio-Substituted Al-Phthalocyanine Molecules in Nanoporous Silicate Matrices

et al. 2022

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The phthalocyanines applications in photodynamic therapy investigated by time‐resolved and steady‐state photothermal methods

Cited by 8 publications

References 5 publications

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.

Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorinfin Human Breast Cancer Cells: A Diffusion Kinetics Study

Spectral and Aggregation Properties of Phenylthio-Substituted Al-Phthalocyanine Molecules in Nanoporous Silicate Matrices

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